Photochemical Reactions of Photosynthesis
Lillian Doubrava
Bio 111 - 568
Oct. 28, 2015
TA: Yufeng Wan
Abstract:
In this lab, we experimented with the photochemical reactions of photosynthesis. In the first experiment, by putting test tubes at different distances from a light source, we tested the effects of light intensity. In the second experiment, the effects of light wavelengths and pigments were tested on spinach disks under different colored lights over time to see which light could float the most disks. In the third experiment, by using photosynthetic pigments, we measured absorbance to see which wavelengths of light were used for photosynthesis.
Intro:
The purpose of this lab was to identify the requirements and products of the photochemical reactions of photosynthesis by specifically looking at the effects of light intensity, light wavelength, and the activity of photosynthetic pigments. Photosynthesis is amongst the most important biochemical processes on earth because it is how the biosphere gets its chemical energy, which is fixed from solar energy. Photochemical, or light, reactions are light dependent and instantaneous reactions that capture energy and split water molecules to release oxygen. They also activate the electron transport chain. In the first experiment, our hypothesis was that the medium light position, 30 cm away, would have the greatest absorption because it is not too close or too far from the light source for absorption. In the second
Light intensity is a key component in photosynthesis, amongst carbon dioxide and water to sustain a suitable rate of photosynthesis. Chlorophyll absorbs the light, causing photoexcitation and the formation of NADPH and ATP with production of O2 as a by-product. The Calvin Cycle takes the NADPH and ATP to reduce CO2 into sugars (CH2O), and return NADP+ and ADP + Pi to the light reactions. The process will then repeat. (Reece, et al, 2015)
The purpose of this experiment is to determine the maximum absorbance of fast green, and the chlorophylls, also in the case of fast green create a concentration curve to determine an unknown substance. Each test will use the spectrophotometer.
The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.
Photosynthesis occurs each time the sun’s light reaches the lives of a plant. The chemical ingrediants for photosynthesis are carbon dioxide (CO2), a gas that passes from the air into a plant via tiny pores, and water (H20), which absorbed from the soil by the plant’s roots. Inside leaf cells, tiny structures called chloroplasts use light energy to rearrange the atoms of the ingrediants to produce sugars, most importantly glucose (C6H12O6) and other organic molecules. Chlorophyll gives the plant its green color (Simon, 02/2012, pp. 92-93). Chemical reactions transfers the sun’s light energy into the chemical bonds that hold energy-carrying molecules. The most common are
photosynthesis happens in two stages: light reaction and carbon fixation also known as calvin cycle.light reaction TAKES PLACE IN THYLAKOID USE light energy to produces atp and nadph whereas, calvin cycle takes place instroma uses energy derived from light dependent reaction to make GA3P from CO2 ( Bio166 lab execise manual, 2015). the purpose of this experiment was to separate plant
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
The hypothesis that an increase in algae concentration when exposed to light would increase the rate of photosynthesis in diluted indicator solution was supported by the results of this investigation. As Table 1. Indicates the pH levels and absorbance (AU) increased in respects to an increase in algae concentration when the algae was exposed to light. However when an equal amount of algae concentration was not exposed to light the absorbance and pH levels of the algae was lower, therefore indicating that light and algae concentration increased the rate of photosynthesis in algae. Figure 1. also demonstrates that the mean absorbance of algae increased with an increase of pH levels and therefore increased the rate of photosynthesis occurring in the algae.
The Effect of Light Intensity and Temperature on the Rate of Photosynthesis Aim The aim of my experiment is to determine whether intensity of light and temperature would affect the rate of photosynthesis in a plant. To do this, I will place a piece of pondweed in varying light intensities and temperatures, and observe the amount of oxygen being given off. I am using pondweed because of its unusual quality of giving off bubbles of gas from a cut end, when placed in water. Introduction Photosynthesis occurs only in the presence of light, and takes place in the chloroplasts of green plant cells.
For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.
This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.
This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake, light consumption, and varying pigmentation.
The goal of the experiment is to determine how the type of light affects the rate of photosynthesis. If the light source is changed from a white light bulb to a LED light bulb, it is predicted that the rate of photosynthesis will increase.
In this experiment, the alternative hypothesis was supported by the results. The alternative hypothesis state that, the different colors of light will have an effect in spinach (Spinecea olerace), in the results session, it is visible that every color of the light has a measurable impact on the amount of energy that S. alerace absorbed. The colors that showed to have the biggest effect in Spinach was Red lite and white lite. The red light demonstrated to have the higher amount of energy that spinach can absorbs. The tube that showed the lowest amount of energy was the foil tube, because it was cover from any source of light. Also, this tube tested that plants that do not received light, like the foil tube, did not capture as many energy as