Mindanao State University-Iligan Institute of Technology
College of Science and Mathematics
Department of Biological Sciences
In partial fulfillment of :
(Scientific Paper on Diffusion)
Submitted by :
Diate, Kim P. (B.S.E Gen.Sci.1)
Submitted to :
Olive S. Anies, Ph.D
This scientific paper investigates my hypothesis that "Higher molecular weight produce faster diffusion". Diffusion is a process of equalization which involves movement of molecules from an area of high concentration to an area of low concentration (Biology 101.1). As Thomas Graham (1831-1833) found out on his study that gases of different nature diffuse with each other, it is a challenge as to how true it …show more content…
In performing the proper experimentation, we poured some of the methylene blue substrate in a piece of paper, for ease transport towards the agar plates. We performed it cautiously by not inhaling it, for it is a form of powder. We carefully place the substrate at the center of the agar plates, to ensure that is that there is enough space for diffusion to take place. After the moment we placed the substrate, we carefully placed a transparent ruler under the plate to measure the diameter of the substrate in 0 minutes. We carefully note the time, having a 15-minute interval and record the changes in the diameter of the substrate. We continued it, until we have an hour observation.
After gathering all the data, we solved for the rate of diffusion of each using the formula.
Rate= Final diameter-Initial diameter Time
After finishing the Methylene blue experiment, we followed the same procedure to Potassium Permanganate.
Each group presented the result to the white board, so that we can compare the results. We kept a record of the results and have a short conversation with our group mates in interpreting the results of the conducted experiment.
Towards the end, we throw the agar gel to the trash can, clean the petri dish, tighten the bottles and clean the tables where we performed the experiment.
Tabular presentation on the Result of the Diffusion rate of Methylene Blue and Potassium
Cells and molecules in the environment are constantly moving and changing, for cells to function properly there is a need for equilibrium to be met. The size of the cell and the solution outside of the cell affects the rate of diffusion and osmosis in the cell. Cells are constantly trying to reach an equilibrium with the molecules and substances around it, which is why there are such terms as: hypertonic, hypotonic and isotonic. The procedures allowed testing of whether or not surface area or volume increased diffusion and how different substance control diffusion. Cells are constantly moving to reach equilibrium through diffusion and osmosis.
Cleaning is the first step in reprocessing instruments and it is called decontamination. The cleaning process is all soiled instruments placed in the prewash ultrasonic washer for ten minutes to remove any bacterial spores for instance, blood, tissue, and bone fragments. Then the instruments are sent through a wall washer. The wall washers are designed for the final cleaning of all instruments. Once the washer is finished, it unloads in the
Two variables that affect the rate of diffusion are the MWCO membrane and the solute concentration. Increasing the membrane size and solute concentration will also increase the average diffusion rate. Decreasing the membrane size and solute concentration will reduce diffusion rates and can even prevent all diffusion.
All organisms need to exchange particles such as food, waste, gas and heat with their surroundings. there are two different types of ways in which substances can enter or/and leave a cell. these ways are called Passive and Active. with in these there are different processes for example, in passive process there are diffusion, facilitated diffusion and osmosis and in the active process there is molecule and particle. in this assignment I am going to discuss all of these processes starting with diffusion.
Time) because it had a correlation closest to 1. All three orders were graphed and a linear regression was used to see which graphed order was closest to 1. The order was determined by comparing the concentration and time to the mathematical predictions made using the integrated rate laws. Analyzing each graph and finding each correlation helped determine which graph was closest to 1. The more concentrated a solution is, the higher the absorbance of that solution. This is due to Beer’s Law. The law measures the absorbance of a solution by determining how much light passes through a solution. As the concentration of a solution increases, fewer wavelengths of light are able to pass through the concentrated solution. The absorbance at 60 seconds was 0.573 (Figure 1: Table1). To calculate the concentration (molarity), the Beer’s Law equation was used, Abs = slope(m)+b. Plugging in what is known into the Beer’s Law equation resulted in 0.573 = 3.172e+004 + 0, where the concentration is determined by M = 0.573-0/ 3.172e+004. So, the concentration at 60 seconds using the equation (M = 0.573-0 / 3.172e+004) was 1.824e-5 M. The 1st order graph resulted in k=0.006152 (Figure 1: Graph 1). Other groups also resulted in their decolorization of CV to be the 1st rate
A major determinant of diffusion in a biological system is membrane permeability. Small, uncharged molecules pass through cellular membranes easily, while most and/or charged molecules cannot pass through the membrane. The movement of water across a selectively permeable membrane, like the plasma membrane
The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.
10.Clean up: Rinse reaction vessel, empty collection box, dry table as needed, cleanup up any baking soda spills..
In conclusion, the potato cube with the highest surface area to volume ratio (the 1x1x1 cube) had the fastest rate of diffusion as it had the largest percentage increase in mass. While all the other cubes of potato had larger increases in mass at face value compared to the smallest cube, the smallest cube had the largest overall gain in percentage. The results support my hypothesis that the smallest cube will have a higher rate of osmosis because it has a proportionally larger amount of surface area compared to its volume.
Diffusion is the spontaneous movement of particles from where they are more concentrated to where they are less concentrated. For this experiment, a 14 cm glass was filled with cold tap water. A drop of red food coloring was dropped in the cup. A stopwatch was used to measure the time it took for the food coloring to get to the bottom of the cup. The average diffusion rate was .78 cm a second. If a different color was used, I do not think it would have made a difference in the results. The mood of the person experimenting could possibly alter the results. For example, if a person is under stress, they may accidentally squeeze too much altering the rate of diffusion. If the water was warmer, this may have sped the