INTRODUCTION In the past, and even in modern times like today, it has been vital to distinguish and determine the identities of microorganisms in the world. These identities are not only important in knowing what agent causes various diseases and the treatment to be used, but also in understanding how microbes can be beneficial and valuable to the human body and life as a whole. With that being said, upon beginning this lab, the purpose of this study was to identity and investigate an unknown microorganism by applying the methods that were previously learned and practiced in the microbiology laboratory portion of class.
METHODS AND MATERIALS Upon beginning the unknown lab, I was given the opportunity to choose an unknown broth tube. This tube contained our unknown microorganism and it was my job, based upon the testing methods learned throughout the semester to distinguish which microbe I had chosen. The goal at this point was to create a reserve plate and a working. This was completed by inoculating one TSA plate thoroughly in order to secure a lot of the growth. This plate became my reserve plate. The working plate was created by using the Streak Method that was introduced at the beginning of the semester. With this method, I would soon be able to visualize and record my microbial growth and colony characteristics. These plates were incubated for a week as that is the time span between classes. When returning the class the following week, I obtained both my
There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation
You can grow microorganisms in liquid media or on solid media. Most bacteria can be grown in labs as long as the media contain a source of the major nutrients; carbon, nitrogen, sulphur, and phosphorous. They may also need to have other nutrients. These nutrients are made into a broth and the pH and salinity can be adjusted. The nutrient broth is placed in test tubes, which are plugged with cotton wool, capped with foil and then sterilised in an autoclave, at 121c for 20minutes. These tubes are then cooled before they are inoculated. To prepare the streak plates we dipped an inoculating loop into ethanol then placed it in the flame until the loop glowed red. Still holding the inoculating loop by its handle we removed the lid from
Unknown lab report# 24 Introduction Microbiology is a study of organisms that surrounds us every day. It requires an educational understanding to identify organisms, and the ability to distinguish different bacteria’s. In applying the learning process of the different bacteria’s, unknown bacteria samples are given to be studied and identified.
There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.
A wide variety of processes had been performed to determine what culture had been acquired in class. My group had acquired culture tube unknown #3. We first isolated the bacteria. In this step we took the broth of the unknown #3 and grew it on an agar plate. The first process that had been performed was discontinuous streaking, and continuous streaking to grow the culture for future use in the lab and to have extra to perform a whole variety of test to determine the unknown. The next experiment that had been performed
In class, we were given the task of identifying an unknown bacterium broth culture. After receiving number 69, I went through several tests to figure out what bacterium I received. First, I created a slide from my broth by putting a small amount of the unknown broth on to a clean slide and letting it dry for ten minutes. After this, I stained the slide by applying four reagents in order; crystal violet, grams iodine, decolorizer and safranin. From the stained slide, I discovered that this bacterium was gram-negative, which would determine the next couple of tests I would do to identify my unknown bacterium. I began by streaking for confluent growth from my broth culture onto a TSA plate. From the TSA plate, I aseptically transferred a loop
This project’s purpose as a whole was to receive an unknown bacteria and figure out what it is; One can figure this out by doing a series of tests. These tests include but are not limited to: gram stains, capsular stains, MacConkey agar plates, SIM, KIA, and UREA tubes, Catalase and Oxidase tests, Methyl Red and Voges-Proskauer test, and many, many more. Although that was just naming some of the tests one can do, what they are and how they’re done are further explained in the methods and reports section. Although there were many tests I was able to do, I was limited to an extent. I only had a few weeks to work on the project, and there were some unavailable tests I was unable to do.
The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible
This laboratory experiment’s objective was to take a pure culture and isolate it from a mixed culture. The other part of the objective was to ascertain what species of bacteria that the pure culture was. The hypothesis made stated that so long as lab protocol was followed, the unidentified culture would be positively recognized/identified. An isolated pure colony of the unknown culture was obtained using the quadrant streak plate method. Afterward, the culture was Gram stained, and the results showed that it was Gram positive. Motility tests were done on the unknown using a filter paper bridge on a petri dish that contained TTC with agar. The unknown was revealed to not be motile, which meant that it did not possess flagella. The last test done was to learn the metabolic capabilities of the unknown bacteria. There were tests done for citrate utilization, the mixed fermentation pathway, catalase presence, carbohydrate fermentation in mannitol, lactose and glucose, urease production and the butanediol fermentation pathway in order to better identify the unknown bacteria. The results from each of the metabolic tests in conjunction with the motility and Gram staining tests were ultimately compared to results from database containing many different kinds of results from various bacteria. The unknown from the mixed culture was identified as Staphylococcus
The purpose of this study is to identify an unknown bacterium from a mixed culture. We identified the unknown bacteria through different biochemical tests and identified the many metabolic capabilities of the bacteria. Studying and discovering unknown bacteria is very important in the health field as it may lead to new medicines and innovations for current, existing diseases as well as any upcoming diseases. The discovery of unknown bacteria is very useful in the pharmaceutical industry, as most of the products prepared are made based on these unknown organisms.
The main objective of this lab was to identify different bacteria by simple, negative, and gram staining. To view each bacteria cell, the bacteria was transferred aseptically to a slide, and they were then viewed by using oil immersion, by a light microscope. From this lab, it was determined that E. coli and B. megaterium are gram negative and B. subtilis and S. Marcesans are gram positive.
The purpose of this lab was to identify two unknown bacteria from a mixed culture. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics. This is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Also knowledge of different bacteria helped others make antibiotics used today. This lab was completed by using the methods learned thus far in identification of bacteria.
These isolated bacteria were identified using both morphological culture characteristics (i.e. the color, shape, elevation, capacity, consistency, edge) and biochemical test (i.e. citrate, oxidase, indole, sugar fermentation, test etc.)and the bacteria were identified based on the results obtained from the above mentioned biochemical characterization results and the procedures include.
The identification of bacteria is a crucial procedure in the field of biology. Identification can be done through a series of tests run on different forms of media that detect the physical and biochemical properties of the cells. In this experiment, a sample containing two unknown species of bacteria was run through fourteen tests to determine morphology, fermentation properties, reducing and oxidative properties, and other various characteristics of each isolated unknown. It was concluded that the sample contained Lactococcus lactis and Shigella flexneri.
Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a