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Kidney Function Lab Report

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Human Kidney Function
Renee King
TA: Oliver
Biology 3U03
November 24, 2016

Treatment with water, caffeine, alcohol, and gatorade and the effects on urine output, pH, Na+ excretion, and solid excretion were tested at 30 minute intervals for 2 hours. It was found that urine production for each treatment group increased after 30 minutes, increased further after 60 minutes, and then began to decrease. Mean increases in urine production were only significant for Gatorade in the first 90 minutes (an increase of 40% at 30-60 minutes, and a decrease of 73% at 60-90 minutes) and for caffeine over 60-90min (a 48% decrease). The average pH for each treatment group became more acidic by 90-120 minutes. Mean differences over each time …show more content…

A decrease in solute concentration caused by consuming water inhibits ADH and causes the distal tubule and collecting duct to become impermeable to sodium and causes the concentration to increase in the urine (VanPutte et al., 2014). Once the volume load is excreted (327.01mg/kg of sodium), sodium concentration becomes normalized and decreases. Caffeine inhibits the reabsorption of sodium by inhibiting ADH, thereby stimulating an increased Na+ and water excretion, as water is drawn into the tubule due to the high Na+ concentration along its osmotic gradient. Since alcohol also inhibits ADH, it will also cause Na+ to be excreted. The two follow a very similar trend, but the mean differences in sodium excretion are not significant. A study done by Maughan et al. (1994) showed that urine osmolality was highest in individuals over the first hour after consumption, and then began to fall as the body begins to restore water and electrolyte balance. These drinks cause a direct consumption of electrolytes. This is reflective of the pattern seen in Gatorade treatment groups, where Na+ concentrations increased by 82% by 60-90 minutes and then began to drop. The significant difference in the total sodium excreted by the water treatment groups may be due to the small consumption of each of the other three treatments, as mentioned above, which don’t have as large of an affect unless consumed in …show more content…

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