Microglia stimulation initiates an inflammation cascade which involves NF-κB. The studies show that inflammation and the transcription factor nuclear factor kappa B (NF-κB) is a protein complex that controls transcription of DNA, cytokine production and cell survival, it is also implicated in synaptic plasticity, memory and learning. NF-κB regulates the expression of many chemokines, cytokines, like interleukins, lymphokines and tumor necrosis factor. NF-κB is involved in cellular responses to stimuli such as stress, cytokines, heavy metals, free radicals , oxidized LDL, ultraviolet radiation and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response and also has been implicated in processes of synaptic plasticity and memory …show more content…
59 NF-κB presents in an inactive state, this molecule could be either homo- or heterodimers subunit in the cytoplasm, with dimers bound by the inhibitory κB protein (IκB). In the activation pathway, NF-κB activated by many steps that are begins by the activation of IκB kinase (IKK). IKK activation then results in phosphorylation
s symptoms, supported by x-rays and biopsies of the small and large intestine. Tumor necrosis factor alpha, TNFa, a protein released when the immune system is activated, is a major catalyst in the inflammatory process and is believed to play a major role in the pathogenesis of the disease.
Protein kinases are enzymes that activate or inactivate other proteins, which is done by phosphorylation. Kinases are activated by attaching themselves to a cyclin, which is a protein that has cyclically fluctuating concentration in the cell (Cdks.) When these cyclins build up in the cell, the resulting MPF phosphorylates other proteins, overall initiating mitosis.
When injury or infection occurs inflammation ensues. Inflammation is a defense response of the body that signals for the immune system to manage/fight off infection. White blood cells and macrophages are key components of this process. On a cellular level, immunity is created by exposure to foreign antigens. When the body encounters the foreign antigens it produces antibodies to destroy the antigens. B cells, a type of white blood cell, produce these antibodies. The antigen is then stored in the memory of the B cells so that when the same antigen is encountered in the future, our body can fight off the pathogen
Tumour necrosis factor (TNF) is responsible for the disruption of the tight junction between endothelial cells which results in an increased permeability to plasma proteins and fluid, which worsens fluid accumulation in the alveoli further impairing gas exchanged (Bersten & Soni, 2009:709). TNF comprises of two different molecules, firstly TNFa which leads to programmed cell death in target cells, and when combined with IL-1 which acts on the central nervous system causing lethargy (Marieb, 2004). TNFB stimulates granulocyte activity and B cell proliferation which shows an increase in neutrophil count (Jean- Baptise, 2007:63).
Human central nervous system (CNS) is composed of neuron and glial cell ( astrocytes, oligodendrocytes, microglia) which act as structural support of brain and also contribute for formation of synapse. Microglia comprises 10% of the adult CNS. When there is tissue injury, trauma or pathogen, ramified microglia will undergo biochemical changes and turned into an activated microglia. The cell body
Nitric oxide synthase have been implicated in many aspects of human development and disease. Inhibition of iNOS or lowered levels of NO have been shown to help prevent ischaemic brain injury (4), reduce cellular markers of inflammation in osteoarthritis (5), speed differentiation and decrease survival of embryonic stem cells (6), reduction in IBS inflammation (7), protection from Alzheimer’s like symptoms in mice (8), and changes in cardiovascular function
This makes Nrf2 sense oxidative stress in the cells. Then, Nrf2 controls the tasks of the genes involved in the production and activation of vital detoxification molecules.
