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Diffusion of Water Across Plasma Membrane Of RBC'S

Satisfactory Essays
b.)Purpose- The purpose of this experiment is to understand diffusion osmosis. c.)Hypothesis- Slide #1 blood only is isotonic, Slide #2 Blood plus .9% of NaCl is Hypotonic, Slide 3# blood plus 10% of NaCl is Hypertonic and Slide #4 Sheeps blood and dH20 is Isotonic. II.Procedure- a.)Equipment Used-

What we need for this experiment
0.9% NaCl
Filter paper
4 clean slides
10% NaCl
Wooden applicator stick
Cover-slips
dH20
1 disposable mat
Microscope
Sheeps blood
Disposable gloves goggles b.)Collection of Data-
Step 1: Make sure you have all of your supplies in front of you. Have all four slides labeled one through four.
Step 2(PART 1 & PART 2): PART 1: Each student takes a slide. Each student also needs one cover-slip and filter paper (for excess liquid) PART 2: Read what you are to do before you do it.

Step 3: DO AS TOLD BELOW. DO IT QUICK AND DILIGENTLY. BLOOD WILL DRY OUT QUICKLY.

Student with slide #1: Use the stick and place a small drop onto each of the slides.
Student with slide #2: Place a drop of 0.9% Nacl on top of the sheeps blood. Cover it with the slip and Clean extra off with the filter paper. ( Not needed , if no spill)
Student 3# Place a drop of 10% NaCl solution on top of the blood. Cover it with the slip and clean the extra off with the Filter paper.
Student 4:Place a drop of dH20 on top of the blood. Cover it with the slip and clean the extra off with filter paper.
Step 4: Look at the slide under 40x , 100x , and high power 400x. Draw
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