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The Metabolic Rate Of Goldfish

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Introduction
To determine the metabolic rate of a goldfish two different methods can be applied, direct or indirect calorimetry. Direct calorimetry analyzes the exothermic reaction when ATP is produced by measuring the amount of heat that is released. Meanwhile, indirect calorimetry measures the amount of carbon dioxide or oxygen because both are components of aerobic respiration, a process which repeatedly supplies more ATP to match the demands of metabolic rate of an organism. Evidently, metabolic rate is the cumulative sum of energy used by all the cells. Most of this energy comes from regulating homeostasis, locomotion and thermoregulation. On the other hand, ectotherms like goldfish have a slight difference in their metabolic rates because their internal temperature directly correlates with the temperature of their environment. For this reason, ectotherms use less energy because they do not need to worry about thermoregulation, maintaining constant body temperature. However, temperature, size, amount of light and stimulus are factors that can affect metabolism of goldfish. Thus, this experiment will measure the metabolic rate of goldfish through in direct calorimetry. Furthermore, the amount of oxygen in the chamber reveals the amount of cellular respiration of the organism. While also, test the effects of decreasing oxygen, and later increasing the heat on the metabolic rate of goldfish. I hypothesize that an increase in temperature will increase their metabolic rate

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