The Effect of the Light Intensity on the Rate of Photosynthesis
Introduction:
There are many factors that effect the rate of photosynthesis in plants. Some of the factors that impact photosynthesis is temperature, amount of sodium bicarbonate, water temperature, the size of the plant that undergoes photosynthesis, and light intensity. This lab will concern light intensity. The lab will address the outcome of having a really high light intensity compared to a really low light intensity during photosynthesis. Light intensity is defined as the amount of energy hitting an area, or the amount of light an object receives. In this experiment, the object will be baby spinach leaf chads. there will be two light sources in this lab. The light
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Lumens are used to measure light intensity, so the higher number of lumens means the higher the intensity will be. The leaf chads will be placed in bicarbonate water, 10 in each beaker. There will be a total of two beakers. Both of the beakers should be placed the same distance from the light source that they are assigned to for more accurate results. The objective of this lab is to compare the rate of photosynthesis in the leaf chads by looking at the amount of light intensity. The leaf chads that are placed under the light source labeled (b) will have a greater rate of photosynthesis compared to the leaf chads that are placed beneath light source (a) because that leaf chads under light source (b) will receive more photons (energy packers) to carry out photosynthesis. During photosynthesis, the energy of light captured by pigment molecules, called chlorophyll, in chloroplasts is used to generate high-energy electrons with great reducing potential. In electrons are used to produce NADPH as well as ATP in a series of reactions called the light reactions because they require light. That is why light intensity plays a really important …show more content…
Place 10 leaf disks into the syringe and pull in a small volume of the bicarbonate solution. Replace the plunger and push out most of the air, but do not crush the leaves.
Create a vacuum by covering the tip of the syringe with your finger. Draw back on the plunger. Release the vacuum so that the solution will enter the chads. It might take a few times to get the disks to sink. You may need to lightly tap the syringe to dislodge the chads from the sides. Once they have sank, put them back into the bicarbonate solution and place the chads under the light source that measures 450 lumens. Start a timer and record how many of the chads are floating at 1 minute intervals.
Repeat steps 2-5 for the other 10 leaf chads, but place them under the light source that has the measure of 800 lumens.
Materials:
2 beaker half filled with Bicarbonate solution
Baby spinach
800 lumen
Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.
30. Same as carried out in Part (b), repeat steps 18. to 24. to dissolve the gas in the syringe in the heated de-ionised water.
Introduction: Photosynthesis can be defined as a solar powered process that removes atmospheric carbon dioxide and transforms it into oxygen and carbohydrates (Harris-Haller 2014). Photosynthesis can be considered to be the most important biochemical process on Earth because it helps plants to grow its roots, leaves, and fruits, and plants serve as autotrophs which are crucial to the food chain on earth. Several factors determine the process of photosynthesis. Light is one these factors and is the main subject of this experiment. The intensity of light is a property of light that is important for photosynthesis to occur. Brighter light causes more light to touch the surface of the plant which increases the rate of photosynthesis (Speer 1997). This is why there is a tendency of higher rates of photosynthesis in climates with a lot of sunlight than areas that primarily do not get as much sunlight. Light wavelength is also a property of
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
The purpose of this experiment was to investigate the effects of light intensity on the rate of photosynthesis in a Moneywort plant. By observing the plant in distilled water mixed with sodium bicarbonate, different light bulbs were targeted onto the plant. The measurement of the amount of bubbles present on the plant during the trial of the experiment enabled us to identify the comparisons between the activity of the light and the process of photosynthesis.
12. Describe two ways (two different things you could measure) to estimate the rate of photosynthesis in a particular plant. What specific measurements would you need to make for each in order to estimate GROSS photosynthesis?
Pick up the dropper and put it in the cup (filled with sodium chloride) and squeeze the top of it to pick up the liquid.
The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less
The intention of this experiment is to determine the effects of pH on the rate of photosynthesis in living leaves. Photosynthesis is a process by which plants convert light energy captured from the sun into chemical energy which they use to perform various plant functions. During the photosynthesis process, light, carbon dioxide, and water react to produce products: sugar and oxygen. The equation for photosynthesis is:
Begin by inserting a cheesecloth into a syringe and pressing the cheesecloth to the very bottom of the column.
Submerge the graduated cylinder in the plastic tub so that it is completely filled with water. Hold the open end of the graduated cylinder and move it vertically upside-down where the open end of the graduated cylinder is still submerged in the plastic tub. Clamp the graduated cylinder the ring stand of the lab table to keep it in place. perforate a hole in the top of the rubber cork for the solution container. Cut a straw the length of about four inches. place the straw inside of the rubber cork hole. Set up your timer for two minutes.
Different distances were used in this investigation in order to answer this question: Does distance from the light source affect the rate of photosynthesis? Our hypothesis states that: Different distance from the light source affects the rate of photosynthesis. And our prediction is that the farther away the light source is the longer it takes for the process to occur, and vice versa; the closer the light source is, the faster it takes for the process to occur. After doing this lab we can conclude that distance from the light source affect the rate of photosynthesis. Because in our conducted experiment, different floating times were associated with different distances from the light source.
Because chlorophyll-a takes in violet-blue, and orange-red lights to use in photosynthesis, it reflects green, and that is the color we see. This study investigates the relationship between the wavelength of light and the total respiration of the plant Elodea. The purpose is to see the effect of the presence or absence of certain wavelengths can have on the process of photosynthesis. Because Elodea is a plant that reflects green light, it can be assumed that red lights, the complimentary color to green will be absorbed the most contributing to the process of photosynthesis and respiration making the most oxygen.
Plant- Different species plants have different photosynthetic rates due to the different leaf structures of the plants. Even plants of the same species may have slightly different rates of photosynthesis since there may be more or less chlorophyll in the leaves to absorb light. The size of the plant is also important since this would affect the amount of surface area for gas exchange.
The second hypothesis on the rate of photosynthesis (green/white light) was correct because the data in the table above states that the rate of photosynthesis (which was measured in volumeters) was higher in the tube that was exposed to white light.