Esophagus cancer (EC) is the 8th most common cancer in the world1, which makes it a high mortality rate cancer. It is estimated that about 17,000 cancer cases will be diagnosed in the USA with 18% of patients having 5-years survival rate2. Adenocarcinoma variants of EC is more common in the USA due to obesity and acid reflux problems3. Moreover, 50% of EC patient develops metastatic EC, which is hard to treat4. To date, very few drug treatments seem to be effective only against locally advanced EC, which implies the necessity of the treatment for metastatic EC5.
Coumarin is a flavoring compound naturally found in plants, which contains a benzopyrone ring. Coumarin and its derivatives (called coumarins) are found in several plants such as
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Besides the vehicle control group, a CDK cell cycle inhibitor and a selective Bax activator will be used as positive controls based on previous literature. MTT assay and CyQUANT direct cell proliferation assay (Thermo Fisher Scientific) will be conducted to measure the cell viability, whereas Scratch assay and Boyden chamber assay will be conducted to measure the cell migration. The most effective coumarin derivative will be selected in terms of inhibiting the cell proliferation and migration, and potencies from in vitro study will be used to determine the dose and the concentration for in vivo study and specific aim 2 respectively. The orthotopic patient-derived xenografted mice model will be used for in vivo study. Tumor volume will be measured using magnetic resonance imaging (MRI) every week for 2 months. After that, mice will be sacrificed and organs (breast, brain, lungs, and stomach) will be tested for any possible metastasis.
Specific aim 2: To investigate the role of coumarin on EC cell cycle and apoptosis. After synchronizing the cell cycle, adenocarcinoma cell line will be treated with various concentration of the coumarin selected from aim 1. Flow cytometry will be performed to determine the stage of the cell division. Bax protein expression will be measured using the western blot method. Moreover, cell viability and cell migration assays will be repeated using a Bax inhibitor to observe the Bax induced apoptotic effect of coumarin.
Stomach cancer, also known as gastric cancer, is a type of cancer that develops slowly over many years. Most frequently, stomach cancer starts in the lining of the stomach otherwise knows as the mucosa, and then develops into the other layers of the stomach. There are many ways in which stomach cancer spreads. In some cases it invades near by organs or it can also travel through the bloodstream to for cancer in other organs. There are many statistics and organizations of support for stomach cancer, as well as, symptoms, risk factors, screening and diagnosis’ and, treatments. Like all cancers, stomach cancer is a deathly disease and one of the major leading causes of cancer deaths in countries other than the U.S.
In vitro: Napabucasin (1 μmol/L) not only decreased cell viability, migration, colony formation, and survival with cell cycle arrest, and increased cell apoptosis and sensitivity to docetaxel, but also obviously blocked PrCSCs sphere formation and killed them in
Esophageal carcinoma, the eighth most common cancer in the world, includes squamous cell carcinoma and adenocarcinoma (Sun, Lin, Chen, Liang, &Hsieh, 2015; American Cancer Society, 2015). Squamous cell carcinoma is simply damaged esophageal cells; however, adenocarcinoma occurs when glandular cells replace squamous cells in the esophagus (American Cancer Society, 2015). While squamous cell carcinoma was once the most common form of esophageal carcinoma in the United States, adenocarcinoma has taken its place with speculation placed towards the increase of gastroesophageal reflux disease (GERD) (Baldwin, 2015). These two manifestations of cancer account for approximately 90% of diagnosed esophageal cancers, while the remaining 10% of esophageal
Treatment for esophageal cancer focuses on removing the cancer through surgery. Chemotherapy and radiation also may be used.
For one, gastric cancer affects many people. In 2011, an estimated 74,035 people in the US had been inflicted with gastric cancer ("SEER Stat Fact," 2013). Additionally, this year, an estimated 22,220 additional people will receive the diagnosis of the stomach cancer ("Stomach (Gastric Cancer)," 2014). Though these numbers may not seem to indicate much of a problem in the United States when compared to prostate cancer's 233,000 estimated new cases and breast cancer's 232,670 estimated new cases, gastric cancer has a significant impact worldwide, being the cancer with the second highest mortality rate ("New Targeted Drug," 2014; "SEER Stat Fact," 2013).
