Effect Of A Substance On Daphnia

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.

This laboratory exercise was performed to visualize the effects of various drugs on the heart rate of Daphnia magna. The four drugs tested consisted of: Lidocaine, Acetylcholine, Caffeine, and Nicotine. These drugs were designed to have an apparent effect on the average heart rate of the Daphnia. The laboratory exercise was divided into two parts and procedures: measuring the basal heart rate of Daphnia, and measuring the drug induced heart rate of Daphnia. In order to measure the basal heart rate, various Daphnia were obtained and observed under a microscope at 40x magnification for two separate trials. Using the same technique, the drug induced heart rate with each drug (Lidocaine, Acetylcholine, Caffeine and Nicotine) was measured and

The effects of caffeine and alcohol on daphnia are expressive of whether these substances are harmful or beneficial to the organism. By understanding the results of this experiment, it may also be understood how these substances effect humans. In this study, one daphnia was exposed to increasing levels of alcohol, while the other was exposed to increasing levels of caffeine, each in order to test the hypothesis that when given amounts of caffeine and alcohol, the daphnia will be affected the same way a human would. The effect of each substance was measured by the daphnia’s heart rate one minute after the substance was added. Results reveal that alcohol slows the heart rate, while caffeine increases heart rate. Furthermore, caffeine shows a

Individual Daphnia magna are transferred to a microscopic slide using a pipette. The Daphnia was moving freely in a drop of H2O on the slide. For the control, the heart rate of D. magna is observed for 15 seconds

In this lab I did an experiment to see the effect of Ethanol on Daphnia. The purpose of this lab was to see is there effect of Ethanol on Daphnia’s heart rate. I used Daphnia for this experiment because it a transparent crustaceans meaning we see its heart and other stuffs from outside. In the experiment first, I measured the number of heartbeats of Daphnia without any treatment for 10 seconds. I measured the heartbeats for three times for no treatment, the average heart rate was 22. Seconds I putted 15% of Ethanol on Daphnia and waited for 30 seconds then I measured the number of heartbeats of Daphnia for 10 seconds. I measured the heartbeats for three times and the average of heart rate was 15. At last I found out that Ethanol does affect

In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.

For each test solution and base HR, the daphnia was submerged for ten-minutes to allow the chemicals to take effect, for daphnia are ectotherms and their body adapts to the surrounding environment. The data in Table 1 and Graph 1 answer the question of whether a daphnia, when exposed two depressants, i.e. melatonin and ethanol, will result in an average heart rate (HR) lower in comparison to the two depressants being administered individually. Over the three trials conducted for each of the test solution for daphnia 1, it can be concluded that 10% ethanol administration resulted in almost 2.6 fold, that is approximately 60%, decrease in HR. Therefore, ethanol had a depressant effect on average BPM, similar to Corotto's findings. (2010) Also, since the first daphnia died after

To conduct the experiment on the effects of caffeine and ethanol on the species, Daphnia magna, a set of instructions was followed laid out by the Shippensburg University of Pennsylvania Biology Department in the Laboratory Manual (Castle and Paulson

Immersing Daphnia in the ethanol indicated an increase in the heart rate. This relates to the fact that alcohol can have a big impact in the live of humans. Since the cross sectional part of the heart relates to that of humans. The increase in the heart rate enabled me to understand more about the how the increase in the heart rate when ethanol (Alcohol) is present in the body it can affect one's blood pressure [1]. In contrast, high blood pressure is somewhat indicates how one’s heart is beating really fast. This means that at as the heart is pumping blood through the vein and arteries in the body. The force the heart is using to pump the blood through the artery walls can cause a tear or damage of the arteries which can lead to death. Furthermore,

This experiment is being performed to show the effect of pH levels on daphnia by changing pH levels and measuring the heart rate.

The purpose of this paper is to compare and contrast how a toxin will affect the systems in the

In this experiment, the purpose was to identify the effect of sedatives and stimulants on the circulatory system on the ghost shrimp and the black worm. We used caffeine as our stimulant and ethanol as our sedative. The shrimp was placed on a wet petri dish, where it was covered. After letting the shrimp rest for a few minutes, we placed it under the lowest light of a dissecting microscope. Then we located the heart, counted the number of beats in 10 seconds and multiplied it by 6 to acquire the beats-per-minute. In the next trial we dropped 0.5 mL of .0005 M caffeinated solution on the shrimp and waited 2 minutes before we measured the bpm. After the bpm was recorded, the shrimp was washed with spring water. The same procedure was performed

Purpose of the experiment: To identify the whether or not caffeine increases or decreases the reaction time of the nervous system.

The snails were then placed in Petri dishes to be examined under a microscope to determine their heart rates. After 24 hours, the snails treated with caffeine concentration greater than 0.1% had weaker and irregular heart rates. All the snails that were treated with caffeine solutions 0.5% or 3% were dead after 96 hours.