Microbiology Lab Report

Add RO water to the 25 ml volumetric flask up to the mark. Put stopper on the flask and shake it properly.

Again, label 7 1.5ml tubes 0 thru 6. Place 15μl of each serially diluted extract into its corresponding labeled tube. Next add 465μl of media into each tube. Then 60μl of Alamar blue in each tube. Finally add an additional 60μl of cells (adjusted to 10,000 cells/20 μl). Vortex each tube for 5 seconds. Now, take 3 different samples 190μl samples of concentration 0 and put it in Wells A2, B2, and C2. Repeat this step again by taking 3 more different 190μl samples of concentration 1 and putting it in wells A3, B3, C3. It should be noted that it is important to vortex each 1.5μl tube again be-fore putting it into the 96 well plate. Contin-ue this same procedure consecutively for the re-maining concentrations.

After added, pick up the beaker and swirl it around lightly for a short period of time.

Purpose: To learn about the international system of units (SI), to become familiar with common lab equipment and techniques, to gain proficiency in determining volume, mass, length, and temperature of a variety of items using common laboratory measurement devices, to learn to combine units to determine density and concentration, and to use laboratory equipment to create serial dilutions and determine the density and concentration of each dilution.

11. During the 10 minutes, get the LB agar and LB+AMP agar plates ready. Mark your plates with the transformation tube mixture to use (+ or -), the lab group names, and the date on the top of the dishes.

H. How would you prepare 10 mL of a 0.25M HCl solution if 1M HCl was available? How much

Hold the inoculating loop still, move the broth culture tube up until the loop is within the

The 1 mL was then added to 9mL springwater to create the first 10 mL solution of 10 mM. The next group took 1 mL from this solution and dilute it further in 9 mL of water. This process continued for each group after. Each group took 1 mL from the previous solution until all of the concentrations were

This lab consisted of two parts over a span of three days. For part one on day one, we first began by determining the number of dilution that will be performed, and what the final dilution is going to be. My lab partner and I then disinfected our work bench to begin our procedure. To begin, using a filtered tip, we pipetted 900µL of sterile water into a labeled microcentrifuge tube for our 1:10 dilution. We then continued to keep diluting the tubes until we reached our final 1:1 dilution. We then vortexed our working stock for approximately 5 seconds and used a pipet to take 100 µL of the working stock solution to eject it into our first dilution microcentrifuge tube that contained 900 µL of sterile water. My lab partner and I used the same pipet to take out 100 µL of the first dilution to our next dilution that contained 900 µL of sterile water.

Begin by inserting a cheesecloth into a syringe and pressing the cheesecloth to the very bottom of the column.

Use the cotton swab to streak the five labeled nutrient agar plates with the E. Coli K12 bacteria from the tube.

Re-flame the neck of the bottle and place the top on. Place the disc on section 1A of the agar plate. 3. Repeat step 2 for the rest of the petri dishes (1B, 1C, and so on) 4. Place the petri dish 1 in the incubator at a temperature of 50 degrees Celsius, and leave for 24 hours exactly.

from one end and put the other end into a beaker full of iron filings,

TRIAL 2: 1) Repeat step 1 to step three from trial 1. 2) Pour 60mL in the centre of the

The LB/Amp/ara, and one of the LB/Amp plates marked with a “+” are the experimental plates.