Thin Layer Chromatography Lab Report

Adsorption chromatography occurs when “one substance form[s] some sort of bonds to the surface of another one”, creating intermolecular forces between the two substances (Chemguide). For thin layer chromatography, the components of the mixture are adsorbed onto the stationary phase that covers the plate (Chemguide). The more polar a substance is, the more strongly adsorbed it is (Chemguide) and the strong intermolecular forces then result in a slower rate of migration with respect to the moving phase. As the solvent touches the TLC plate, the solute (mixture) is allowed to move up the TLC plate

The three compounds that were separated was anisole, benzoic acid, and o-toluidine. Benzoic acid is more polar than anisole and o-toluidine which causes it to travel the TLC plate the least because the silica gel is polar. Anisole is nonpolar which causes it to travel up the TLC plate. The amount the compounds travel can help determine Rf values in order to find which eluent is suitable. 70% hexane and 30% ethyl acetate showed that it was a suitable eluent because the three mixtures match up their compounds on the TLC plate and Rf values.

This purpose of this lab is to use gas chromatography to identify unknown compounds. In this lab, six known substances were first tested for their retention times to be used as standards when figuring out the three substances contained in a known mixture. The vernier mini gc in this experiment, just like all the other types of chromatography, have both a stationary and mobile phase. With this instrument, the stationary phase is a metal outer column and the mobile phase is the atmospheric air, which is how the vernier mini gc works. This instrument is used alongside a computer to be able to obtain the readings of the injected substances and shown as peaks on a chromatograph. The specific time it takes for a substance to exit the chromatography

Me and my lab partner, obtained a mixture of a un known proportion from the instructor and then flow the guide line in our lab manual to separate the mixture by applying the separation method motioned in our lab manual pages 33-40 . In this experiment, the separation methods were decantation,

Aim The objective of this experiment was to separate a mixture of two compounds (ferrocene and acetylferrocene) using adsorption column chromatography. Introduction Adsorption column chromatography is a technique that uses a solid stationary phase which is fixed and a liquid mobile phase that moves slowly through the packed column. This technique uses the property of polarity as the compounds have a greater affinity towards the respective phases (either stationary or mobile) which leads the compounds moving at different rates through the packed column and separating from one another.

Brianda Mendez Lab 05 Report Introduction: Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction. Thin-layer chromatography or TLC, is a solid-liquid form of chromatography, it involves the distribution between two phases. The stationary phase is a polar adsorbent, it is coated on a glass slide or plastic sheet creating a thin layer of the particular stationary phase. The mobile phase, a liquid or a gas flows through the stationary phase and carries the components of the mixture with it.

Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities.

The purpose of this lab is to find out how components of a mixture can be separated and analyzed.

Gas-liquid chromatography (GLC) is a process where an unknown organic sample is dissolved in a solvent then vaporised to separate it into its’ components. This is carried out by using two phases; the stationary phase and the mobile phase. The mobile phase is the gas containing the sample and the stationary phase is a liquid absorbed in a solid support. The liquid can be changed depending on the mixture being tested, so the stationary phase is packed in to a long, thin tube called the column (4college, 2016). Gas-liquid chromatography has many uses in forensic chemistry such as determining the identity of a fuel used in a deliberately lit fire.

The chromatography process works by differences in polarity of the compounds present. Organic molecules will bind to fine particles of the silica gel by intermolecular forces. Non polar compounds such as aromatic compounds bind to the silica gel via weak van der Waals forces. Polar compounds will bind to the silica gel more strongly via dipole-dipole interactions, hydrogen bonding. When examining TLC plates the following general rules will apply: The role of the solvent is to move the compound through the solid media. A non-polar solvent will dissolve non-polar compounds. The non-polar compound will move most rapidly through the solid media with the solvent. A polar compound is more tightly bound by the silica gel via stronger intermolecular

The next stop on the coiled column is the detector, which identifies the amount of each compound is present in our sample. There are a series of factors that affect the rate at which compounds reach the detector which will be described in the following sections, but for now it is important to note that the lower boiling point and the less molecular weight, the faster the sample components will separate and migrate to the detector (McNair & Miller, 1998). Any individual peak produced can widen or enlarge as the chromatographic analysis ensues (McNair & Miller, 1998). In addition, the area of each peak is

After this, assuming no complications, the ratio of these compounds is 2.0 to up to 4 or more at 40 weeks. Infants who have a ratio of 2.0 or more rarely develop RDS (Wardelab.com, 2017). It denotes alveolar stability and lung maturity. After extraction, the L:S ratio can be measured using Thin Layer Chromatography (TLC). TLC is a useful technique for separation and identification of a compound(s) when in a mixture. Compounds are separated by their differences in their solubility. There are two phases to separate the mixtures, mobile phase and stationary phase. In this experiment the mobile phase is the solvent which is chloroform:methanol:water in ratio 13:5:0.8 v/v. The stationary phase is the TLC plate, coated with silica gel. When the samples are spotted at the bottom of the TLC plate. It is then placed in a container containing the solvent (mobile phase). The solvent will then move up the TLC plate by capillary action, moving the mixtures at different rates, thereby separating them. When the solvent nearly reaches the top, the TLC plate is removed and the solvent front is

Chromatography is a technique used to separate mixtures of substances into their components^2. It can be used to various activities such as separating different pigment of dyes and inks all the way to analyzing DNA sequences.

In thin-layer chromatography a liquid is pumped across a bed of particles. The liquid that is pumped across is called the mobile phase and the particles are the stationary phase. A mixture of the molecules that will be separated is put into the mobile phase. Thin-layer chromatography tells you/helps you determine the number of compounds in a mixture, the purity of a compound, and the identity of compounds if you have examples to pull information from. Thin-layer chromatography is used to separate nonvolatile mixtures. The dye that was the most polar was the color red and pink which was Rhodamine B and the least polar was the light pink color which was Sudan IV. Our first TLC plate had five

The first part of the experiment, 3.001g of a biphenyl/p-toluidine sample was obtained. The solid sample was dissolved in 10ml of dichloromethane. A TLC was constructed with a 20% EtOAc/Hexane as the solvent system; after it was completed, Rf values and observation were recorded. The solution was then added to the separatory funnel, making sure that the stopcock of it is closed before the solution is added. After that, 10ml of 3M HCL solution was added into the separatory funnel. The stopper of the separatory funnel was placed. To mix the layers together the separatory funnel was held in the following way: