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How The Amount Of Motor Oil Affects Hatching Viability Of Brine Shrimp Eggs Essay

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AP Biology Lab Report: How the Amount of Motor Oil Affects Hatching Viability of Brine Shrimp Eggs

Question
How does the amount of motor oil in the environment of a brine shrimp affect the hatching viability of brine shrimp eggs?

Hypothesis
If hatching viability is affected by the amount of motor oil in the environment of the brine shrimp eggs, then when the amount of motor oil is increased the hatching viability will decrease.

Experimental Design
The independent variable was the amount of motor oil in 2% salt solution because it is the variable that was being tested and purposefully manipulated. The dependent variable was the hatching viability of the brine shrimp tested (which was calculated by adding the number of brine shrimp swimming at 24 hours to the number of brine shrimp swimming at 48 hours, and then divided by the total number of eggs initially placed in the petri dish). The control group was a petri dish of brine shrimp in 30 ml of 2% salt solution with no motor oil. The constants, or variables that need to be controlled, were the salinity percentage of the salt solution (2%), the amount of salt solution in each petri dish (30 ml), the total time the brine shrimp were left in the petri dishes (48 hours), and the frequency of when the brine shrimp were checked (every 24 hours).

Procedure
Materials:
Approximately 100 brine shrimp eggs
Scissors
Pipet
Paintbrush
5 microscope slides
1 stereomicroscope
5 pieces of double-sided tape
5 petri dishes
Permanent marker

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