Dyes And Crimes Lab Report

After the serial dilutions of the red and blue dyes were taken, the molarity and absorbance for both dyes were calculated. Using the MiVi = MfVf equation, the concentrations for each value of the red and blue dye were separately calculated. Calculating absorbances calls for setting the correct wavelengths of light for each dye. In this case, the 470 nm wavelength for red dye and the 635 nm wavelength for blue dye was needed to find the maximum absorbances. The absorbance was found by blanking the colorimeter and entering the concentrations. After both values of the absorbances and concentrations were found, the values were then graphed in order to obtain the equation of the relationship between absorbance and concentration.

The Uptake of Neutral red dye by saccharomyces cerevisiae in the presence of a metabolic inhibitor

Determining the concentration of an unknown dye and preparing an absorption spectrum for a known substance.

In this third experiment, I used different colors of filters within the laboratory and see how the wavelengths of light

In this lab a cotton swab was fluffed up by scraping it against the serrated edge of a tape dispenser and then dipped into a liquid chemical solution. Once dipped the cotton swab was placed horizontally above the fire making sure to only burn the cotton part so as to not accidently get a different flame because of the burning stick. The color of the flame is viewed through a spectroscope and then both that spectrum and the color to the naked eye are recorded on a data table. This step is repeated for the remaining four solutions and then the bunsen burner is turned off and the area is cleaned. The purpose of this lab was to observe the relationship between various elements and emission spectroscopy and identify an unknown substance. By burning

The development of Crime labs allowed law enforcement to investigate, arrest, and convict individuals who have broken the lab. By creating crime labs it allowed law enforcement agencies to learn from each other by documenting and researching similar crimes it allows them to follow similar techniques in order to catch the suspects.

Thin Layer Chromatograph works using the same principle as all chromatographic methods. In TLC the stationary phase used is a thin layer usually of silica gel or a sheet of material coated in alumina and the mobile phase is a set saturation of any suitable liquid containing the mixture we wish to separate. It is set up so that the stationary phase is upright and placed on a low level of the liquid mobile phase, marking where this liquid reaches with a pencil line, on that pencil line a spot of the mixture to be separated is placed. As the mobile phase is absorbed by the stationary phase, or the TLC plate, the mixture should travel up also separating into its components depending on their attraction to the TLC plate as it rises. It is possible in TLC to use pre coated thin layer sheets or to prepare the sheets

In the past decades lasers have become evidently important to society. Lasers are normally perceived as devices used by our hands or large boxes found in research laboratories. Another evolvement in science that has taken place is the use of fluorescent dyes. Fluorescent dyes have become a common use for research and diagnostics in order to identify specific cell and tissue types. The use of such a dye is to identify for example, various chemical substances such as proteins, tag cells and DNA and their concentration. The only problem with such dyes is only as few as 10 colours have the ability to be distinguished. These newly developed lasers formed by fat cells are a combination of solid styrene beads. The purpose for this evolution is to capture light that is taken from the cells which are views using a spectrometer and thus, analyze the spectrum. It serves a function of measuring refractive index change. Before this step forward the ability to tag cells stood at around a couple hundred cells. Fat cells already consist of lipid droplets and act as natural lasers as it is. They need nothing more than to be supplied with nontoxic fluorescent dye. This new laser technology can help us further understand the cellular process and further improve medical diagnosis and therapies. Considering the level of

There are four different kinds of chromatography, there is gas, paper, liquid, and thin-layer. Gas chromatography is mostly used to determine the chemical composition of the unknown substances. Thin-layer is used on thin plastic or glass trays to identify the composition of pigments and chemicals. Liquid is used to identify an unknown plant pigments and other compounds. Lastly, paper can be used to separate the components of inks and dyes. So in this essay i will talk about two real life cases that involve Gas and paper chromatography and

The objective of this experiment was to investigate the concept of paper chromatography and attempt to achieve a clear separation of food dyes found in M&M’s and to compare and evaluate the dyes found. Chromatography is derived from the greek words “chroma” meaning color and “graphein” meaning to write.

Colors affect the intensity of sunlight. The purpose of this lab was to see how different colors affect the intensity of the light in a given solution. During the lab, a calorimeter probe was used to study the relationship between concentration and light transmittance through solution samples and how light transmittance varies with the wavelength of light. In a calorimeter, specific light of a substance and intensity is passed through a solution. A detector then measures the amount of light that comes out of the other side. The light that is not transmitted is absorbed by the sample. If the concentration of the sample is greater then the intensity of light that passes through the sample is lower. The ratio of the intensity of light leaving

In this experiment, the concepts of serial dilutions and the absorbency of light were explored and analyzed. Calibration curves were also used that consisted of the λmax and absorbances of standards. In addition, spectrophotometry was used to determine the concentrations of

Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distanced when eluted with the proper solvent. According to NCBI, this is because the more polar compound has a stronger interaction with the silica gel due to their polar properties, making it easier to drive the eluent away from its binding area. On the other hand, nonpolar compounds generally consist of van der Waals dipole interactions. Therefore, they don’t have as much resistance going down the silica gel and will travel further. The compounds can also be determined by comparing the substances retention factor 〖(R〗_f) with a known compounds R_f value. The R_f value is calculated by the following equation,

Paper chromatography is a method used to separate colour soluble substances ink. There are two phases: mobile phase and stationary phase. The stationary phase is chromatography paper. The mobile phase is the solvent that travels up the stationary phase with the samples. In the process, a dot of sample ink is placed on the line of origin (Wikipedia, 2011). After the chromatography paper touch the solvent, the solvent will travel up slowly and separate the component. In paper chromatography, the attraction of soluble substances between the solvent and paper varies with more strongly attracted substances moving more slowly up the paper than those less strongly attracted to the paper (Refer to Figure 1).

Recently, Natural organic dyes like indigo and indigo carmine are widely imported extensively in many industrial issues like textiles, printing, dying, and food [1]. Indigos Family are characterized by their high stability that arises from inter/intramolecular hydrogen bonding. The electronic and vibronic spectra of indigos are strongly influenced by π-domains intermo-lecular interactions [2]. Also, Indigos are well known of their high photochromicty which enrich their potential applications in photonic, storage, and spintronic devices [3]. Indigo carmine (IC), or 5,5′-indigodisulfonic acid sodium salt, is an organic salt derived from indigo by sulfonation, which renders the compound soluble in water. It is approved for use as a food colorant in the U.S and E.U. It has the E number E132. Indigo carmine is primarily employed as a pH indicator. It is blue at pH 11.4 and yellow at 13.0. Also, it is a redox indicator [4-6]. Indigo Carmine (IC) has a chemical formula of C16H8N2Na2O8S2, Molar mass (466.36 g/mol), and melting point (>300 °C). Moreover, Its other uses include indicating dissolved ozone through the conversion to isatin-5-sulfonic acid [7] and detecting superoxide, an important distinction in cell physiology [8] and being used as a dye in the manufacturing of capsules, and in obstetrics. Besides, the indigo carmine-based dye is used to detect