Sample collection and physico-chemical analyses
Sediment samples were taken from three bioaugmented ZWE shrimp ponds having having 4500 m2 area and 1m depth located in Kerala, India: Kodungallur-Pullut farm (TCR-S; 10˚15′16.47˚N 76˚12′35.33˚E), Kodungallur-Karuppadam farm (TCR-A; 10˚16′2.68˚N 76˚11′47.18˚E) and Ernakulam-Pizhala farm, Kerala, (EKM-B; 10˚02′49.02˚N 76˚15′38.23˚E) during different phases of culturing. Based on the location and day of culture, samples were coded as TCR-S-0, TCR-S-45, TCR-S-75, TCR-S-120 for TCR-S site, TCR-A-0, TCR-A-30, TCR-A-45, TCR-A-60, TCR-A-75, TCR-A-90 for TCR-A site and EKM-B-0, EKM-B-30, EKM-B-45, EKM-B-60 for EKM-B site, where the numbers indicated days of culture. These ponds were maintained under
…show more content…
1982) using pH meter. Similarly, sediment ammonia, nitrite, nitrate was analysed. Total organic carbon (Walkley, A.; Black 1934) of dried sediment sample was determined.
Molecular analysis
Sediment DNA was extracted using the Powersoil DNA extraction kit ((MoBio Laboratories Inc., USA) following manufacturer's protocol. Recovered DNA was quantified using QuBit meter using QuBit dsDNA HS kit (Life Technologies, Portugal). Different primer pair specific to hyydrazine odi…… gene (HZO gene) was used to amplify DNA of anaerobic ammonia oxidizing bacteir (anammox). Due to low annealing temperature, the amplified product was non-specific. Later with primer (ref…) with slight modification HZO gene of anammox bacteria was amplified in a PCR reaction containing 1X EmeraldAmp® GT PCR Master Mix (Takara Bio Inc. Japan), 1mM primer each and 20-30 ng of soil DNA using the programme as follows: initial denaturation of 95°C for 5 min followed by 42 cycles of denaturation at 95°C for 60 s, 60 °C of annealing for 60 s, extension of 75°C for 2 m and a final extension of 75°C for 5 min. The pooled amplified product was ligated to pGEM-T easy vector (Promega, USA) and transformed into DH5α E. coli competent cells. The clone library was prepared from the positive transformants, and the clones were preserved using 60% glycerol at -80°C. Ninety-six positive clones were selected for plasmid isolation using alkaline lysis with resuspension
Brine shrimp have survived successfully since the Triassic period, around 200 million years this has been possible through their unique adaptations which have enabled them to avoid predators and suit their environment. They are pretty good at surviving except in space (who knew radiation isn’t good for shrimp). One of the best adaptations the brine shrimp have is their ability to survive in waters of very high salinity (up to 25%). There optimal levels of salinity are 3-3.5% but they are rarely found in waters below 6-8% salinity for the same reason this adaptation is so good, no predators occupy waters with this level of salinity. With this adaptation they are protected from much predation and have very few predators except for birds. Another reason there environment is good for their survival is that it is so widespread. Salt water lakes are found all over the world.
At the turn of the century, American readers were interested only in stories with happy endings, where goodness was praised and evil was punished. They did not particularly care if that was a false interpretation of the way life really was. When men such as Frank Norris, the author of The Octopus, wrote angrily of the injustices and poverty to be found in America, readers turned away. The Octopus made them change their minds. The course of the novel and the reality of its characters held the readers’ attention. It is so powerful a book that people had to care about the wheat growers, almost against their wishes.
The purpose of this lab was to answer the question, “Of the saline solutions of 0%, 0.5%, 1%, 1.5%, and 2% sodium chloride (NaCl), which solution will yield the highest hatching viability for the brine shrimp?” The hypothesis was that the saline solution most precise to the concentration of NaCl in seawater, which is approximately 3.5%, will yield the highest hatching viability. Therefore, the 2% NaCl solution will yield the highest hatching viability.
In the essay “Consider the Lobster”, David Foster Wallace communicates his experience in the Main Lobster Festival as a writer for a food magazine called “Gourmet”. In this essay, he explores the impact the festival had on him as he tries to question the morals of eating lobsters. Wallace initially makes it seem as the festival is a place of fun and celebration as he describes the entertainment: concerts, carnival rides, lobster-themed food, lobster-themed clothes, and lobster-themed toys (50). In spite of that, he changes his attitude as he observes that the festival is actually promoting cruelty to animals and holds a long discussion whether or not lobsters can actually feel pain. Through the use of his language and description, Wallace convinces the audience as he claims to persuade the reader to stop eating lobsters, but he doesn’t explicitly say so at any point in the essay.
Reaching a length of up to 5.5 centimeters (or about 2 inches), the bigclaw snapping shrimp is the largest member of the family Alpheidae, which are also known as the pistol shrimp. It is found in water up to 30 meters (98 feet) deep from as far north as North Carolina’s Outer Banks to the Brazilian coast. It also is able to survive in areas of rapidly different salinity; A. heterochaelis has be found in environments that range from mesohaline (having a salinity of 5 to 18 parts per thousand) to hyperhaline (having a salinity of 40+ ppt). This means that it lives in environments that range from brackish to seawater of above average salinity. It is most commonly found in areas where cover can be easily found during the day, such as an oyster reef.
