Does Caffeine Affect Heart Rate?

This experiment is to see The Affect of Caffeine on Daphnia. Daphnia is also known as a water flea. It is a distant relative of crabs and shrimps. (Marshall Cavendish, 2002) Daphnia have a hard external skeleton with jointed appendages and limbs. The head contains a large central eye which is made of two eyes joined together. Daphnia has two pairs of antennae which are used for swimming. Each daphnia has five pairs of limbs. These limbs lie in a certain space under the body bounded by the carapace. (Marshall Cavendish, 2002) A carapace is a hard upper shell or chitinous outer covering on the back of some animals. The daphnia is well preserved because of its shells which are composed of chitin. The usual size of this microorganism is about 0.2-0.3 mm; they look like flat disks. Their bodies are divided into three parts – head, thorax, and abdomen. The head is typically dome-shaped with five pairs of appendages. Among these five are two pairs of antennae; there is a small pair and a larger pair. The smaller pair of antennae serves as a sensory function and the larger one is used for swimming. The other three appendages’ purpose is to secure food they intake. (Michael Hutchins, 2003) The thorax holds four to six pairs of legs that are used for gathering food, filtering water, or grasping mates. There are over 400 species of daphnia and they are distributed worldwide. Daphnia uses their thoracic legs to produce a constant current of water; this allows them to filter food

​The data shows that when the temperature of the water surrounding the deep well slide containing the Daphnia magna increased, the heart rate of the Daphnia magna increased, and when the temperature of the water decreased, the heat rate of the Daphnia magna decreased as well. For example, when the water was at room temperature, 21° C, the average heart rate was 393.33 BPM. When the water temperature was increased to 30°C, the average heart rate increased to 510.66 BPM. When the water temperature was decreased to 10° C, the average heart rate in beats per minute decreased to 332 BPM, less than both the 21° C and 30° C results. The warmer the water temperature was, the higher the heart rate of the Daphnia magna.

Preparing specimen for electron microscope hard, light microscope still very useful as a window on living cells.

This lab covered the effects of caffeine, nicotine, caffeine extract and nicotine extract on the pulsation rates of Lumbriculus variegatus, commonly known as blackworms. The circulatory system consists of the heart and the blood vessels that circulate blood throughout the organism’s body. Blackworms do not possess a respiratory system or a heart, thus they circulate their blood through contractions of the blood vessels. The pulsation rate was observed along the dorsal blood vessel near the posterior end of the worm to give better readings. The various drugs were exposed to the black worm and the effects it had on the pulsation rate were observed. The results found in this experiment could also relate to the effects of these

To perform this test, a small drop of water is placed on a clean microscope slide. A metal loop that has been properly sterilized in the blue flame and allowed time to cool is used to

1) Apply the stain to your first unknown slide and examine it under the microscope.

4. Identify the endocrine organ from which a slide is prepared when viewing the slide with a microscope.

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

To begin the experiment, we took a single Daphnia magna from the unused tank to run control tests with distilled water on the untampered heart rate of the species. The species was placed on a concave slide with a new drop of water on it. It was given 2 minutes of recovery time to adjust to its new environment; then, the heart rate was studied by intervals of 15 seconds to find beats per minute by multiplying the number of beats in 15 seconds by 4. The Daphnia magna was studied under a light microscope with 40x total magnification. After each round of data collection, the water was absorbed with a Kimwipe so a new water drop could be placed on the species. The same Daphnia magna was used for six rounds of control experiments. After

We could see that is was transparent and observed movement inside the specimen. We could see a green circle inside the specimen. Specimen #2

The investigator will examine duct tape piece “A” against duct tape pieces “B”, “C”, and “D” to determine similarities and differences between them utilizing increasingly strong microscopy methods—beginning with the unaided eye, then utilizing a magnifying glass, and ultimately a stereomicroscope. While using each of the above methods, the investigator will carefully note observations made with each in his or her lab notebook. These observations will come in the

The eggs were each individually placed in one of the beakers and were taken out and weighed every 15 minutes for 60 minutes. Weighing the eggs helped to test the process of osmosis by finding out the mass before and after soaking in water, this will dictate whether water has moved in or out of the egg.

The materials used for the first part of the experiment comprised of the following: a microscope, 4 slides, 4 slide covers, blood samples, lancet, a sheet of paper towel, 3 test tube droppers, Solutions A, Solutions B, and Solution C.

They made use of a unique visualisation technique in order to see the neuromast cupulae (a jelly-like layer on the face area which aids in the processing of movement detection) of the two groups of adult cavefish. The fish were anesthetised and then this delicate layer was gently removed and glued to a clean glass slide, which enabled viewing under a specific kind of microscope. Pictures were taken using a special camera at various magnifications. These highly magnified images were then used to record features of mechanical importance.

NOTE: Answer Question A only if you used a compound light microscope for this experiment.