Curcuma are highly important genus of Zingiberaceae mostly contain spice plants with very high medicinal value. Among them few species are being cultivated but majority of them are wild, endemic or endangered in nature. Therefore, these species are being depleted from nature due to extensive collection, biopiracy and habitat destruction. Curcuma longa have very well explore species but other two non-conventional species of Curcuma like Curcuma caesia and Curcuma aromatica species are still unknown due to their unavailability and extensive use by the tribal people for medicine as well as spice. Traditional methods based on phenotypic observations for identifying Curcuma varieties in Curcuma spp. are slow and have limitations. However, new …show more content…
The knowledge of genetic variability is a pre requisite to study the evolutionary history of a species, as well as for other intraspecific variation, genetic resource conservation etc. (Islam et al. 2007). Hence, genetic diversity and gene differentiation through molecular marker analysis are essential for their taxonomic relationship evaluation, conservation and sustainable utilization. For proper conservation programme it is essential to characterize the plants genetically. Number of molecular markers is being regularly used for studying genetic relations, population genetics, genetic characterizations in different plant groups and cultivars. The molecular markers are not influenced by the external environmental factor unlike that of morphological markers and hence accurately testify the genetic relationship between and among plant groups. Molecular markers like RAPD, ISSR and SSR are being used regularly for genetic diversity assessment as a thorough knowledge of the level and distribution of genetic variation is essential for conservation (Dreisigacker et al. 2005; Sharma et al. 2008; Naik et al. 2010; Das et al. 2011). PCR-based DNA fingerprinting techniques like RAPD, ISSR and SSR are proven to be very informative and cost-effective
One of the most important Brassica rapa features is that many generations can be grown in a short period of time for experimental analysis and comparison(Tsunoda, 1980).Brassica rapaplants are involved in many research works recently in which they are crossed with other crops to modify their genetic fitness(Tompkins, 1990). The Chi-Square ( ) test was used in this experiment to determine whether the statistical data supports or rejects the hypothesis.
The general approach of this study will follow similar methodology to the approach used in Ward 2006. The Genetic diversity within the populations will be examined based on samples locus genotypes and the genome diversity can be examined for fits to Hardy-Weinberg expectations. The genetic differentiation between the samples will be quantified using the FST
To decipher if a species by its morphology can be suggested as a hypothesis, but the results of its DNA will identify the species accurately. Tissues samples can be taken from the species in question, and the DNA can be extracted from tissue. Once the DNA is extracted it can be amplified. DNA can be amplified by the PCR procedure, in which specific gene regions can be used as barcodes to identify the species. These specific regions are known as Cytochrome oxidase 1 and Cytochrome B.
Grain amaranths (Amaranthus spp.) consist three cultivated species namely, A. caudatus L., A. cruentus L., and A. hypochondriacus L. In ancient times, grain amaranths were basic and staple crops in the Americas, where these originated before the arrival of the Spanish Conquistadors (Sauer 1950). From the archaeological records, the history of their cultivation has been considered to be about 6,000 years (Sauer 1969). Thus, it is expected that these three grain species had accumulated genetic variation in their population throughout this long period. To evaluate genetic variation is important for genetic improvement of the crop. Local and exotic germplasm can be used as a source of genetic variation. At the same time, a large scale of analysis
DNA barcoding uses standard genetic markers to compare DNA sequences among existing species by scanning for polymorphisms in standard sequences to differentiate between species (Hartvig, 2015). It is effective in differentiating between phenotypically similar species and is applicable to all organisms of life (Dudu, 2016). For DNA barcoding, the DNA is isolated from a sample and standard genetic markers are amplified by Polymerase Chain Reaction (PCR). A polymorphism is differences in DNA sequence that accumulate over time (Albert, 2011). The main source of mutations occurs during DNA replication and, thus mutations can be inherited. When the frequency of the mutation increases, it can become fixed in a lineage (Albert, 2011). Polymorphisms can indicate common ancestry among individuals by comparing standardized sequences across a species (Stoneking, 2001). Specifically, one region in the
Shakespeare uses stylistic texture to establish self obsession as the greatest obstacle to love, and to justify Olivia’s character change later in the scene.
