AN INVESTIGATION INTO THE EFFECTS OF SALINITY ON THE HATCHING OF BRINE SHRIMP
WRITTEN BY BRONTE FLYNN
Abstract
Brine Shrimp are a micro-crustacean that is found mostly in inland saltwater bodies, however, the salinity levels that they live in vary greatly. The conditions that they require can be made at home as the eggs (cysts) of the shrimp are dry, hard shells that can withstand drought and go without water for up to 50 years. This study presents the results of 3 different salinity levelled environments to the Brine shrimp in order to discover a salinity level that produces the most successful hatching rate. By filling 3 Petri dishes with 20mL’s of purified water, each dish contained varying sodium chloride amounts from 15ppt, 25ppt, and
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Taking about a week to fully mature into an adult and living for several months, the shrimp do have a surprisingly long life cycle being also able to reproduce up to 300 cysts every 4 days in the right conditions. Other variables such as salinity, temperature, light, pH levels, water levels and predatory presence play a large role in the hatching rate of the crustacean. The brine shrimp are an extremely versatile micro-crustacean as they can survive up to 10x salt concentration of ordinary sea water (Eol.org, 2016) and are able to protect themselves during drought or when conditions are not ideal for survival. This is called highly resistant encysted gastrula embryo (cyst) that is easily able to hatch under conditions that can be replicated at home.
There are numerous variables that impact hatching rate such as the salinity levels of the habitat as the cyst would not hatch if the salinity was not ideal. If the conditions were not correct, the cysts remain dried and surrounded by a thick shell until ready to develop which can be up to 50 years. Once the conditions are met, the cyst takes approximately 36 hours until the shrimp is released; from there, the adolescent can produce up to 500 nauplii. The salt concentration, when too high, impacts hatching as osmosis cannot occur correctly. The water outside the shell would have a higher
If hatching viability is affected by the amount of motor oil in the environment of the brine shrimp eggs, then when the amount of motor oil is increased the hatching viability will decrease.
Firstly adult female brine shrimp ovulate approximately every 140 hours and in optimal conditions the fertilised eggs can almost immediately hatch, even over a relatively short lifetime of a year this is a lot of young. They have another brilliant reproductive adaption; while in extreme conditions, such as low oxygen level or salinity above 15‰, female brine shrimp produce eggs with a chorion coating which has a brown colour. These eggs, also known as cysts, are metabolically inactive and can remain in total stasis for two years while in dry oxygen-free conditions, even at temperatures below freezing. The shrimp have also been able to survive dangerous salinity levels as the eggs wait to hatch if the salinity levels are too high. Once placed back in briny water the eggs hatch within a few hours. This ability also has enabled them to spread the population by the eggs travelling on the feet of birds. They have also been known to reproduce asexually by parthenogenesis in the absence of
One of the limitation was that the experiment was unable to simulate the effects of predation. In an actual environment some of the brine shrimp eggs could have been eaten before they are able to hatch, but since this was in a petri dish there was no such thing as predators that have reduced the hatching viability of the brine shrimp eggs. Another, limitation was the exact amount of brine shrimps you could brush up. This made the hatching viability to not be as accurate because there was different amount of eggs to start with. If there was an equal amount of eggs to begin with the hatching viability percentage would have been more accurate since they all started the same. These two limitations made the experiment not as reasonable because it stopped what could have happen to these brine shrimps in their actual
Shark mating rituals vary from the size of the shark and its specie. In smaller, more flexible species, the male coils around the female, while the male of the larger specie orients himself parallel and head-to-head with the female (Woums & Demski 1993). Some species mating rituals also consists of males biting females on the pectoral fins or the middle of the back to hold on to them (Woums & Demski 1993). The female will often bear scars or marks, after the ordeal. Due to the fact that sharks are cold blooded animals, there is no precise gestation time. The development of the embryo will depend solely on the temperature of the water, however, the gestation period can last anywhere from 2 months to 2 years (Woums & Demski 1993). Within shark species there are two methods of reproduction. In some cases a female shark will lay eggs in a coastal nursery where the presence of other sharks are absent. And in other cases, usually in the larger sharks, females give birth to live offspring (Wourms & Demski 1993). In which case the young are born fully developed, and once they are born they shoot away fast or they risk being eaten.
To approach this problem, eggs of the brine shrimp were placed in 5 different solutions with different salinity. By observing how many eggs hatch per given number of eggs in the solution, the hatching viability was calculated for each solution.
Brine Shrimp start out at something called cysts which you can buy all over the world and it takes only one night in water for them to hatch into the Brine Shrimp. At a maximum length, Brine Shrimp only get up to a little over 1 centimeter which is perfect size for fish around 10 centimeters. I thought that growing the Brine Shrimp was one of the most interesting things that I did while I was there.
