Lab 4: Enzymes

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INSTRUCTIONS: • On your own and without assistance, complete this Lab 4 Answer Sheet electronically and submit it via the Assignments Folder by the date listed in the Course Schedule (under Syllabus). • To conduct your laboratory exercises, use the Laboratory Manual located under Course Content. Read the introduction and the directions for each exercise/experiment carefully before completing the exercises/experiments and answering the questions. • Save your Lab 4 Answer Sheet in the following format: LastName_Lab4 (e.g., Smith_Lab4). • You should submit your document as a Word (.doc or .docx) or Rich Text Format (.rtf) file for best compatibility. Pre-Lab Questions 1. How could you test to see if an enzyme was completely…show more content…
11. Wait five minutes. 12. Hold the IKI spray bottle 25 - 30 cm away from the paper towel, and mist with the IKI solution. 13. The reaction will be complete after approximately 60 seconds. Observe where color develops, and consider what these results indicate. Record your results in Table 1. Table 1: Substance vs. Starch Presence Substance Resulting Color Presence of Starch? Positive Control: Starch Dark Purple Yes Negative Control : Cellulose Brownish red color No Food Product: Apple Dark Purple yes Food Product: Potato Dark Purple yes Saliva: Amylase Brownish red color No Post Negative Control -Lab Questions 1. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment? 2. What is the function of amylase? What does amylase do to starch? 3. Which of the foods that you tested contained amylase? Which did not? What experimental evidence supports your claim? 4. Saliva does not contain amylase until babies are two months old. How could this affect an infant’s digestive requirements? 5. There is another digestive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to determine what this enzyme is called. What substrate does it act on? Where in the body does it become activated, and why? 6. Digestive enzymes in the gut include proteases, which digest proteins. Why don’t these enzymes digest the stomach and small intestine,
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