Table 1, shows the average change in mass and average rate of osmosis of the egg after it had been soaking for ten minutes in the distilled water, 1%, 2%, 5% and 10% Sodium Chloride solutions. The table also shows the SEM for each of the averages. All of the averages show that as the salt concentration increases from 0% to 2%, the rate of change in mass decreases. From the 5% salt concentration to 10%, the rate of change in mass begins to increase. This indicates that somewhere between 2% and 5%, an isotonic environment was reached. This is all shown in the results, as the average rate of osmosis in the egg after being submerged in the distilled water is 0.105 g/min, the average rate of osmosis after being submerged in the 1% solution is …show more content…
This indicates that every groups data was somewhat accurate and errors were minimal.
In figure five, the r2 value was calculated, which shows how closely the data matches the trendline given by the equation. The closer the r2value is to one, the closer the polynomial trendline matches the specific equation. In figure five, the r2value is 0.99, which means that the regression line accounts for 99% of the data. This means that one could be extremely confident that the regression line is an accurate representation of the average of the collated result. The equation of the line in figure five was also calculated, which was y=-0.0005x3+0.0083x2-0.0538x +0.1039. This polynomial equation allows for the point on the graph at which the internal solute reaches equal osmotic pressure to be calculated. Once this equation was plugged into the graphing application of a calculator and the point of inflection was found, the point of osmotic pressure was found, which is 5.53%. This indicates that the intracellular concentration of the egg is 5.53% and therefore once this percentage is reached in the extracellular environment, there is an isotonic environment. This explains as to why the egg starts losing mass around the concentration mark of 5%.
Discussion
The results that were obtained support the hypothesis that as the extracellular salt concentration
In the rubber egg lab also known as the osmosis lab, I first measured the circumference of the egg which was 6 inches. The egg had no cracks and was hard. I placed the egg in the vinegar and within seconds the egg started to bubble. These bubbles were carbon dioxide. After 72 hours the shell of the egg had started to dissolve or flake. This would be considered passive transport as the vinegar diffused across the egg shell without any force. Seventy-two hours into the experiment the membrane was exposed, and had a circumference of 8 inches. Before the egg had a shell and you couldn’t see through it but once the vinegar acted as an acetic acid it broke down the shell and left a yellow membrane that felt like rubber this is an example of diffusion. I then placed the egg in another container and put corn syrup over the egg for 24 hours. The egg had shriveled and shrunk, the water had left the egg and went into the syrup and that is what caused the egg to shrink. The corn syrup is essentially pure sugar with very little water so the osmotic pressure is very low. I then placed the shriveled egg in water and waited for another 24 hours. After observation the egg had no
The solutions are 0% sucrose, 10% sucrose, 20% sucrose, 30% sucrose, 40% sucrose and an unknown sucrose concentration. We then weighed each egg separately to the nearest gram in order to have an initial starting weight to compare to the results throughout our experiment being conducted. The eggs were then placed in each beaker for 12 intervals at a time. After every 12 minutes the eggs were taken out and weighed to see if the weight of the egg changed. With a total of five intervals (12, 24, 36, 48, 60) the steps were repeated till the egg had reached the total time of 60 minutes. The changes in weight of the eggs were then added into a data table showing the weight of the chicken eggs in grams vs. the time in minutes. In a second data table the weight changes (g) vs. time (min.) between the eggs were taken and used the difference from each time and subtracted it from the initial
Hypothesis: If the egg is placed into water, then it will increase in size since water is a hypotonic liquid because it contains more water than the egg.
In all living organisms, water and nutrients make their way to tissues and cells because of osmosis, which for all intents and purposes is essential to the survival of cells (Rao & Kaur, 2007). Besides, cells and by extension organisms also get rid of their waste products by osmosis. In animals, for instance, this process occurs in renal tubules (kidneys) that filter and clean the blood to get rid of toxic metabolites and other chemicals. In order to understand how cells acquire and get of nutrients, it is imperative to know how osmosis operates. The objective of this experiment is, therefore, to study six decalcified eggs to understand and demonstrate the process of osmosis between the inside of the egg’s membrane and multiple environments of water/solute (solutions) of different concentrations: deionized water, 20% sucrose, and 40% sucrose.
What will happen to the egg when a student tests osmosis with 3 different soultions in a cup.The student is trying to see or find the effects of osmosis on an egg.Osmosis is the movement of water molecules through a selecfively permeable membrane. Diffuision is when something spreads more widely and equilibrium means when somethings is at rest due to equal opposite forces. The student wants to find out if the egg will shrink, fill up, or change in any way.
The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution made during the study, four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.
The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.
The reasoning behind this experiment is the examine whether the rate of osmosis is changed due to a change in temperature. It was hypothesized that the rate of osmosis will increase as the temperature of the sucrose is increased. The rate of osmosis was tested by using the different jars full of different temperate water and testing how high the water rose on an osmometer over a span of 20 minutes. An osmometer is a tool used to measure rates of osmosis. The different temperatures tested on a sucrose solution were 5 degrees Celsius, 20 degrees Celsius, and 37 degrees Celsius. Rates of osmosis were higher in the hot water than in the cold water and control. The results showed that the rate of osmosis increased as the temperature increased, henceforth the hypothesis was supported. In conclusion, the experiment showed how changes in temperature affect the rate of osmosis.
4. Take a piece of dialysis(as cut earlier), find the mass of the dialysis, and the zero out the scale on
An egg has a semi-permeable membrane, thus processes like osmosis could occur. Osmosis is the movement of water molecules from an area of higher water concentration to an area of a lower water concentration. Osmosis is important, especially for living organisms, as they help distribute nutrients in the body. An egg’s mass would change when it is soaked in different substances. The goal of this experiment was to investigate how an egg changes through osmosis. This experiment was done to show how substances affect the mass of an egg. To start off, an egg’s mass was measured by putting the egg in a beaker then placing it on a mass scale. The beaker was then filled with vinegar and left alone for twenty-four hours. After a day, the egg was taken
Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is
This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.
In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which
Four eggs that were previously soaked in vinegar were placed into four beakers with different levels of a glucose solution. The four beakers were filled with distilled water, 0.5M glucose, 1.5M glucose and 2.0M glucose. After the eggs were placed in the solutions, they were left for 60 minutes but weighed every 15 minutes to record whether there was an increase or a decrease in mass. The various masses were recorded as well as the percent change in mass. These were then recorded as line graphs. From this it was able to be determined if the egg had been in a hypotonic, hypertonic or isotonic solution, thus being able to determine if osmosis had occurred.
Group 1 received 107% for 0.0m, 109% for 0.25m, 112% for 0.5m, and 126% for 0.75m. Group 2 received 100% for 0.0m, 107% for 0.25m, 116% for 0.5m, and 117% for 0.75m. Group 3 received 100% for 0.0m, 106% for 0.25m, 114% for 0.5 m, and 117% for 0.75m. Group 4 received 100% for 0.0m, 106% for 0.25m, 113% for 0.5m, and 116% for 0.75m. Group 5 received 98% for 0.0m, 105% for 0.25m, 110% for 0.5m, and 115% for 0.75m. Group 6 received 104% for 0.0m, 108% for 0.25m, 111% for 0.5m, and 104% for 0.75m. The trend line that occurred for the rate of osmosis was an upward increase as the solute concentration increased.