Spinach Lab

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

This lab was conducted to discover which level of light (measured in lux) would make Spinacia oleracea leaf disks float the fastest. Lux (lx) is latin for light, and is the unit of measurement for light in the International System of Units (Rouse 2011). Light plays a key role in the process of photosynthesis and in this we are essentially measuring the rate at which Spinacia oleracea leaf disks convert a bicarbonate solution into oxygen. The oxygen then would bubble up, allowing

This is because the plant was in the test tube by itself, and placed in the light so the elodea could freely photosynthesize, which would eliminate a large portion of the carbon dioxide within the tube.

The materials needed for the experiment include radish seeds, sand, a scale, paper towels, a graduated cylinder, a ruler, and plastic Ziploc bags. For the 4 experiments, 20 of the radish seeds were counted per experiment, for a total of 80 seeds. Then, the mass of each group of 20 seeds was measured. Then, the 3 experimental groups were shaken in a bag of sand. Sample A was shaken 5 times, Sample B was shaken 20 times and Sample C was shaken 40 times. For the Control group, the seeds were not shaken in a bag of sand.

The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.

My lab partners and I performed an experiment that involved placing spinach disks into separate cups of distilled water (dH2O) and 0.2% sodium bicarbonate (NaHCO3) solution to examine photosynthesis in leaf tissue (Department of EEB, 2015). Discovering that the spinach disks quickly floated to the top of the 0.2% NaHCO3 solution and not in dH2O, we wondered if varied concentrations of carbonation would affect the rate of photosynthesis (PS). We tested this by halving the 0.2% NaHCO3 solution (using equal parts dH2O and 0.2% NaHCO3 solution to make 0.1% NaHCO3 solution). I hypothesize that if the spinach disks are placed in the 0.1% NaHCO3 solution, then they will have a slower PS compared to the disks placed in 0.2% NaHCO3. CO2

The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the

According to my hypothesis, the CO2 was absorb because the elodea performed photosynthesis. My hypothesis stated that “After keeping the test tubes in a light environment for 30 mins or more, the production of CO2 would decrease because more light means more photosynthesis and more oxygen.” From my experiment, the color of the carbonated water with elodea in it changed from yellow to little yellow. When the color change from yellow, it means that CO2 were absorbed and oxygen were produced as the product. This data supported my hypothesis because after 30 of light shining to the test tubes, the test tube with plant and carbonated water produce oxygen and the CO2 were absorbed.

A plant's growth ability is dependent on its ability to acquire the resources it needs to survive. Competition such as interspecific and intraspecific, limiting resources, and population density affect the fitness level of a plant. This experiment was conducted in order to test the capability of collards and radishes to grow in manipulated densities under interspecific and intraspecific competition. I hypothesized that both collard and radish plants will grow more efficiently in single species pots under low-density conditions. I also hypothesized that in the mixed species plots the radishes will be more fit to survive and grow better than the collard plants in both the high and low-density pots. Both high and low density and single and mixed species plots were planted and results were observed. There was a significant

To measure the rates of photosynthesis by measuring the change in CO2 levels in the air that is in the LeafLab chamber.

Title: Title of lab/experiment. Your name and the names of any lab partners. Your teacher's name. The date the lab was performed or the date the report was submitted. The title says what you did. It should be brief (aim for ten words or less) and describe the main point of the experiment or investigation.

The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less

For this experiment, we focused on the starch molecules that are contained within the whole-wheat seed.

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

A more detailed overlook of the experiment is demonstrated in Figure 1. The figure depicts a positive trend between the substrates and the amount of carbon dioxide produced over time. It also shows that starch produced the least amount of CO2 from the rest of the substrates and was more at a plateau compared to the other three substrates. Starch only had a 0.3 mL increase from the 1-minute