Difference Between Behavior And Histology Of Pbs Or Lps Treated Mice
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4.1 Review of results
Contrary to what we expected, there was no significant difference between behaviour and histology of PBS or LPS treated mice. Some results, however, such as the number of microglial clusters, may benefit from an increase in the n number, since the lack of significance was caused, partially, due to high variability inside the groups. We are currently evaluating the immunohistochemistry of 4 mice, although the initial idea of a profound effect of intranasal LPS over behaviour and histology seem improbable. This can have several factors which are going to be explored individually.
4.2 Post-infection Time Window
Intranasal infection with laboratory isolate of Chlamydia pneumonia was shown to induce immune reaction in the olfactory bulb and brain at 1 month post-infection (pi), which gradually declined, but was still detectable, at 3 and 4 months (Little et al., 2014). On our experiment, tissue collection was done roughly 2 weeks after the LPS injections, therefore, there may have not been enough time for development of immune response, such as microgliosis. Nevertheless, in vitro studies, which exposed neuronal cells to Borrelia burgdorferi bacteria, as well as LPS, detected increased β-amyloid and hyperphosphorylated tau as early as 2 weeks after exposure (Miklossy et al., 2006). In contrast, the in vivo study with Chlamydia pneumonia failed to find tau pathology and suggested that long term exposure to pathogen (>1 year) may yield better