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What Is Cambrian Explosion?

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Cambrian explosion is one of the great evolutionary events of animals. Before this event (635 to 541 Mya), most animals called Ediacaran fauna were almost flat and soft bodies (ref). However, in early Cambrian (around 550 Mya), most of modern phyla of animals have evolved with skeletal tissues were remain as fossils (ref). This direct evidence suggested that the mechanisms of skeletogenesis have evolved in the near base of Bilateria in the earliest Cambrian period, and the Cambrian explosion might have been due, partly, to the acquisition of this novel body plan. The common ancestors of Ecdysozoa or Lophotrochozoa have evolved the external hard tissues, exoskeleton, and became diversified morphological and mineral structures in each phylum …show more content…

Mollusca are second only to arthlopoda in the number of animal phyla and have evolved one or more exoskeletons called “shell”. Their initial shells are secreted in the shell gland that is formed by the invagination of ectodermal cells (ref), and the gene engrailed expressed the boundary region of shell formation and corresponds with their different number of shells per body (Fig. 1a; Polyplacophora, Jacobs et al. 2000; Cephalopoda, Bratte et al. 2007; Scaphopoda, Wanninger and Haszuprunar 2001; Bivalvia, Jacobs et al. 2000, Gastropoda, Moshel et al. 1998). In order to investigate engrailed function in molluscs, we focused on retinoic acid (RA; a vitamin A-derived morphogen) pathway. Because RA pathway has been well known as one of the signal pathways that regulate transcription factor genes in vertebrates (e.g. Hox genes, Marshall et al. 1994; engrailed, Holder and Hill 1991). RA is mainly synthesized from retinal by the retinaldehyde dehydrogenase, Aldh1a, and degraded to oxidized retinoic acid by the cytochrome P450 RA-hydroxylase, Cyp26 (Fig. 1b). Thus these two enzymes regulate the saptio-temporal distribution of RA levels during embryogenesis (Niederreither et al. 2002; Reijntjes et al. 2005). In molluscs, RA signaling machinery have already found from the genome database in gastropods (limpet; Lottia gigantia; ref) and bivalves (pearl oyster; Pinctada fucata; ref). However, it has been unknown whether RA pathway regulates engrailed expression like zebrafish and correlates with shell

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