Diffusion of Water Across Plasma Membrane Of RBC'S

Explain what happened to the blood cells at the various levels of concentration. Be sure to refer to the solutions as being hypotonic, hypertonic and isotonic.

2. Read the background information found under the “Question” area first, and then continue on to reading the “Procedure” information posted there as well.

Please note that in the presentation_content file, the person who has drafted the presentation has left you some instructions in red.

2 Make a multiple exposure test strip under image enlarger as usual, process as usual and determine best exposure time.

In order to conduct this experiment you will need to know all the major steps. First, you will grab the depression slide and use your eyedropper to collect a single Daphnia and place it in the indentation of the depression slide. Make sure to remove excess water with the corner of your small paper towel. No coverslip will be necessary.

Seal your bag and mix everything carefully. You now have a bag of blood you can take home!

XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.

During this lab, we investigated the relationship between the surface and the appearance of the blood drop. The thinner the material of the surface, the more the blood drop absorbed into the paper. The height of the drop and the angle of impact were also contributing factors. The diameter of a drop increased with the height of the drop. In addition, we found that when a blood drop hits the surface at a 90 degree angle, a circular stain is produced. As the degree of impact decrease, the blood drops begin to trail towards the bottom of the

Load the remaining protein mix into the column, and this will begin to bind within the column. Once the solution has settled, fill the column with NaCl a total of four times; as the wash is flowing through, collect and label the protein solution in fractions, and save for the

First get your 4 pictures. We found these at www.exploratorium.edu. Then you will need a pink and green see through clipboard that is 8.5 by 11 inches. Before you start the experiment you need to have a clean room. Next find four twelve year olds, two boys, two girls. Then you need to collect the rest of the materials.

Submerge the graduated cylinder in the plastic tub so that it is completely filled with water. Hold the open end of the graduated cylinder and move it vertically upside-down where the open end of the graduated cylinder is still submerged in the plastic tub. Clamp the graduated cylinder the ring stand of the lab table to keep it in place. perforate a hole in the top of the rubber cork for the solution container. Cut a straw the length of about four inches. place the straw inside of the rubber cork hole. Set up your timer for two minutes.

Place the other glass slide on top of the first, and gently pull the slides apart − dispensing the material on the slides evenly.

The purpose of this experiment was to determine the effects of tonicity on a cell membrane using red blood cells, potato strips and three unknown solutions (A, B, C). First three slides were prepared containing RBC’s and unknown solutions A, B and C. A control slide was prepared only using RBC’s. After observing each slide under the microscope it was determined that unknown solution A was hypertonic because the RBC appeared to have shrunk. The RBC in unknown solution B appeared to be swollen, therefor, the tonicity of unknown solution B was hypotonic. Unknown solution C showed no change to the RBC shape, it was suggested that unknown solution C was isotonic. To confirm the tonicity

3. Touch and hold the edge of the test strip to the drop of blood, and wait for the result.

Finally, in an isotonic solution there will not be any change in the shape of the red blood cells as there is no net movement of water in or out of the red blood cells.