Diffusion and Osmosis Experiment with a Shell-Less Egg After Three days of Testing Methods with Water and Corn Syrup
Lisa
July 1, 2013
Purpose
To use the properties of diffusion and osmosis to see the effects of either corn syrup or water on a shell-less raw egg over a three day period. While looking to see the effects of these liquids on the raw egg, one can also apply the properties of hypotonic, hypertonic, and isotonic solutions.
Introduction
Cells have an outer covering called the cell membrane. The membrane is selectively permeable meaning that it has tiny pores or holes that allow objects to move across it. The cell membrane controls what moves in and out of the cell. Food and oxygen move into cells across the cell membrane through
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The egg after its treatment from the corn syrup was then weighed on the scale once again and its mass was recorded as M2.
Addition of the Water
This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.
Results
Table 1.1- Mass of Egg after 3 days of testing | Egg #1 (in grams) | M1 (after Vinegar) | 86.2 grams | M2 (after Corn Syrup) | 51.3 grams | M3 (after Water) | 96.3 grams |
Table 1.1 shows that the egg increased in size (in grams) from 86.2 grams to 96.3 grams.
Picture 1.1 -Egg after 24 hours in Vinegar
Picture 1.1 shows the egg after it had been soaked in vinegar for 24 hours. The vinegar solution become very bubbly and had a “frothy” texture that covered the egg.
Picture 1.2- Egg after the shell was removed
Picture 1.2 shows the raw egg after the shell had been
In the rubber egg lab also known as the osmosis lab, I first measured the circumference of the egg which was 6 inches. The egg had no cracks and was hard. I placed the egg in the vinegar and within seconds the egg started to bubble. These bubbles were carbon dioxide. After 72 hours the shell of the egg had started to dissolve or flake. This would be considered passive transport as the vinegar diffused across the egg shell without any force. Seventy-two hours into the experiment the membrane was exposed, and had a circumference of 8 inches. Before the egg had a shell and you couldn’t see through it but once the vinegar acted as an acetic acid it broke down the shell and left a yellow membrane that felt like rubber this is an example of diffusion. I then placed the egg in another container and put corn syrup over the egg for 24 hours. The egg had shriveled and shrunk, the water had left the egg and went into the syrup and that is what caused the egg to shrink. The corn syrup is essentially pure sugar with very little water so the osmotic pressure is very low. I then placed the shriveled egg in water and waited for another 24 hours. After observation the egg had no
Osmosis took place in the egg under a variety of conditions because the membrane of the egg has tiny openings where water can pass through from high to low concentrations. On day one, it was a hypotonic solution because the higher concentration of water in the vinegar moved to the smaller concentration in the egg. This means the egg was filled with water, which resulted in the eggs increase in size. On day three, the hypotonic solution occurred because the corn syrup had a lower concentration of water than the egg. That means the egg released water into the corn syrup, which resulted in the eggs decrease in size. On day four, the egg was placed in water which resulted in an isotonic solution where the concentrations become even. This
This exercise is aimed at giving a better understanding of the process of osmosis by analyzing how the decalcified eggs behave under different experimental conditions. The shell-less eggs used represented a model for a living cell and its selectively (semi) permeable membrane.
The osmolarity of a solution is the concentration measured of a solute. To determine the concentration of a solution, one must first understand what osmosis is and how it works. Osmosis is the spontaneous passage or diffusion of water or other solvents through a semipermeable membrane according to the Britannica Encyclopedia. The semipermeable membrane is one that blocks the passages of dissolved substances such as solutes. This process, in more recent times, provides a more accurate study of how water can diffuse across a cell membrane when water molecules have a high concentration to an area in which they have a low concentration. It was originally studied in 1877 by plant physiologist, Wilhelm, Pfeffer. (Britannica Encyclopedia 2015).
What will happen to the egg when a student tests osmosis with 3 different soultions in a cup.The student is trying to see or find the effects of osmosis on an egg.Osmosis is the movement of water molecules through a selecfively permeable membrane. Diffuision is when something spreads more widely and equilibrium means when somethings is at rest due to equal opposite forces. The student wants to find out if the egg will shrink, fill up, or change in any way.