Behavior of Brine Shrimp in Habitat Selection Introduction: This experiment was developed primarily to find the optimal or preferred living conditions of adult brine shrimp. In this lab 3 variables were tested in different degrees to determine which the shrimp prefer. The different variables were light, ph, and temperature. From outside resources I have found that brine shrimp can survive in temperatures ranging from 4° Celsius to 40° Celsius with an optimal temperature of 20°-25°. So I hope to reinforce this research by testing its validity in the lab. From another outside source brine shrimp display positive photo taxis, which means that they are attracted to light. And finally from my last outside source I have found information that
Therefore this experiment was to determine that lobsters in various salinities will osmoconform to their environment. In order to test that lobster's osmoconform, we had to extract approximately 1.0 ml hemolymph from their hemocyannin on the ventral first section of the pre-branchial region. The hemolymph was spun for three minutes in a microcentrifuge and the serum was then tested on an osmometer, which determined the osmolarity of the hemolymph. The results substantiated the hypothesis, in that, lobsters internal osmoles fluctuate with the salinity of the external environment. The two lobsters in the low salinity tank had the lowest osmolarity 0.746 osmoles; the two lobsters in the normal salinity had 0.873 osmoles. The last tank with the highest salinity had the lobsters with the highest osmolarity at 1.445 osmoles. Therefore our data suggests that lobster's osmoconform, with respect to the salinity of their environment by readjusting their intracellular solute concentration to prevent swelling or dehydration because the osmolarity of their hemolymph dictates that of the environment.
This experiment was performed using the crustacean Armadillidium vulgare, also known as the pillbug. Pillbugs mate in the spring, and it takes several weeks for their fertilized eggs to hatch. After hatching the offspring spend an additional six weeks in a pouch under the female pillbug. Pillbugs eat dead and decaying organic matter, so they are often found in dark damp places. Another reason they like damp places is because they have gills that must be kept moist. The gills are located on the thorax, which is covered by large exoskeleton plates. The thorax is also where the pillbug’s seven pairs of legs are located. The pillbug also has the nickname “roly-poly,” this is because the pillbug has a defensive mechanism where it rolls into a tight ball, using the large exoskeleton plates of its thorax to protect its head and underside.
Dominus in Litore has an estimated lifespan of 30 years, at the age of 1 they are almost half their full grown size! The females can lay as many as fifty eggs. Sadly, when the eggs are barely laid, their shells are so soft that freshly fallen snow can break it. After five days, the shell can turn unbreakable, a diamond can try to scratch it but will break it at the same time. These facts might be helpful when you encounter
The development of A. salina will be unfavorable if exposed to too much ethyl alcohol. Treatment two and three have more ethanol alcohol than treatment one and more brine shrimp died when put into more ethanol alcohol. Since the viability was less than the viability in treatment one, the hypothesis was supported. Treatment four was the constant and had no ethanol alcohol which less cysts died when compared to treatment two and three, but when compared to treatment one, more had died,
Our end results only supported to some extent our hypothesis that increasing numbers of brine shrimp would directly cause decreases in algae concentration. Our hypothesis, if applied to only the jars of 3 and 6 brine shrimp, would be highly supported, due to the significant difference in algae concentrations. Evidently, the more shrimp that were present in the ecosystem, the less algae that were available at the end. In an ecosystem with more consumers, the consumers demand a higher amount of nutrients from the environment, or more specifically, the producers. And as the consumers develop and grow, those demands also grow. And thus, when we increased the amount of brine shrimp, more algae were consumed per unit time. Though the rate of consumption from the brine shrimp did not exceed the rate of growth of the algae in any jar, the
Without oyster reefs, animal habitats are lost because the current is pushing against the shore, endangering the animals. Oyster reefs allow marsh grass to grow. Oyster reefs also provide habitats for oysters, shrimp, and crabs. Oyster castles help prevent shore erosion and water clarity. In addition, oysters exist here in the Lynnhaven River for many reasons. Oysters can SURVIVE in 5-35 ppt( parts per thousand), but they especially thrive in 12-25 ppt. Salinity levels also impact the presence of diseases. For example, at 10 ppt oysters can get diseases such as MSX or Dermo. If the salinity is below 10 ppt for 10 or more days, MSX will be eliminated. In places with lower salinity,around 12 ppt, oysters grow slower. In places with higher salinity, around 25 ppt, oysters grow faster. Their growth can also be affected by tempreature, food, and disease. The Lynnhaven River has high salinity, lots of food, and minimal diseases in most locations,ideal conditions for an oyster. Equally important is the roles other animals play to oysters and their ecosystem. Bioturbators are animals that dig up sediments and particles while making their burrows. Mud crabs are dominant
After 3 days in the diluted seawater the crab’s haemolymph Sodium levels were significantly lower (p<0.001) than the crabs stored in full strength seawater as seen in figure 3. There was no significant difference between the mean haemolymph Sodium values of the three dilutions at 75%, 50% and 25%.
Larvae are very tolerant to low nutrient sources, mainly feeding of organic particulate matter in the water. They go through 4 instars after which they turn into pupae. They do not eat during this stage and take 2 days to develop into adult mosquitos.