Objective: The overall purpose of this experiment is to determine how solute concentration, particle size, and a membrane’s selective permeability affect the occurrence of diffusion.
Hypothesis: In exercise one, as potassium permanganate’s molecular mass (158.03 g/mol) being less than half that of methylene blue (319.85 g/mol), KMnO4 is expected to further diffuse in the agar plate due to the negative correlation associated with particle size and rate of diffusion rate with a semipermeable membrane. With this correlation in mind, it is expected a color change would occur only within the tube, as starch would remain in a dialysis tube while iodine is able to enter the tube due to starch’s relatively larger molecular size in comparison to iodine. Additionally, a selectively permeable dialysis tube containing a higher solute concentration in comparison to its surrounding solution should gain volume and the opposite (decreased volume) with a tube in a hyperosmotic solution because osmosis of water, rather than diffusion of sucrose, from a high water concentration (distilled water solution) to that of a lower water concentration (within the sucrose bag).
Background and Introduction Within this experiment’s purpose, understanding of the cell membrane, diffusion, and osmosis must first be known. As a part of all living cells, the membrane is meant to serve as a semipermeable barrier between what is inside, intracellular, and outside of the cell, extracellular, as well as
The purpose of these experiments is to examine the driving force behind the movement of substances across a selective or semiperpeable plasma membrane. Experiment simulations examine substances that move passively through a semipermeable membrane, and those that require active transport. Those that move passively through the membrane will do so in these simulations by facilitated diffusion and filtration. The plasma membrane’s structure is composed in such a way that it can discriminate as to which substances can pass into the cell. This enables nutrients to enter the cell, while keeping unwanted substances out. Active
Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.
Dialysis tubing is a membrane made of regenerated cellulose fibers formed into a flat tube. If two solutions containing dissolved substances of different molecular weights are separated by this membrane, some substances may readily pass through the pores of the membrane, but others may be excluded.
A major determinant of diffusion in a biological system is membrane permeability. Small, uncharged molecules pass through cellular membranes easily, while most and/or charged molecules cannot pass through the membrane. The movement of water across a selectively permeable membrane, like the plasma membrane
The difference is that along with large molecules, living cells prevent molecules with positive charges and solubility. This is not representing in dialysis tubing, and is only found in living cells because the tubing is only based on molecular size (98). When referring the rate of diffusion, the concentration gradient influences the diffusion rate, based on the factors of temperature. The ability for molecules diffuse from high to low concentrations primarily depends on the concentration gradient between the two areas.(96-99). My hypothesis for the study is that in the hypotonic, hypertonic, and isotonic solutions, the direction and rate of osmosis will determine based on the concentration inside the dialysis tubing. My prediction is that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant.
Water diffuses across the membrane from the region of lower solute concentration (higher free water concentration) to that of higher solute concentration (lower free water concentration) until the solute concentrations on both sides of the membrane are equal. The diffusion of free water across a selectively permeable membrane, whether artificial or cellular, is called osmosis. The movement of water across cell membranes and the balance of water between the cell and its environment are crucial to organisms. ("Diffusion And Osmosis - Difference And Comparison | Diffen"). A semi-permeable membrane known as the cell membrane surrounds the living cells of both plants and animals. Both solute concentration and membrane permeability are
The structure of the phospholipid bilayer is a 2-layer arrangement. Basically, the phospholipid bilayer has 2 ends. One end is hydrophilic (attracted to water); therefore, the opposite end is hydrophobic and repels water. The hydrophilic ends face outwards and the hydrophobic ends face inwards. This experiment enables researchers to investigate how the cell membrane selectively chooses what cells to enter the cell through osmosis and diffusion. Within osmosis, it’s a process of what substance passes and exits the semipermeable membrane into a higher concentration to equal the outside and the inside. Unlike osmosis, diffusion is the movement of molecules transporting from a high concentration to
The following hypothesis was made in regard to effect of the concentration gradient on the rate of diffusion: The higher the concentration gradient, the faster the rate of diffusion.
Diffusion is the movement of molecules from a region of higher concentration to a region of lower concentration. The rate at which molecules diffuse can be determined by the relationship of molecular weight and that rate of diffusion through a membrane. Hypothesis of this experiment is that the fluid with higher molecular weight will diffuse at a slower rate and distance.
Exercise 1: Cell Transport Mechanisms and Permeability: Activity 3: Simulating Osmotic Pressure Lab Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Which of the following is true of osmosis? You correctly answered: c. It is a type of diffusion. 2. Which of the following occurs when a hypertonic solution is added to cells? You correctly answered: d. The cells shrink. 3. The variable that affects osmotic pressure is You correctly answered: a. the concentration of nondiffusing solutes. 4. The net movement of water would be into the cell in a You correctly answered: b. hypotonic solution.
A model cell was made of a visking tube filled with ‘cell contents’. The cell contents represent the cytoplasm of a cell structure membrane. The visking tube represents the plasma membrane of the cell. It acts as a semi-permeable barrier as
Cells are always in motion, energy of motion known as kinetic energy. This kinetic energy causes the membranes in motion to bump into each other, causing the membranes to move in another direction – a direction from a higher concentration of the solution to a lower one. Membranes moving around leads to diffusion and osmosis. Diffusion is the random movement of molecules from an area of higher concentration to an area of lower concentration, until they are equally distributed (Mader & Windelspecht, 2012, p. 50). Cells have a plasma membrane that separates the internal cell from the exterior environment. The plasma membrane is selectively permeable which allows certain solvents to pass through
All cells contain membranes that are selectively permeable, allowing certain things to pass into and leave out of the cell. The process in which molecules of a substance move from an area of high concentration to areas of low concentration is called Diffusion. Whereas Osmosis is the process in which water crosses membranes from regions of high water concentration to areas with low water concentration. While molecules in diffusion move down a concentration gradient, molecules during osmosis both move down a concentration gradient as well as across it. Both diffusion, and osmosis are types of passive transport, which do not require help.
This is an experiment to examine how the Surface Area / Volume Ratio affects the rate of diffusion and how this relates to the size and shape of living organisms.
Diffusion is the movement of particles down their gradient. Simple Diffusion is the passive movement from high to low concentration. Facilitated Diffusion is the movement of particles from high concentration to low concentration through a protein carrier. Osmosis is the diffusion of water across the cell membrane. steepness of gradient, temperature, size of molecule, and distance are all factors in the rate of diffusion. Tonicity refers to the concentration of solute in the solvent. A typical solution consists of two components known as the solvent and the solute. The solvent is used as a dissolving medium while the solute is used as the substance dissolved in the solvent. In a hypotonic solution, there is a lower concentration of solute relative to the inside of the cell. In a hypertonic solution, there is a high concentration of solute relative to the inside of the cell. In plants cells, the swelling of cells placed in a hypotonic solution results in turgor pressure. The structure of the cell stops it from bursting. Turgor pressure keeps the