Fabrication And Characterization Of Scaffold From Keratin / Alginate Blend For Tissue Engineering Application

1906 Words Feb 3rd, 2015 8 Pages
Fabrication and characterization of scaffold from Keratin/Alginate blend for tissue engineering application

3. Result and Discussion:
3.1. Compatibility and structural analysis:
The miscibility property of keratin and alginate was studied by applying X-ray diffractometer and Fourier Transform Infrared Spectroscopy. The XRD spectra of blends were compared to the control spectra of native keratin and native alginate as mentioned in Figure 1. In XRD spectra, the native keratin and native alginate give a highest intensity at near 7543 and 4100 at about 19° and 14° angle of 2θ, respectively [17–19]. The spectra of keratin/alginate blends prepared at a ratio of 10:90, 30:70 and 50:50 gives a highest intensity peak 3573, 5128 and 3264 at 22°, 6.8° and 7.1° angle of 2θ respectively. The XRD spectra of all blend ratios gives a new peak in between 6°-8° angle of 2θ which is not present in the spectra of native keratin and alginate, this depict that the structure of native keratin and alginate become altered and form a new structural pattern in blend form. The XRD spectra of blend scaffold revealed that the intensity of blend has transformed as compared to the control XRD spectra which confirm that the compatibility exists in between keratin and alginate. Several works are reported in which compatibility between blend components was examined by XRD. For example, Tian et al. [20] performed the XRD study to identify the interaction between soy-protein and agar. The compatibility between…

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