Turmeric or curcuma longa originates from some tropical areas of Asia. It will grow to about three feet and its leaves are giant and green, and its flowers are pink. This plant adds interest and texture to garden beds. Turmeric are often grownup as a container plant also. Turmeric is sometimes grown for its flavourous, bright orange roots. Turmeric’s medicative properties are loved throughout history and today it's used as an anti-inflammatory drug and other people use it for treating numerous skin conditions and additionally to improve the gastrointestinal health.
This genetic material is known as deoxyribonucleic acid, or DNA. Different genes of those variations are known as alleles (Upadhyaya, 2017). Alternating forms usually result in the traits, either recessive, and dormant or dominant and visible. The primary technique used in this experiment is that of DNA fingerprinting and it uses the information stored in the alleles and genes to identify the individuals. DNA fingerprinting is the characterization of an individual’s genomes.
In Table 1, the data presents the values for calculating the strawberry DNA yield and the DNA yield value. The value of the concentration of the Methylene blue-stained DNA solution is obtained from the standard curve in Figure 1. The value was also obtained using a strawberry DNA extraction process. In this process, a DNA Extraction Buffer containing detergent and salt (sodium chloride) was used. The detergent helped in breaking down the strawberry cells to release the DNA into the solution. Water wasn’t used in this process because the phospholipids in the cell membranes wouldn’t be dissolved. The salt in this process helped in creating an environment for the DNA strands to gather and clump together. This way, it would be easier to observe
Genetic diversity is the variation of heritable characteristics in genetic makeup present in a population of the same species i.e. variation in the nucleotides, genes, chromosomes, or whole genomes of organisms. The species with greater genetic diversity has a chance of long time survival. Knowledge of diversity in a germplasm is very important for the improvement of crop plants through breeding program (Hallauer and Miranda Filho 1988). Assessment of genetic diversity is essential to understand the relationship among and within the landraces, as well as their genetic structure. It is also valuable for setting priorities for genetic conservation, parental selection and as a source of favorable traits.
One of the most common DNA fingerprinting procedure is Restriction Fragment Length Polymorphism. This procedure focuses on repetitive
Four years have passed since the discovery of DNA fingerprinting. During those four years people have learned that the four probes known to allow DNA fingerprinting in the human (M13, Jeffreys’ core sequence, the human α globin hypervariable region [HVR]) were checked for their ability to reveal “genetic barcodes” in animals(Georges et al.). Jeffreys’ core sequence, and the Per probe uses the four different probes(Lippincott). Depending on the particular probe-species combination, the fingerprints are polymorphic enough to be used efficiently in animal identification, paternity testing, and as a source of genetic markers for linkage analysis(Butler). These markers should substantially accelerate the mapping of genes affecting economically important traits(Roewer). If this revealed “genetic barcodes” in animals then the same could also be done for humans and it could possibly help out with the Human Genome Project that was started in 1990(Friedland). James D. Watson constructed the Human Genome Project was for further biological study to discover all the estimated 20,000-25,000 human genes to make it accessible(Friedland).
Results showed that all six consensus sequences were 100 % identical All six MinION sequences from both independent MinION run alignment using this method contained near identical phylogenetic tree topologies (Table 7, Figure 3) and assigned the expected genotype with up to 99.86 % identity.
are real the SNPs governing canopy height. The result could rather be false positive. Even though there is variation in population structure (figure 8) GWAS analysis failed to reveal any SNPs with high Linkage Disequilibrium (LD) for canopy height derived from image data. However, this finding is useful as it indicates that we might need some covariant like environment and/or more population structure extraction for future work. predict the height in a particular range for all the lentil varieties.
Fingerprint analysis by HPLC is widely used for the quality control of herbal drugs and displays high specificity for their identification [10-17]. To increase control over the quality of Ribonucleic Acid for Injection II, we studied and established the fingerprints of the injections in HPLC chromatograms.