Beet Lab Report

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Beets also known as Beta Vulgaris, contain a pigment known as betacyanin that gives the beets its deep rich red color, it is water soluble and is stored in the vacuoles of the beet roots and stems. Because the betacyanin is a water soluble pigment is can not easily cross the cell membranes.

The purpose of this experiment was to alter the cell membrane of the beets, in a given fashion, so that we can test how much betacyanin was able to cross the cell membrane of the beet through various treatment. In which after we would test the absorbance level of each treatment, run it through excel and observe which treatment was most effective at getting betacyanin through the cell membrane more.

-beet (Beta vulgaris) roots
-beakers (250 ml and 1000 ml)
-hot plate
-100% EtOH
-1.0% Triton X-100 in ddH20
-spectrophotometer cuvettes
-spectrophotometer (with absorbance set at 525 nm)
-15 ml test tubes
-cork borers, scalpel or razor blade
Approach and Methodology
For this experiment we were instructed to conduct our own procedure with whichever combination of treatment we thought best would yield the greatest amount of absorbance of betacyanin. My lab partners and I chose to do treatments with ethanol a mild organic solvent, 1% triton a mild detergent solution , and a combination of ethanol and triton.
We first started this experiment by obtaining twelve 15ml test tubes, in which we placed in a rack and labeled each with what

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