Rosemary On Cold Smoked Sardine

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for

The first result of importance was the result of the Gram stain. The observations of the unknown bacteria from the slant culture after Gram staining showed that the unknown bacteria were Gram negative bacilli (Image 1). After determining the unknown bacteria was Gram negative, an oxidase test was conducted on a sample from the slant culture. The cotton swap with the sample of bacteria did not change color when the oxidase reagent was applied, thus providing a negative result. With a negative oxidase test, further tests were conducted to determine various characteristics of the unknown bacteria. A MR-VP broth was inoculated with a sample from a slant culture of unknown bacteria. After incubation, the methyl red reagent was added to the broth, and the broth turned red, providing a positive result (Image 2). An EMB agar streak plate was inoculated with a sample from a slant culture of the unknown bacteria, and after incubation, growth was found on the plate, providing a positive result (Image 3). A Citrate agar slant was inoculated, and after incubation, growth was found on the media, providing a positive result (Image 4). A Urea agar slant was inoculated, and after incubation, the agar had changed from a peach color to a bright pink color, providing a positive result (Image 5). Using the flowchart (Figure 1) developed from the Table of Expected Results, the lab partners started at the oxidase test. Given the negative result of the oxidase test, the flowchart is

In a laboratory setting, it often becomes necessary to identify an unknown organism. In this experiment, researchers classified an unidentified bacterium based on its physical structure, colony morphology, optimal conditions and metabolic properties. A Gram stain using crystal violet, iodine, and safranin and a simple stain using methylene blue characterized the organism’s cell wall. Cultural behavior was classified by inoculating the organism onto nutrient agar and incubating it at 37° C for 48 hours, and observing its behavior, as well as using SIM medium to test for motility. Optimal growth temperature was

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria can also be used in many other areas from better crop production through microbial pesticides to biological warfare. Their uses are endless as are their abilities to evolve and adapt to changing environments. That is why it is so important

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, catalase, lactose and sucrose fermentation, Kugler/iron agar, nitrate reduction, gelatin hydrolysis, starch hydrolysis, manitol salt, MR-VP, citrate, bile esculin,

The ten most abundant bacterial families detected in our study were Koribacteraceae (Acidobacteria), Acidobacteriaceae (Acidobacteria), Sphingobacteriaceae (Bacteroidetes), Geobacteraceae (proteobacteria), Auto67-4W (Pedosphaerae), Acetobacteraceae (proteobacteria),

During the purification section of this lab, the LB/amp/ara agar plate was examined for well-isolated green colonies and the LB/amp plate was observed for white colonies with space between each other. These colonies were circled on the outside of the plates using a marker. Next, two 15 milliliter culture tubes containing 2 milliliters of nutrient growth media were obtained and labeled “+” and “-“. Using a new inoculation tube, the circled colonies from each plate were scooped out and immersed in their respective culture tubes. Once the bacteria was mixed into the solution, the tubes were sealed and placed horizontally into the 32⁰ incubator for 24 hours.

After the trials were completed, a 1:9 bleach to water solution was poured on top of the prepared agar plates. This safety precaution was used to kill the bacteria in preparation of disposal of the agar plates. After the bleach solution was applied to the agar plates, the solution stayed on the agar plates for approximately 5 minutes. The bacteria on the agar plates turned a yellow color after the five minutes and were disposed of. Each safety precaution implemented in this experiment was to ensure the safety of the experimenter, and other

The purpose to this lab was to isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function.

After gaining some knowledge about bacteria, we were giving an investigating bacteria growth lab to do. Our objective was to observe the conditions required for bacteria to grow and to test the effectiveness of substances that may be antibacterial, disinfecting, and or sanitizing. My group and I began our procedure by gathering all the bacteria by swabbing our necks and mouths. After this, we inoculated the culture by rubbing the bacteria on the agar, a nutrient rich gel made from sea kelp, on the bottom side of the container where we grow bacteria, the Petridish. We hoped for the results to come back with little or even no colonies and an immense zone of inhibition around the tiny circle cut out of filter paper covered in toothpaste, Neosporin, and Chlorhexidine Gluconate 4% Solution.

A positive bacterial growth on nutrient agar plate used as control as well as bacteria colony morphology. The EMB was used to select for coliform gram negative bacteria and determine if the bacteria is able to ferment lactose. The Purple color colonies indicated a positive result for strong fermentation of sugars by fecal coliforms. The last plate is the PEA plate, white showed inhibited growth of the bacteria indicating that the unknown bacteria is gram negative.

The sample tube taken from the pond down by the turf field at USM was taken out of the incubator and observed. This sample was taken February 7th at around 2:30 in the afternoon. The results showed the entire 50ml sample mixed with COLISURE being completely purple, meaning it tested positive for Coliforms. What was different was the 44 wells with the pipetted samples in it. 8 of the wells remained yellow, meaning they were negative for Coliforms and the remaining 36 were all shades of purple meaning they were positive for Coliforms. The well plate along with the 50ml tube of the sample taken from the pond near the turf was then put under an ultra violet light, this helped us detect if the sample was positive for Escherichia coli. Under the ultra violet light, the entire tube sample as well as 3 wells in the well plate were glowing, meaning they were positive for Escherichia coli. In comparison to the other samples, this pond was by far the most dangerous for human consumption or swimming in. After this discovery, the most probable number (MPN) of both positive wells as well as wells positive for Escherichia coli were calculated. For the first sample from the pond near the turf field the MPN was calculated by first identifying the total volume dispensed into the wells. This was calculated was using the equation 0.2ml x 44 wells = 8.8ml. Next, the number of cells per

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.

Live Lactobacillus casei Shirota strain, cultured and tested in our laboratory, is added to the tank. The temperature of the tank is then reduced until the contents are at 37°C (body temperature). The solution is allowed to ferment in the tank for 6-9 days or until the numbers of Lactobacillus casei bacteria reach their ideal concentration.