On the first day of testing, a gram stain test, a KOH test, a catalase test, and an oxidase test were completed. The gram stain test indicated that the unknown species is a gram positive rod, and the bacteria showed no strings during the KOH test confirming that the species is gram positive. From these two tests, the unknown was concluded to be in the family Bacillicae. The bacteria remained yellow on the cotton swab even after the oxidase reagent was dropped onto the swab showing that the bacteria is oxidase negative. For the second day of testing, the bacteria were inoculated on an SBA plate, and the analysis of this plate was difficult. The result of the SBA plate was difficult to discern, and it was unclear if the bacteria completed beta hemolysis or alpha hemolysis. Therefore, an additional SBA test needed to be completed, and with this second test it was more obvious that the SBA plate underwent alpha hemolysis. The SBA result showing negative beta hemolysis, along with the negative result from the oxidase test, indicated that Bacillus cereus was not the identity of the unknown. At the same time that the SBA hemolysis test was undergone, the inoculation of an MSA plate at 37 degrees for 24 hours was also completed. There was yellow growth on the MSA plate indicating that it could grow at 7.5% NaCl and ferment mannitol.
An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The first procedure that was done was the gram stain. Under a microscope, if the gram stain is purple, the bacterium is gram positive, if the stain is red, it is gram negative. The next test was the fermentation tests for glucose, sucrose and
Throughout today’s society, media contributes to almost everyone’s daily life. From informative news channels to comical television shows, media proves to be effective in advertisement, releasing messages and informing the audience. Although media proves to be wildly effective in advertising, releasing messages and informing the audience, periodically destructive and misleading messages are provided to the audience and directly influencing women. Cultural critics widely agree that media tends to negatively influence women and all the critics point to research which supports the belief that women are portrayed as subordinate to men, having no
The colonies were smooth, translucent, and had a white brownish color. The Gram stain resulted in Gram positive cocci. After the Gram stain was completed, the bacteria were streaked on a Mannitol-Salt Agar plate and a Catalase test was performed. After these test were completed a Phenol Red Dextrose Fermentation tube was inoculated, and a SIM Tube inoculated.
It has recently been brought to my attention that our school is looking to adopt a sustainable menu into our meal program and I would like to give my viewpoint on the matter. As one many students here at Oxford Academy this change will affect me directly. Although people may at first oppose this conversion I think if we are able to overcome the first obstacles, this change would bring a great positive impact to the environment and the health of our students.
Into the Wild, by Jon Krakauer that tells the story of a young man who's been in his 20s, went into Alaska alone and died, however their are more about him that we did not think about. That he makes us understand the real Chris McCandless, from a bibliographic point a view. The people he met, his actions, and Chris own ideas to have one reason to go to Alaska. Chris McCandless is a humble person, he went into the wilderness to test himself the feeling of his own belief about taking a break from socially, to journey without the help of friends that to offer him help to make his journey easier. Furthermore he, does not want them and his family to be part of his odyssey journey for the sake of a test for him to survive alone without having
The two essays that I choose for the synthesis essay are Parkinson’s Alley by Joy Horowitz and The Environmental Issue from Hell: Global Warming Is the Great Moral Crisis of Our Time by Bill Mckibben. Parkinson’s Alley is about residents in California’s Central Valley town of Visalia who have been diagnosed with Parkinson’s. This part of California has the highest number of people diagnosed with the neurodegenerative disease. The pesticides that are used in the surrounding farms seeps into the water that the residents use every day, causing them to get the disease. The Environmental Issue from Hell: Global Warming Is the Great Moral Crisis of Our Time is about how global warming is a moral crisis that, if not combated
The first result of importance was the result of the Gram stain. The observations of the unknown bacteria from the slant culture after Gram staining showed that the unknown bacteria were Gram negative bacilli (Image 1). After determining the unknown bacteria was Gram negative, an oxidase test was conducted on a sample from the slant culture. The cotton swap with the sample of bacteria did not change color when the oxidase reagent was applied, thus providing a negative result. With a negative oxidase test, further tests were conducted to determine various characteristics of the unknown bacteria. A MR-VP broth was inoculated with a sample from a slant culture of unknown bacteria. After incubation, the methyl red reagent was added to the broth, and the broth turned red, providing a positive result (Image 2). An EMB agar streak plate was inoculated with a sample from a slant culture of the unknown bacteria, and after incubation, growth was found on the plate, providing a positive result (Image 3). A Citrate agar slant was inoculated, and after incubation, growth was found on the media, providing a positive result (Image 4). A Urea agar slant was inoculated, and after incubation, the agar had changed from a peach color to a bright pink color, providing a positive result (Image 5). Using the flowchart (Figure 1) developed from the Table of Expected Results, the lab partners started at the oxidase test. Given the negative result of the oxidase test, the flowchart is
The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible
This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.
Preliminary studies help identify Genus species of bacteria. Two different preliminary study pathways must be used since two different pathogens were found in the sample. A dilution and a quadrant streak are the ideal methods to separate pure cultures of bacteria. MacConkey Agar and CAN (MAC) is a selective media that is used for the cultivation of gram negative bacteria. (PEA) is a selective media that is used
At the beginning of the 20th century, American Progressive methods began changing to fit a new era of economic and social policy. The early 1900s were a time of increasing social awareness and focus on responsible legislation. Some of the economic changes that caused this new Progressive awareness and focus were desire for growth, new moral ideologies, and an orientation around workers' rights.
In Amy Chua’s essay, “The Right Road to America” she argues the point that immigration is a great value of this country and that America was built from immigrants. One thing differs from Chua’s essay than from others because she not only gives highly credible facts on how immigrants have helped our country, but she also provides ways to improve the issues of immigration into America. Amy Chua is a highly credible professor at Yale and states throughout her essay that immigrants who possess American values deserve to be here in America and those who don’t should leave. America had always been a country that accepted immigration and allowed all
The purpose of this study was to identify the unknown bacterium using biochemical tests and various methods that had been learned from previous the microbiology laboratory class. Identifying the unknown bacterium was determined by separating and differentiating possible
The bases of this experiment was to discover the identify of the unknown from three possible specimens: Klebsiella pneumonia, Escherichia coli, and Enterobacter aerogenes. Utilizaing the T streak technique, the bacteria was isolated into pure colonies for further study. The Gram Stain method was used to identity the morhphology of the bacteria such as the shape and whether the bacteria was Gram positive or Gram negative. Biochemical test were also used to help identify the unknown bacteria. The biochemical test used was the Triple Sugar Iron Agar, Sulfur Indole Motility test, Methyl Red test, Voges-Proskauer test, Citrate test, Urease test, and the Gelatin test. After observing the morphology of the bacteria using the Gram Stain method and utilizing all the possible biochemical test, the bacteria was identified to be Enterobacter aerogenes.
Education should strive to motivate students to become cultured, well rounded individuals. Unfortunately, some policies implemented by schools hold children back in this area. Homework should be abolished because it promotes unhealthy lifestyles, increases stress, and yields uncultured students. Homework stresses out children and their families. Source A depicts a depressed child sulking over a pile of homework.