Unknown lab report# 24 Introduction Microbiology is a study of organisms that surrounds us every day. It requires an educational understanding to identify organisms, and the ability to distinguish different bacteria’s. In applying the learning process of the different bacteria’s, unknown bacteria samples are given to be studied and identified.
Only one method of planktonic sampling was used so comparison with another method of sampling cannot be done. It is safe to assume that the most appropriate method of sampling in these areas was previously determined by the lab instructor. Isolation of organisms in the samples was met with some difficulty as there was very little life to be observed. Once isolated however, a general identification was simple with the use of the “quick picks” section of the Marine Invertebrate Zoology Lab Manual. One particular organism isolated from the “T-docks” samples, a foraminiferida, was chosen for scientific drawing (Figure 1).
Our end results only supported to some extent our hypothesis that increasing numbers of brine shrimp would directly cause decreases in algae concentration. Our hypothesis, if applied to only the jars of 3 and 6 brine shrimp, would be highly supported, due to the significant difference in algae concentrations. Evidently, the more shrimp that were present in the ecosystem, the less algae that were available at the end. In an ecosystem with more consumers, the consumers demand a higher amount of nutrients from the environment, or more specifically, the producers. And as the consumers develop and grow, those demands also grow. And thus, when we increased the amount of brine shrimp, more algae were consumed per unit time. Though the rate of consumption from the brine shrimp did not exceed the rate of growth of the algae in any jar, the
“They see something, walk up to it, pick it up, and maybe beat the crap out of it,” says Justin Marshall, referring to a commonly unknown creature called the Mantis Shrimp. This species often goes overlooked and unnoticed by most of the world and not just because it only grows 2-7 inches (“Peacock Mantis Shrimp” 1). Since it is such an unknown animal, many people do not know what it is or what makes it remarkable. How can the small mantis shrimp, a crustacean whose appearance is more like that of a clown than a ferocious beast, be worth noting in the different fields of science? They are more interesting than they seem. Mantis shrimp possess a vast array of fascinating physical features that set them apart from other sea-dwelling animals: their clubs
As a meat eater, has anyone ever asked the question, “How can you eat meat?” The controversy of being a meat eater or vegetarian has been around for decades. David Foster Wallace wrote a thorough article, Consider the Lobster, in which he brings up popular topics of concern over eating meat. Is it morally wrong to eat the meat of an animal, or creature, who suffered for one's own hunger? Why should one have the right to decide the standards of pain? Questions included in Wallace’s article have had many in a whirlwind of emotions and opinions. As a vegetarian, one will know the full truth concerning health, recognize false advertisements, understand how animal pain relates to human hunger, and acknowledge the treatment of animals up until their deaths.
FISH analysis showed that the new extraction method was successful for four out of seven probes used on the sludge samples. The general probe EUB 338 showed a high density of bacteria in the seeding sludge and highly reduced densities after 24 and 28 days of operation, though still some patches with high bacterial density could be detected. In the samples from days 24 and 28 cloudlike structures were observed in all wells including negative controls, which showed high light intensity. These structures coincided with reduced hybridization ability of the EUB 338 probe (cf. figure 14 G-L) Whether the background of these structures was too high, the probe directly bound to the substances, which caused these structures or the hybridizing or fluorescent ability of the
The objective of this project was to cook, and serve lobster utilizing a method, which was given, to prepare the lobster as well as a recipe for the lobster. In addition to this we also needed to create rice pilaf, asparagus, and a vinaigrette salad using the various ingredients provided to us. This project is supposed to give us our first glimpse into implementing our newly found skills from Culinary class into our cooking lifestyle at home.
The purpose of this experiment is to understand the effects of nutrient enrichment and eutrophication, using samples of water from Rio Salado and Encanto Park. The samples will contain different concentration levels of nitrogen, phosphorous and nitrogen and phosphorous combined and the impact it has on algae growth. The results recorded showed that the nitrogen concentration levels had a little change, phosphorous levels had a higher change and phosphorous and nitrogen combined had a significantly higher change, resulting in higher algae growth. The results showed that phosphorous indeed is a limiting nutrient in algae growth, but to achieve the highest growth rate, both nitrogen and phosphorous need to be combined.
Analysis of DNA from practicals 1 and 2 using the technique of agarose gel electrophoresis and analysis of transfomed E. coli from practical 2 (part B)
The chemical and reagents used for the extraction and quantitation of DNA were: Plant DNAzol (0.3ml/0.1g), 100% ethanol (100%: 0.225 ml/0.1 g, 75%: 0.3 ml/0.1 g), Chloroform (0.3 ml/0.1 g), Plant DNAzol-ethanol solution: Plant DNAzol, 100% ethanol (1:0.75 v/v), TE buffer (10 mM Tris, 1 mM EDTA pH 8.0), 1.2% agarose gel (Agarose, 1X TAE buffer), 6X loading buffer (glycerol, Tris/EDTA pH 8.0, ethidium bromide), .25X TAE buffer, Restriction enzymes and Restriction endonuclease buffers. All the chemicals used were quality grade. The restriction