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Lab 12: DNA Transcription, And Protein Synthesis

Decent Essays

Leah Romero
04/25/2018
Lab Report
Chem. 102L

In lab 12, DNA Replication, RNA Transcription, and Protein Synthesis, the main purpose was to be able to understand the structure of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) molecules as the way DNA replication is created. There were several different models done to understand transcription, translation, and protein synthesis. The process of mutation changing to the genetic code was observed and the results of it. The main outcomes looked for in this lab were to be able to describe the basic structure of DNA and RNA, be able to design a simulation model that shows the replication of both RNA and DNA, to be able to create models in a simulation that demonstrate both transcription and …show more content…

The chemical properties of this lab all tie in with the nucleotide order of the DNA molecules. DNA is arranged to form a double helix with two complementary strands made up of nucleotide bases, sugars, and phosphates. RNA is similar to DNA but RNA contains ribose and DNA contains deoxyribose. There are four nucleic acids that are found in DNA and those are adenine, guanine, thymine, and cytosine. In nucleotide, the phosphate group is bound to the 5’ carbon of the sugar molecule and the nitrogenous base is bound to the one carbon of the sugar molecule. The phosphate-sugar backbone of a nucleic acid strand is made by the nucleotides form covalent bonds between the phosphate group of one nucleotide and the 3’ carbon of the sugar molecule of another nucleotide. At the end of a DNA molecule, there will be 5’ at one end and 3’ on the other end. Replication is another chemical property and that is when two complementary strands of DNA are partly separated by DNA helicases. When a replication of genetic information takes place, it is known as mitosis. Transcription was a big chemical property also. This is when RNA …show more content…

There are point mutations, insertions, deletions, and gene duplications. Mutations occur when there is a mistake during DNA replication. The results that I received in this lab all depended on the base that was given. The DNA codes that I got for part two of the lab varied, but almost every time AUG started the sequence and one of the stop codes, stopped the sequence. In part three of the lab, the results I got varied on what part of the DNA I edited and what I did to edit it. The results for part four of the lab varied by where I inserted the mutations and if I used T, A, or U. For part five of the lab, the results I got were the deletion of one nitrogen base, a protein product that consisted of a hydrophilic amino acid, and a protein with a hydrophobic head with four amino acids. The results that I got were expected based on the background information that was provided to me in the lab. I expected to see changes at mutations, insertions, and deletions. There were a few possible errors that could have occurred in this lab. The first error could have been that I didn’t label my pictures right after taking them, so they may be under the wrong labels. This would just play a role

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