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Dicarbocyanine Lab Report

Satisfactory Essays

The spectrometer was calibrated using a “blank” cuvette of methanol and then the absorbance and the wavelength for each dye was measured. Each dye was diluted tenfold several times with methanol until the max wavelength peak had an absorbance less than 1. All three dyes needed to be diluted 3 times before they were at the correct concentration to measure the maximum wavelength. As each dye was diluted the colors of the dyes became more faded and also a lighter shade, for example 1-1’-diethyl-2-2’-cyanine iodide, which was red initially became a shade of pink. The initial colors observed for pinacyanol chloride was blue and 1-1’-diethyl-2-2’-dicarbocyanine iodide was green. Graphs of the absorbance versus wavelength (nm) for all three dyes

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