AP Biology Lab Report: How the Amount of Motor Oil Affects Hatching Viability of Brine Shrimp Eggs
Question
How does the amount of motor oil in the environment of a brine shrimp affect the hatching viability of brine shrimp eggs?
Hypothesis
If hatching viability is affected by the amount of motor oil in the environment of the brine shrimp eggs, then when the amount of motor oil is increased the hatching viability will decrease.
Experimental Design
The independent variable was the amount of motor oil in 2% salt solution because it is the variable that was being tested and purposefully manipulated. The dependent variable was the hatching viability of the brine shrimp tested (which was calculated by adding the number of brine shrimp swimming at 24 hours to the number of brine shrimp swimming at 48 hours, and then divided by the total number of eggs initially placed in the petri dish). The control group was a petri dish of brine shrimp in 30 ml of 2% salt solution with no motor oil. The constants, or variables that need to be controlled, were the salinity percentage of the salt solution (2%), the amount of salt solution in each petri dish (30 ml), the total time the brine shrimp were left in the petri dishes (48 hours), and the frequency of when the brine shrimp were checked (every 24 hours).
Procedure
Materials:
Approximately 100 brine shrimp eggs
Scissors
Pipet
Paintbrush
5 microscope slides
1 stereomicroscope
5 pieces of double-sided tape
5 petri dishes
Permanent marker
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The article "The Amazing Penguin Rescue" main idea is that oil spills can cause lots of problems to the animals such as the African Penguin. For example on paragraph 7 from "The Amazing Penguin Rescue" it states "You are not the only penguin that has become soaked with poisonous oil." Another example is found on paragraph 13 it states "It
The purpose of this lab was to answer the question, “Of the saline solutions of 0%, 0.5%, 1%, 1.5%, and 2% sodium chloride (NaCl), which solution will yield the highest hatching viability for the brine shrimp?” The hypothesis was that the saline solution most precise to the concentration of NaCl in seawater, which is approximately 3.5%, will yield the highest hatching viability. Therefore, the 2% NaCl solution will yield the highest hatching viability.
4. The Independent variable is the dissolved oxygen and the dependent variable is the number of fish observed.
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The development of A. salina will be unfavorable if exposed to too much ethyl alcohol. Treatment two and three have more ethanol alcohol than treatment one and more brine shrimp died when put into more ethanol alcohol. Since the viability was less than the viability in treatment one, the hypothesis was supported. Treatment four was the constant and had no ethanol alcohol which less cysts died when compared to treatment two and three, but when compared to treatment one, more had died,
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One of the most noticeable, and troubling, effects is on shorebirds. The coast guard has / had recovered 329 oiled birds from Galveston Bay to North Padre Island, but nearly all dead, and observations of at least 500 birds with some traces of oil on Mustang island. Mant birds come across the oil as it washes ashore, and ingest the oil by preening. This has a number of fatal consequences. All the poor mammals in the ocean such as fish, marine animals, and those involved in the area's multi-billion dollar commercial and recreational fishing industry. Galvestons Bay's fishing industry has a high Possibility of being hurt by the spill. Studies found that some fish embryos developed deformities caused from the 2010 BP Oil Spill, and many concerns are that this spill will have the same similar effects. Soon as time walked along In late March, 50 local businesses filed a class action lawsuit in federal court against the two companies whose boats were involved in creating the
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