Introduction
There are two types of nuclear division, mitosis and meiosis. Mitosis is usually used for the growth and replacement of somatic cells, while meiosis produces the gametes or spores used in an organism’s reproduction.
Mitosis is the first of these studied in this lab. It is easily observed in cells that are growing at a rapid pace such as whitefish blastula or onion root tips, which are used in this lab. The root tips contain an area called the apical meristem that has the highest percentage of cells undergoing mitosis. The whitefish blastula is formed directly after the egg is fertilized. This is a period of rapid growth and numerous cellular divisions where mitosis can be observed.
Just before mitosis the cell is in interphase. In this part of the cell cycle the cell will have a distinct nucleus and nucleoli where the thin threads of chromatin are duplicated. After duplication the cell is ready to begin mitosis and its starts with a step called prophase. In prophase, the chromatin thicken into distinct chromosomes and the nuclear envelope breaks open releasing them into the cytoplasm. The first signs of the spindle begin to appear. Next the cell begins metaphase, where the spindle attaches to the centromere of each chromosome and moves them to the same level in the middle of the cell. This level position is called the metaphase plate. Anaphase begins when the chromatids are separated and pulled to opposite poles. Then, the final stage is telophase. The nuclear
More than one celled organisms grow by way of mitosis and the cytoplasmic division of body cells. On the other hand, meiosis occurs only in germ cells, which are put aside for the formation of gametes (sperm and egg). Reproduction by meiosis allows for species survival and it increases genetic variability.
Exercise 3A is a study of mitosis. You will simulate the stages of mitosis by using chromosome models.You will use prepared slides of onion root tips to study plant mitosis and to calculate the relative duration of the phases of mitosis in the meristem of root tissue. Prepared slides of the whitefish blastula will be used to study mitosis in animal cells and to compare animal mitosis and plant mitosis.
In this experiment first the stages of an onion cell undergoing mitosis are going to be observed and every stage is going to be detected and drawn on paper. A brief description to what is going on should be attached to the pictures. This is important to understand the basics of cell division which is necessary growth,repair and asexual reproduction. Second the number of cells undergoing each phase is going to be counted to figure out in which phase the cell remains the most. If interphase is the stage in which the cell grows and prepares for cell division then the
According to the data table, prophase is the longest stage of mitosis. Even though there were more tallies in the interphase section, interphase is not considered a stage of the mitotic cycle; it is more or less considered a stage in the cell cycle. Telophase is the shortest stage because there were very few cells that showed evidence of them actually being in this stage. Metaphase and anaphase are intermediate stages, with metaphase being longer than anaphase.
These bivalents line up along the equator during metaphase I, the arrangement of the bivalent is completely random and relative to the orientation of the other bivalents, this is known as the independent assortment of chromosomes. This is followed by anaphase I where the homologous chromosomes separate and move to the opposite poles of the cell. At telophase I the cell divides into two, each cell contains one chromosome from each homologous pair. The second stage of meiosis is similar to mitosis.
Mitosis and meiosis are similar in several ways and different in others. The similarities include that both processes involve IPMAT. IPMAT is interphase, prophase, metaphase, anaphase and telophase. The parent cells are diploid. They both end with cytokinesis. In Metaphase and Metaphase II, the sister chromatids line up along the center. Then in Anaphase and Anaphase II, these chromatids are split and pulled towards the centrosomes. The differences are that mitosis consists of 1 division while meiosis consists of 2. Four genetically different, haploid sex cells are the products of meiosis and 2 identical, diploid somatic cells from mitosis. Mitosis occurs in all organisms except viruses and meiosis only occurs in plants, animals, and fungi.
A) Meiosis consists of two cell divisions and is broken up into Meiosis I and Meiosis II. At the beginning of the Cell Cycle, in this case there are four chromatids each from the homologous pairs being A, a, B, b. This is the Diploid number (4) meaning it is 2 times the haploid number that will be seen at the end of meiosis II. During the S phase of interphase, the chromatids replicate and reach the end of G2 phase. Now starting meiosis, during the first stage of prophase I the chromosomes condense and pair up through synapsis with their sister chromatids creating AA, aa, BB, bb. After they pair up they go through a process called crossing over, where the homologous chromosomes share a piece of their genetic material with each other. Crossing over allows for the genetic diversity of chromosomes. Now there are four homologous chromosomes Aa, Aa, Bb, Bb, each containing heterozygous alleles because the sister chromatids exchanged genetic information with their homologous pair. During late prophase I, spindle fibers being to form where they will later attach to a homologous chromosomes centromere. The next stage is Metaphase I. During metaphase I, the homologous pairs line up at the metaphase plate, also known to be the center of the cell. The homologous pairs form a tetrad which is considered a group of four homologous chromosomes. These homologous chromosomes orient themselves randomly, which is know as the process of independent
Although mitosis is just a fraction of time compared to interphase it can be divided into four distinct subphases. If we start viewing a cell under a brightfield microscope right at the end of interphase subphase G2 we would see that throughout the interphase process (fig 1) the cell has grown considerably in size due to production of extra mitochondria and endoplasmic reticulum. The cell has also gone through the labor of duplicating all of its chromosomes, a process known as DNA synthesis. Now that the cell has made all of these preparations it's time to start prophase (fig 2), the first stage of the mitotic phase. This stage is visibly identified by the chromatin becoming the tightly formed chromosomes. During the lab we were able to view these newly formed chromosomes best on the onion root tip
Find a representative cell for each stage of mitosis on the onion slide and draw a clear diagram of the different phases you observed in the accompanying space. Be sure to draw only what you see, but include all details that are visible. Your drawings will not necessarily look exactly like the ones in Figure 3A, 3B, 3C.
Mitosis is the process of duplicating nuclear material one cell becomes two cells. A cell contains 46 chromosomes, this is known as diploid. This process involves prophase, metaphase, anaphase, and telophase. Mitosis ensures the continuation of the organism and helps the body build and repair. Meiosis on the other hand is for production of haploid cells, 23 chromosomes, that together with another human, new organisms are created.
The phase in mitosis which chromatin begins to coil and the mitotic spindle begins to form.
19) The phase of mitosis during which the nuclear envelope fragments and the nucleoli disappear is called
cell will look just like any other 'normal' cell although this is far from the
Mitosis is one of two main methods of cell replication, the other being meiosis. It is “the simple duplication of a cell and all of its parts” resulting from the splitting of a cell. The ‘parent’ or original cell splits, duplicating its DNA (packaged in chromosomes) producing two ‘daughter’ or new cells with the same genetic code. There are four stages of mitosis; Prophase, Metaphase, Anaphase and Telophase. Interphase is not an official phase of mitosis as the cell is at ‘rest’ and not being divided during this phase. It is often called one of the phases of mitosis as it is one of the stages in the life cycle of a cell; however it is not a phase of mitosis due to the fact that no division takes place.
There are four stages of mitosis. The first stage is the prophase. In this stage the chromosomes become