Should The Lysol Cleaner Be More Effective Than The Green Works? Essay

The dirtiest thing you own maybe your cell phone. Imagine what touches your phone the most, probably your hands and face. Since cell phones are everywhere and have become one of the most helpful tools of our generation, they have their drawbacks. The use of cell phones happens everywhere today, one place being the hospital. From a study of phones and spreading of bacteria associated with health care associated infections, Karabay states, “Our study reveals that mobile phones may get contaminated by bacteria (such as Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae), which cause hospital infections, and may serve as a vehicle for the spread of nosocomial pathogens” (Karabay, 2007). Touching contaminated

This experiment illustrates the importance of handwashing and proves that hand washing is worth it. Since our hands are constantly coming into contact with ourselves and others, touching surfaces, grabbing objects, being sneezed into, etc., keeping our hands clean is one of the most effective, yet simple way we can take to avoid getting sick and spreading germs to others. Many diseases and conditions are spread by not washing hands with soap and clean, running warm water. “The human skin is a host to anywhere between 10,000-10,000,000 bacteria per square centimeter and since health care providers come into contact with pathogenic bacteria by being engaged in patient care, hand washing can reduce the risk of spreading diseases (page 3).” The objective of the experiment is to test the effectiveness of hand washing and demonstrate normal flora. This report presents the procedures and materials for the experiment, the experiment's results, and an analysis of those results.

| UNIT 4222- 264 THE PRINCIPLES OF INFECTION PREVENTION AND CONTROL | | | |

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

The first step to identifying the unknown bacteria residing on the blood agar plate sent in from Khokana was to do a Gram stain on it. This is an important first step because it dictates further testing that will be necessary to arrive at a final conclusive result. Viewing the fixed and stained slide under the microscope revealed round chains of bacteria in a purple color signaling Gram-positive streptococci. A catalase test was performed with no bubbling present indicating a negative result. This further confirmed the shape and arrangement seen under microscopy. With this mind, the coagulase test was not done, as it would be of no use since that specifies for staphylococcus, specifically for Staphylococcus aureus. For streptococcus, an examination of hemolysis was necessary at this point. Shifting attention back to the original blood agar plate, gamma hemolysis was noted, thus narrowing the field down to two choices left. The unknown bacteria was either Streptococcus bovis or Entercoccus faecalis. This also means the Optochin and Bacitracin sensitivity tests would not be needed as those pinpoint alpha- and

In this experiment, an unknown bacterium was given to each individual student. The main purpose of this lab was to identify the given unknown bacteria going through a series of biochemical tests as one of the gram negative bacteria among six different Gram negative bacteria Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella typhimurium. At the very beginning, streaking method; T-streak technique was used to isolate the pure colonies. For the morphological identification of unknown bacteria, Gram Stain Method was done. Biochemical tests that were conducted for the experiment

Lab Day 1: After receiving my unknown bacteria, I streaked a TSA plate and incubated at 37°C for 48 hours. I then picked a single colony from the plate with my sterile loop and streaked a TSA slant and labeled it “Working Stock”. I did the same with another TSA slant and label the second one “Back-up Stock”. This would be the samples I used to complete the following procedures through the next four weeks to determine my unknown bacteria.

An unknown bacterium 15 was awarded and labeled at the table ready to be identified. Using the skills and test that are taught and learned in microbiology were applied into learning what the unknown bacteria culture was. There were multiple procedures and test done in order to gain all the information needed to determine which bacteria was given. In order to find what the bacteria was the first step was finding the right environment and temperature that would allow the bacteria to thrive and grow. Determining this is one of the most important steps in being able to obtain conclusive results that would allow the results of the test to be accurate and correct. Without the correct temperature and environment the bacteria will give inconclusive results which will alter and skew the end results and may lead to the wrong conclusion. By using the methods that were obtained and learned through the microbiology class allowed the skills and knowledge to determine the bacteria and execute the tests in order to determine the culture.

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

Some brainstorming was done to determine the lineup of the bacteria on two Petri dishes. Next, the experiment started with the labeling of the two Petri dishes. This was followed by a mistake that occurred, which was corrected with an orange dry-erase marker. Then our hands were sanitized with hand sanitizer, and was followed with bacteria collecting. The bacteria was collected, from the silver-ish, doorknob on the inside of the door near room B216 (with evacuation plan), and the beginning part of the handrail at the right found near the stairs of the B building opposite to Luck Hall. After that, the process was

When collecting nonsterile specimens such as urine, feces, and sputum on selective media, there are specific guidelines for not only the collection process but transportation and storage procedures as well, since the samples can begin to deteriorate at room temperature. A special swab and transport system ensures the specimen can be maintained in a stable condition for several hours. This transport system for the specimen, is a nonnutritive maintenance media which ensures the microbes can survive but not grow, a buffering system and an anaerobic environment that prevents the destruction of oxygen-sensitive bacteria. With nonsterile specimens containing a multitude of bacterial species, culturing them on selective media, will encourage the growth of only the suspected

This project involves testing various disinfectants on bacteria covered toothbrushes to see which method is most effective. The three main methods of disinfectants are hydrogen peroxide, U.V rays to kill bacteria, and antibacterial soap. Learning what is in the various disinfectants that eliminate bacteria is beneficial to understanding the experiment. Bacteria are single-celled organisms that reproduce quickly. Pathogens are bacteria that cause sickness. Once the body is infected with pathogens, those bacteria reproduce and grow inside of cells in the body. As they grow, certain bacteria can make toxins; those toxins can damage the body’s cells. Symptoms of a sickness can be caused by those toxins. Pathogens can be spread from one person to another through physical contact or contact with bodily fluids. Bacteria however can be killed by sterilization, cold temperatures, antiseptics, disinfectants, and pasteurization. The antibacterial soap mentioned is a disinfectant that reduces bacteria, which therefore reduces chance of infection. Hydrogen peroxide is an antiseptic that reduces bacteria.

Nutrient Broth and Nutrient Agar were used to inoculate bacteria taken from different surfaces. Nutrient agar plate was inoculated with a sample taken from skin surface. A sterile cotton swab was first immersed on sterile water, then, rubbed against the skin with swirling motion and transferred to an agar plate by rubbing

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

Each test performed in the lab on theses unknown bacteria have a very specific significance. With each test performed correctly the lab officer is able to move closer towards a proper identification of the unknown bacteria. After performing Gram staining and deciphering if the unknown was gram positive or negative the lab officer was then able to proceed to the next step of identification. The gram positive unknown’s reaction to the catalase test informs the tester of the Genus theyre working with. This indicates which tests to perform next. The MacConkey agar is a selective medium that only allows the growth of gram positive bacteria confirming the results received from the gram staining procedure. The NaCl growth test indicates whether or not the organism is able to