Plasma Membrane Lab Report

If feeding efficiency and reproduction have a direct correlation, and a population started with equal proportions of individuals with each of three feeding types, metal spoon, metal knife, and plastic fork, the frequency of the population with metal spoons as their feeding structure will increase in the next generation. While the frequency of metal knifes and plastic forks will decrease. Furthermore, since the organisms with the metal spoon feeding structure have a higher fitness level, this population will evolve by natural selection to a point where the metal spoon phenotype will be in abundant. While the organisms with metal knifes and plastic forks phenotypes will decrease in frequency due to the lack of reproduction. Eventually, if this population persist overtime, most of the organisms, if not all, will have the metal spoon phenotype, while very few, if not any, will have the metal knife or the plastic fork phenotype.

This lab was performed to test the permeability of the cell membrane of a beet using various and extreme temperatures (high and low). The prediction of this lab is testing how much stress the cell membrane can withstand under different polarities and strengths of bonds using our understanding of the fluid-mosaic model. A spectrophotometer was used to calculate the absorbance of each variable after the beet was placed in each environment. When the lab was completed, the data stated that under each different reactant and condition the cell membrane showed multiple changes in tolerability of the beets.

The cell membranes are the utmost essential organelle that surrounds all living cells. Its purpose is to control what goes in and out of the cells and is accountable for the various other properties of the cells as well. The nucleus and other organelles also have membranes that are practically indistinguishable. Membranes are organised in a mosaic arrangement, comprised of carbohydrates, proteins and phospholipids. This can be seen in Figure 1. The objective of this indirect examination is to study the causes of various solvents and conducts on live beetroot cells. The reason why beetroot cells have been selected for this experiment is because they have a big membrane-bound central vacuole, as seen in Figure 2. The red colour anthocyanin, which provides the beetroot its bright colour is located in the vacuole. The cell membrane encloses the whole beetroot cell. The anthocyanin cannot leak out if the membranes stay unharmed. The red colour can escape if the membranes are hassled or broken.

Aim: To investigate how effect of Detergent Concentration (cont.) has on Membrane permeability of Beetroot cells. Hypothesis: I predict that as detergent concentration increases, the solution will become less clear, plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than the water potential inside the beetroot cell.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

Therefore, more of the red pigment in the beetroot would leak as the lipids control the substances that enter and leave the cell membrane.

The cell membrane (Plasma membrane) functions to provide cell support, cell stability and control entry and exit of materials from the cell. This study was conducted to test the effects of environmental conditions such as the on beet root cell membrane (Beta vulgaris). Five trials using varied pH concentrations were tested and absorbance rates were monitored. The experimental results showed that the protein function decreased sequentially when the pH decreased. This allowed the betacyanin dye to leak out which created the color that was needed to determine the intensity and therefore the effect of the circumstances. This supported the hypothesis that the more acidic or basic the environmental condition around the beet cell, the more permeable the, membrane indicated by color intensity. Pigment leakage in the solution was analyzed by using a spectrophotometer.

Beets also known as Beta Vulgaris, contain a pigment known as betacyanin that gives the beets its deep rich red color, it is water soluble and is stored in the vacuoles of the beet roots and stems. Because the betacyanin is a water soluble pigment is can not easily cross the cell membranes.

The Effect of Temperature on the Permeability of Beetroot Membrane Analysis The graph shows the colorimeter readings increase as the temperature increases, they increase by the most at higher temperatures. This is shown by a smooth curve. This means that the beetroot samples release more dye at higher temperatures.

Describe the conformation of the phospholipid bilayer of the plasma membrane. What abundant fluid leads to his conformation? Because the phospholipids heads are

This experiment seeks to analyze how the resting membrane potential of Orconectes rusticus muscle cells changes in response to increasing [K+]o solution concentrations. By recording the intracellular voltage of the DEM, DEL1, and DEL2 crayfish muscle cells at six concentrations of [K+]o solution, we determined whether the observed resting membrane potentials (Vrest) were significantly different from the predicted Nernst equilibrium potential values. We hypothesized that the Vrest of the crayfish muscles at each concentration would not significantly differ from the Nernst potential, which solely considers the permeability of potassium ions to the cell membrane. However, our findings suggested differently, and results indicated that the Nernst equation did not accurately predict the obtained values of the resting membrane potential. The differences in muscle cell Vrest reveal instead that the membrane is differentially permeable to other ions.

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

Thus it forms holes, disrupting the membrane of the beetroot core which essentially prevents the betacyanin pigment from leaving the cell. Also, when exposed under heat, the cholesterol, glycolipids and phospholipids expand, putting pressure on the membrane from inside. The lipid part of the membrane will become liquid, making it open to leakage. The proteins in the membrane will denature, increasing the permeability in the surface. The combination of vibrating molecules and a denaturing membrane would disrupt the organized structure of the membrane. And eventually with a disrupted membrane, betacyanin pigment in the innermost compartment will leak out.

Cell membrane is a selective boundary composed of a unique phospholipid bi-layer structure consisting of lipids, proteins and carbohydrates. This structure regulates the import and export to maintain homeostasis condition inside the cell. (Knox et al., 2014) The plasma membrane is referred as a fluid mosaic which also has selective permeability. The permeability of the membrane can be varied depending on the external conditions. (Mitchel, 2015)

The findings of this experiment reinforced the hypothesis that the resting membrane potential is most influenced by the ion potassium. We were able to deduce this through the collection of a multitude of intracellular and extracellular recordings, such as the one shown below in Figure 1. This shows how this experiment was able to record every single resting membrane potential in all three different muscle groups under all six solutions.