Essay on miss

With these absorbance numbers a concentration curve was constructed and the unknown solution was determined by finding the point of absorbance on the curve.

The protein molecules in many foods provide the amino acid building blocks required by our own cells to produce new proteins. To determine whether a sample contains protein, a reagent called Biuret solution is used. Biuret solution contains copper ions. However, the chemical state of the copper ions in Biuret solution causes them to form a chemical complex with the peptide bonds between amino acids (when present), changing the color of the solution. Biuret solution is normally blue, but changes to pink when short peptides are present and to violet when long polypeptides are present.

The Beers Law calibration experiment used many concentrations of crystal violet solutions. Each of these solutions were test and analyzed in order to determine the absorbance of each concentration The results were than graphed and produced a slope of 1.00E05 with an intercept of -2.21E-02.

5. The degree of precision was to 3 significant figures obtained with the spectrophotometer. The major source of error in our experiment was not calibrating the spectrophotometer with distilled water.

The absorbance with SDS shows a rapid increase, before the graph begins to increase more slowly and uniformly. The absorbance levels off at 0.9nm. The absorbance with no SDS remains at a constant level of 0.01nm for the entire experiment. The absorbance of the control solution is also constant throughout the experiment. This shows the absorbance of the compounds in the solution without the ovalbumin, by taking this figure away from the other recordings, it is possible to discover the absorbance for ovalbumin alone.

To identify systematic error the experiment must be repeated. When repeating the experiment new apparatus or materials must be used, such as new stock solutions of hydrogen peroxide. If the new data differs consistently from the original data this indicates systematic error is present.

10 microliters of the sample is then added and the assay absorption is measured at 340nm. If absorbance was above 1.5, samples were diluted.

Incorporation of assay controls included setting up a spectrophotomer and running the chart recorder with a full-scale deflection before the start of the assay. The set recorder had a corresponding value of 1 for the change in the absorbance. Therefore, prior testing was done to observe whether a change occurred in the readings. This helped to indicate that the results were valid, as they could have been affected by a fault during the setting up of the spectrophotometer. On the other hand this was considered as one of the controls for the experiment. Nevertheless, a new cuvette had to be used for each assay.

According to the scientific method, I should create a new experiment by making another hypothesis.

The absorbance is measured using a Plate reader and a Standard curve is generated. Also, the different types of pipetting techniques are assessed in this Assay.

Amylase concentration, the equation is y= 0.0382x - 1.0162. The R2 value is 0.92732. Serial dilutions were made using individual saliva, with measurements of 10x, 100x, and 1000x. From these dilution factors, the closest number to the absorbance curve was 1.4829ug/mL, which is my amylase concentration in saliva. During lab 6, the amylase saliva DNA was put into a PCR chain reaction and then in lab 7 it was put into a gel electrophoresis.

For testing for proteins, take the well plate. Put about 2-3 mL in of the substance being tested for proteins in one of the holes using either a pipet or a small spoon (depending on the substance). Drop about 2 mL of Biuret reagent in the well plate hole using a pipet. Thoroughly stir the Biuret reagent in with the substance using a toothpick. Continue these steps for testing the next substances for containing proteins.

The purpose of the experiment was to see if the object was alive. The hypothesis was if the object was alive it would grow in water. After adding water the data recorded indicated that the object had signs of life. The object turned from brown to a green the object grew and became softer. A blunder error could have been made in the process. The object could have been flooded with water because of an incorrect measurement. The hypothesis was supported by the data indicated that the object was alive.

The same solution of 0.5 ml BSA was then added from test tube 1 to the test tube 2 after being properly mixed, and from test tube 2 the solution was being added to test tube 3, and so forth all the way up to test tube 5, with the same exact procedure. From the last tube, we then disposed the 0.5 ml solution. After above procedures, we now labeled another test tube “blank”; 0.5 ml blank distilled water was purred into the tube with the serial dilution of 1:10. We also had a tube C labeled “unknown” with the same 0.5 ml of solution. And after adding 5ml of Coomassie Blue to each tube (1-5) and to the blank, the result of absorbance was read at 595 nm.

4. What is the most significant finding in the article? Summarize this clearly in several sentences.