Enzymes Lab Report

2. We measured 1 mL of turnip peroxidase (the enzyme) and 3 mL of neutral buffer (pH corresponding to the test tube number i.e. pH 5 in test tube 5) with a syringe and disposed it into tubes 3, 5, 6, 7, 8, and 10

The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.

As mentioned in question two, pH is another important factor regarding how active an enzyme could be. Although not all enzymes have the same optical pH levels, a majority of enzymes work the most effectively when the pH of their environment is between six and eight. If most enzymes are put in more acidic or alkaline environment, the enzymes would no longer function and would be irreversibly damaged. For example, the enzymes would not form the proper shape to bond with the substrates or they would not be able to bond with the substrates. As a result, the imbalance of the pH level would inhibit the rate of reaction since reactions would no longer be able

Introduction Enzymes serve important roles in the biological processes that are undertaken in human bodies. Enzymes are most important as their role as proteins which speed up chemical reactions in the body, which make them catalytic proteins (Agarwal, 2006). The unique structure of enzymes allows them to fit into a specific type of chemical, called a substrate (Robinson, 2006). The functionality of an enzyme is determined by its active site. The active site of an enzyme is the location on the enzyme where the substrate binds to produce a substance (Robinson, 2006).

Abstract Enzymes are organic catalysts that can help speed up chemical reactions (enzymes function p57). There are very few exceptions, however all enzymes are proteins. Every enzyme is specific to a certain chemical reaction depending on its substrate as well as amount (enzyme function p57). Enzymes must maintain a specific structure so that they can work properly. If an enzyme's structure is changed by chemicals or heat it may not be able to function at all.

“Enzymes are proteins that have catalytic functions” [1], “that speed up or slow down reactions”[2], “indispensable to maintenance and activity of life”[1]. They are each very specific, and will only work when a particular substrate fits in their active site. An active site is “a region on the surface of an enzyme where the substrate binds, and where the reaction occurs”[2].

An enzyme works by binding

Each enzyme is selectively specific for the substance in which it causes a reaction and is most effective at a temperature particular to it. Although an increase in temperature may accelerate a reaction, enzymes are unstable when heated. ‘Many enzymes require the presence of another ion or a molecule called a cofactor’ (Mosbach, p.192), in order to function. As a rule, enzymes do not attack living cells. As soon as a cell dies, however, enzymes that break down protein rapidly digest it. The resistance of the living cell is due to the

The objective for performing this set of experiments is to get a better understanding of how enzymes work. By experimentally altering the conditions of the enzyme we are able to identify how it functions best. This information can be applied to the cellulosic ethanol process, resulting in new and improved methods for the most efficient ways to create biofuels. The use of public transportation in America has grown tremendously over the years.

Enzymes are catalytic proteins that accelerate the rate of biological reactions while experiencing no permanent chemical modification as a result of their participation in a reaction. In order to initiate a reaction from a reactant called a substrate to a product, a certain amount of energy, otherwise known as the activation energy, is required. An enzyme functions by lowering the required activation energy (which is usually provided by heat), thus, expediting the reaction. Many chemical reactions happen very slowly, without the help of enzymes some reactions could take up to 3 billion years to occur. Enzymes increase the rate of reactions by a factor up to 1017 times, allowing the chemical reactions that make life possible to take place at

The uniqueness is due to the shapes of the enzymes. Most enzymes are made of a protein and a non-protein, usually called a cofactor. The molecular bonds that hold the proteins in their secondary and tertiary structures are

Below is the graph that shows the mean volume of water displaced due to the oxygen produced when spinach catalase of pH levels 4, 6 and 8 reacted with hydrogen peroxide respectively. Analysis of Data: Graph 1 is a visual representation of the data collected. When pH buffer solution of 4 was added to spinach puree and that puree when was added in the conical flask containing hydrogen peroxide which then reacted and produced oxygen which was see by the water displaced by mean of 6.6 ml

Introduction: Enzymes are specifically folded proteins which catalyse biological reactions in living organisms, they consist of an active site in which specific substrates can enter and a reaction takes place. As the enzyme does not permanently change structure, meaning the active site stays the same it allows multiple substrates to complete the reaction through single enzymes. The substrates are complementary to the active site of the enzyme and can be synthesised or degraded in order to create new products useful to the organism. Enzymes enable the substrates to be orientated in a way that allows for the reaction to take place more readily without relying on the chance of accurately positioned collisions of particles with sufficient energy.

In the Enzyme Lab, we observed and quantified the activity of an enzyme. We determined how the concentration of an enzyme affects the rate at which hydrogen H202 deteriorate. When performing the lab, we took paper cutouts, dipped in our enzymes, yeast, and placed them into a container of the hydrogen peroxide. We performed this activity many times so that we could create an average and just in case one procedure failed we would have other to go off of. Once we took multiple tests we compared each of the results to make sure the dilution was correct.

Enzyme is proteins that speed up and regulate the process of chemical reactions within living organisms. Enzyme helps to reduce reaction rates by breaking bonds between atoms (). Biochemical reactions require energy. The minimum amount of energy is necessary to set atoms be able to undergo chemical reactions is called activation energy (Activation energy, 2015). When reactants absorb energy and reach to the activation energy, reactants combine and produce products.