Neutropenic Cancer Case Study

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The current study included 31 neutropenic cancer patients with fever, not responding to antibiotics (they were attending the outpatient clinics or were inpatient in the Hematology department of the Medical Research Institute hospital and Oncology department of Alexandria Main University hospital) during a 12 month period. Full written consents were taken from patients enrolled in the study.

As there was a fixed strategy for the management of neutropenic cancer patients involving the subsequent empirical administration of antibiotics and/or antifungal drugs, this led to difficulties in the selection of cases to reach the goal of the study. Accordingly, all patients were pyrexial, receiving antibiotics and 9 patients (29%) were receiving
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Step-one Applied Biosystems cycler was used. An aliquot of 7 μL of the PCR products was run on a 2% agarose gel. In a second step, DNA was spotted manually on LCD chips and hybridization was performed according to the instruction manual. According to the manufacturer, the array is able to discriminate between 25 different fungal species or species clusters. For scanning and final analysis a combination of a transmission light scanner and image analysis software supplied by the manufacturer software supplied by the manufacturer was used.
2.3.Method of Statistical analysis: (15) Statistical analysis was done using IBM SPSS statistics program version 21 Categorical variables were described by frequency, percentage. Chisquare test was used to study significant association between two categorical variables. Fischer exact test and Montecarlo test were used if more than 20% of excepted cell counts < 5 at 0.05 level of significance.
Kappa measure of agreement was done to detect statistical significant agreement between two techniques (e.g blood culture and microarray) at 0.05 level of significance. McNemar test was done to detect statistical significant difference in the proportion of positive and negative results between blood culture and microarray.
3. Results The study group included 19 (61.3%) females and 12 (38.7%) males. Their age ranged from one year to 60 years.

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