Micro-extraction of urine and/or stomach contents and analysis by TLC

1351 WordsNov 28, 20136 Pages
Micro-extraction of urine and/or stomach contents and analysis by TLC Abstract Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities. The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs, of which there was five, using a specific TLC method. The same technique was used for the determination of the unknown; however, the solution used for each method was slightly different. Furthermore, the mobile phase in each method was of different concentration with the addition of ethylacetate in Solvent system A. The distance moved by the spot…show more content…
Unknown Y Development time – 15 minutes Distance migrated my solvent front - 57mm Sample Distance moved by spot (mm) RF= Distance moved by spot Distance migrated by solvent 1 50 0.8 2 52 0.9 3 36 0.6 4 49 0.8 5 15 0.2 Unknown Y 35 0.6 Table 1.2 – A table showing the distance moved by the spots for each particular sample and there associated Rf values for unknown Y. The solvent front was calculated by measuring the difference between the initial line that was marked 2cm from the bottom of the plate and where the solvent front finished. Both table 1.1 and 1.2 show how far the solvent front migrated up the TLC plate and how far the spots for each sample moved. From this information, the Rf value was calculated by dividing the distance moved by the spot by the distance migrated by the solvent. For example, for unknown X, the distance moved by the spot was 36mm and the solvent front migrated by a total of 61mm across the TLC plate. Thus Rf value is, 36/61 = 0.59. The same method of calculation was used to work out the remaining Rf values. The results show that for unknown X in Solvent System A, the spot travelled a distance of 36mm. According to the distance moved by each spot the closet match was either sample 1 (paracetamol), which travelled 38mm or sample 3 (quinine), which travelled 37mm. Since unknown X could not

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