IL-1β induces other cytokines, such as IL-6, IL-8 and leukaemia inducing factor (LIF), causing a catabolic event by stimulating either an additive or synergistic effects (11, 24). IL-1β also cause nociceptive sensitisation through activation of intracellular kinase or the production of kinins and prostanoids (25). Hyperalgesia and hypersensitivity in joints is also associated with IL-6; primary afferent neurons responds to IL-6 and plays a role in pain transmission (1, 24). Similarly, TNF-α activates sensory neurons causing neuropathic pain via the receptors TNFR1 and TFNR2, which initiates a cascade of inflammatory responses through the production of IL cytokines (9,
NF-kB, a key transcriptional factor which have been found to be activated in a variety of inflammatory disorders such as cardiovascular, cancer and neurodegenration. Various research groups have reported the down-regulation of NF-kB by of Ganoderma in LPS induced immunological models [64,
Inflammation is the body’s response to infections and tissue injury. The inflammatory response is orchestrated by the cells of the immune system, both from the “adaptive” branch (including T- and B-cells with the capacity to induce long-term memory of encountered pathogens, “immunisation”) and the “innate” branch (including monocytes, macrophages, dendritic cells, and mast cells etc., that are targeted against common pathogen antigens). Inflammation was first implicated in AD pathology and development in the 1990’s with the neuropathological finding of activated inflammatory cells (microglia and astrocytes) and inflammatory proteins (e.g. cytokines and complement), surrounding the amyloid plaques and the neurofibrillary tangles [19]. In addition
HMGB1 is a prototypic damage-associated molecular pattern (DAMP) protein highly secreted by activated macrophages and monocytes as a cytokine mediator of inflammation. This DNA-binding nuclear protein is released both passively during cell death and actively following cytokine stimulation. It is also implicated in both infectious and sterile inflammatory disorders [32-36] affecting the central nervous system (CNS) such as in Parkinson's disease (PD) [37], multiple sclerosis (MS) [38,39], ischemic stroke [40], traumatic brain injury (TBI) [41] and Alzheimer’s disease - AD [42-44]. HMGB1 activates cells by differential engagement of several membrane receptors including advanced glycation end products (RAGE), toll-like receptor 2 (TLR2), and TLR4 which are primarily responsible for HMGB1 pro-inflammatory activity and BBB impairment [45,46]. Specific to the proposed work, several studies have clearly outlined the role of OS in the development of microvascular and cardiovascular complications of 2DM [47].
Also lowering the CSF F2-isoprostane levels in the E/C/ALA group reduced the oxidative stress in the brain. However, this raised the patient’s cognitive decline, which would is the opposite of what the next trail wanted to do.
It leads to the reduced coupling efficiency in mitochondria and increased superoxide radical generation damaging both nuclear DNA and mitochondrial DNA leading to activation of poly ADP ribose polymerase enzyme (PARP) (Fernyhough et al.Brownlee, 2001; Nishikawa et al., 2000b, to be added). PARP activation leads to inactivation of AMPK which facilitates mitochondrial biogenesis. Thus, PARP activation compromises the cellular mitochondrial genesis which leads to accumulation of damaged mitochondria in the cell. Molecular studies have revealed the involvement of transcriptional regulators such as Nrf2, NF-κB, and COX-2 inflammatory cascade in the pathology of diabetic neuropathy. The Nuclear factor 2 erythroid related factor (Nrf2) is a redox transcription factor for the production of endogenous antioxidant defenses and detoxifying enzymes. Nuclear factor-kappa light chain enhancer of β-cells(NF-κB) is a transcription factor, involved in pro inflammatory cytokine production (Negi et al.). Both Nrf2 and NF- κB regulation are co-ordinated in order to maintain redox homeostasis in healthy cells. However, during pathological conditions this regulation is disturbed offering an opportunity for therapeutic intervention. Diabetic neuropathy is a condition , in which change in expression pattern of Nrf2 and NF-κB has been reported(Koriyama et
Interleukins are a type of cytokines and play a vital role in nearly all aspects of inflammation and immunity. They were first seen to be expressed by leukocytes. Interleukin is a term that has been used to describe a group of cytokines with complex immunomodulatory functions – including maturation, cell proliferation, migration and adhesion. They also play an important role in immune cell differentiation and activation. Interleukins initiate a response by binding to like receptors located on the surface of cells; they function in an autocrine or paracrine fashion, rather than an endocrine signal, which is more common with steroidal and amino acid-derived and hormones. The response to these cytokines depends on several different factors such
Two classes of molecules (or families) can bind to CDKs and inhibit their kinase activity (Fig 4). CDK inhibitors of the Ink4 family, which consist of p16INK4a, p15 INK4b, p18 INK4c and p19 INK4d, can bind with cyclins D/CDKs only, while inhibitors of the Cip/Kip family, which consist of p21Cip1, p27Kip1, and p57kip2 proteins, can inhibit all cyclin/CDKs complexes (Fig 4) (Schafer, 1998).