The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.
Though the theory following the hypothesis is correct and the experiment was carried out with as much attention as possible in a high school laboratory, the results obtained were still indicative of a few errors and did not support the predicted hypothesis. From the results obtained it can be concluded that as the concentration of sucrose increases the average percentage change in mass decreases. This is because the salt concentration inside the potato cubes of 10%, 15% and 20% concentration is less that in the salt concentration on the sucrose solution, thus the three cubes submerged in the 10% - 20% concentrated solution lost mass (hypertonic). However the cube submerged
The lid was then placed on the jar and an observation was made and recorded. Observations of the egg were then made after 12 hours and 24 hours had passed and the observations were recorded. After 24 hours had passed, the egg was removed from the syrup and rinsed with tap water and observations were made and recorded.
An egg has a semi-permeable membrane, thus processes like osmosis could occur. Osmosis is the movement of water molecules from an area of higher water concentration to an area of a lower water concentration. Osmosis is important, especially for living organisms, as they help distribute nutrients in the body. An egg’s mass would change when it is soaked in different substances. The goal of this experiment was to investigate how an egg changes through osmosis. This experiment was done to show how substances affect the mass of an egg. To start off, an egg’s mass was measured by putting the egg in a beaker then placing it on a mass scale. The beaker was then filled with vinegar and left alone for twenty-four hours. After a day, the egg was taken
In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which
Osmosis is diffusion of water across a selectively permeable membrane. The net movement of water through the membrane goes from an area of high concentration to an area of low concentration until equilibrium is found. When a cell is placed in an isotonic environment, equal solute concentration, there will have no net movement of water. A hypertonic environment will have a greater solute concentration, where the cell will have a lower solute concentration. A hypotonic environment will have a lower solute concentration and the cell will have a higher solute concentration. A small lab experiment was done, with the purpose of finding the solute concentration, inside decalcified eggs. The experiment was done using four decalcified
The concentration of acetic acid in the vinegar dissolves the calcium carbonate, which the shell is made of. Corn syrup has a lower concentration of water than the egg so osmosis is necessary to make the solutions isotonic. Water has a lower concentration of solutes than inside of the egg, so when the egg was submerged in water the water entered through the egg's membrane until it reached an isotonic
An average cell contains approximately 70% water, which is what I assume this egg’s original water concentration was before any liquids were surrounding it. Every bit you can find out from the chart or graph, when the egg was placed in vinegar, it expanded twenty millimeters. The shell also dissolved, because vinegar is acidic. Although vinegar does not cause high water content, it was still able to go into the testis. Vinegar is made of very small particles, like water, which made it possible to grow inside the egg after dissolving the scale. Too, the concentration of vinegar was higher outside than in, which explains why it
Cells in all living things have an outer layer known as the cell membrane. The structure of the cell membrane consists of the phospholipid bilayer organized by the arrangement of hydrophilic heads and hydrophobic tails. It is a selectively permeable membrane, where it divides the outer environment from the interior of the cell. It can control substances moving in and out of living cells. Certain molecules like gases, water, and food are permitted to pass the membrane through the method of diffusion. Diffusion refers to the process in which molecules move on the concentration gradient, where they move from an area of high concentration to an area of low concentration. A type of diffusion is known as osmosis. It is the diffusion of water moving across the selectively permeable membrane. In this lab, students will be using eggs to construct an experiment to get a better study on how osmosis works in a cell. The eggs will be soaked in vinegar solution to remove their shells to expose each inner layer that resembles a selectively permeable membrane. The egg shell is composed of calcium carbonate that would dissolves in acidic solution such as vinegar. In the chemical reaction, it releases carbon dioxide gas. After the removal of the egg shell, it will be ready to be able to construct the experiment.
4.15. The purpose of this investigation was to use our knowledge on osmosis and diffusion and apply it to a de-shelled egg and see how it reacts being submerged in a sodium chloride solution. The hypothesis was that the egg would expand and increase in both size and weight this is proven correct in the table of