In this research paper, the experimental design was based around the relationship to CCND1 to breast cancer. CCND1 is present during the G1 phase of the mitosis cell cycle. Which occurs right before the S phase. If the CCND1 has a malfunction then the rest of the cell cycle will not finish off correctly into normal functioning cells. The research paper also noted that tumor heterogeneity is a critical issue and there is a hard time treating the tumors because it rejects most of the cell therapy treatments.
Oral cancer is a devastating disease. Over eight thousand American lives are taken by oral cancer and only a fourth of these patients do not excessively drink alcohol or smoke tobacco, which are the two main causes of oral cancer (The Oral Cancer Foundation). Survival rates for oral cancer could be much higher if the population would take precautionary measures to reduce their chances of being diagnosed with oral cancer. When the use of alcohol and tobacco are decreased, the chances of acquiring oral cancer are also decreased. By visiting the dentist at least once every year, new lesions could be found. Unfortunately, only seven percent of the population, who visits the dentist regularly, receives a thorough oral cancer exam(The Oral
As the cancer continues to spread throughout society, many treatments are creating in order to help the patients during their time in need. Although the most popular and used often
In America today finding a cure for cancer is a huge priority with clinical trails being a big part in these goal there are some newly developed drugs and technology that are making a huge impact in the cancer world.
According to WebMD, Lymphatic cancer commonly referred to as lymphoma is the cancer of the lymphocytes. Lymphocytes grow in the lymph nodes, spleen, thymus, and bone marrow. If one has lymphoma, their lymphocytes will begin to grow uncontrollably and change. Lymphoma is a very common type of cancer, that affects many people, however, it is treatable in many ways.
other rapidly growing cells in the body. Chemotherapeutic drugs used to treat cancer are given to most of the people, which help to sustain the completion of cancer treatment (El-Sayyad et al., 2009; Sundaram & Sangavai, 2009). Besides this, the noxious side effects of chemotherapeutic agents include the devastating production of reactive oxygen species (ROS). Therefore, an escalating amount of facts suggest that a simultaneous treatment of chemotherapy and chemo-preventive agents with antioxidant action may augment the efficacy of chemotherapeutics (Lee et al., 2008;Aydin et al., 2011). Although the mechanism underlying the severe cytotoxicities of doxorubicin (DOX) is not fully clear, ROS are assumed to be a key factor in the toxicity of DOX and events controlling this oxidative injury are extensively appreciated (Reddy
Firstly, in hopes of understanding what esophageal cancer is, one must first understand what the esophagus is. The esophagus is a muscular tube connecting your throat to your stomach. It’s primary purpose it to transport food to the stomach and is usually between 10 – 13 inches long. Furthermore, the esophagus is lined with smooth muscle tissue, which causes contracts without conscious thought, moving the food along to the
Cancer has been a disease since the dawn of man and even since that moment, we as humans have strived to understand its’ lethality and discover ways to combat it. This proves to be a more difficult task than it seems. Cancer, by definition, is the uncontrolled division of abnormal cells in the body [1]. These divisions can spread into the surrounding tissues as well and cause damage to the regions they locate to. Many of these divisions can lead to solid growths in the body. The growths are usually referred to as tumors [1]. These tumors are masses of tissue that group together.
HER2 ASSESSMENT IN LOCALLY ADVANCED GASTRIC CANCER:COMPARING THE RESULTS OBTAINED WITH THE USE OF TWO PRIMARY TUMOUR BLOCKS VERSUS THOSE OBTAINED WITH THE USE OF ALL PRIMARY TUMOUR BLOCKS
Gastric cancer, GC, is the fourth most common cancer and the second most common cause of cancer death worldwide; when it is diagnosed it often is considered inoperable advanced or a metastatic disease requiring palliative treatment (Trastuzumab). There is currently no one accepted standard for care, however, fluoropyrimidine-based and platinum-based combinations as well as a third drug, usually docetaxel or epirubicin, is the most used in Europe and the United States. Even tough combination therapies have been reported to benefit those with GC, the actual prognosis for GC remains very poor and new treatments are needed (Trastuzumab). HER2 also referred to as ERBB2, is an epidermal growth factor receptor that has growing evidence