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Oxantrone Essay

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Mitoxantrone dihydrochloride and sodium dodecyl sulfate (SDS) were analytical grade, purchased from Sigma Aldrich and used without further purification. Experiments were performed in 0.1 M phosphate buffer pH 7.4 and 0.1 M carbonate buffer pH 10. The deionized water (18.2 MΩcm, Mili-Q water purification system) was used for the preparation of solutions.
UV–Visible absorption spectroscopy
Spectroscopic measurements were carried out using a JASCO V-630 spectrophotometer equipped with the peltier-controlled ETCR-762 model accessory (JASCO Corporation, Tokyo, JP). Absorption spectra of mitoxantrone with a varying wide concentration range of SDS have been recorded using a matched pair of quartz cuvettes of 1.0 cm optical length in the temperature range of 293.15 K to 323.15 K with an increment of 10 K interval. Mitoxantrone-SDS binding constant and micelle/water partition coefficient were determined from the monomer absorbance (660 nm) of solutions containing a fixed mitoxantrone concentration and increasing surfactant concentrations. All measurements were done at least in triplicate. Binding constant was determined by nonlinear fitting of the spectral data using the following equations [22]: …show more content…

Partition coefficient (Kx) was determined from the following equation, according to the pseudo-phase model [23, 24]:

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