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B. what are the symptoms of mulicolanrity and its implication for the properties of the OLS estimations
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- 1. Explain the formation of thioether-linked prenyl anchor proteins and also explain the structure of caveolae.5. Explain why a “sawtooth” pattern is observed in the force-extension curves, versus the singlepeak graph for the RNA molecule in Figure.1. Search for the `P06858` a) Give information about the PTM and Processing of this protein. b) Which position has maximum amount of variations? Give details about the variations. (Eg: What kind of variation? Feature ID? Disease association? etc..)
- After purifying the expressed protien His6-NEP of interest, the different fractions collected will be run on an SDS-Page gels. If our purification was successful a band of correct size should be present in: A) orginal lysate B) lysate flowthrough C) wash flowthrough D) Elute E) orginal lystae and elite fractionsMake a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS analysis of AlaE attached. Describe the purpose of these analyses, how they work, and what your analyses suggest with regards to the topology of AlaE.Discuss the 2 motor proteins associated with the MT in detail, but do not include structure.
- 1. Suppose Biuret test is conducted to a solution of RNA. Will it give a positive result or not? Explain your answer. ____________________________________________________________________ ____________________________________________________________________ 2. Why is Molisch’s test used for the determination of presence of pentose in the hydrolysate? What other test could be used for this? ____________________________________________________________________ ____________________________________________________________________5. The data in the table below were collected during a laboratory session assaying for thekinetics of Acid Phosphatase. Using a simple flow diagram, show how you would design theexperiment and focus on the principles of the assay and the major components for theexperimental design. Concentration (mM) Absorbance1 (410nm) Absorbance2 (410nm)0 0.007 0.0081 0.051 0.0672 0.078 0.0965 0.168 0.1558 0.234 0.25210 0.297 0.31620 0.523 0.514 30 0.759 0.7198. A dipeptide T, obtained from the breakdown of an enzyme found in the human body, is made up of Amino Acids R and S. (a) Classify Amino Acids R and S as an acidic amino acid or a basic amino acid. (b) Name Bond Q. (c) State any ONE type of protein that could be found in human body. (2%) (1%) (1%)
- 1. In the preparation of the standard curve for protein analysis, 50 mnBSA (bovine serum albumin) dissolved in H2O to a final volume of 5mL was used as stock solution. What is the weight of BSA in 0.1 mL ofstock solution? In 0.2 mL? The mentioned aliquots (#9) which were diluted with enough water to a final volume of 1 mL were assayed colorimetrically and yielded the following absorbance readings: mL stock mL H2O Absorbance0.2 0.8 0.1000.4 0.6 0.2000.6 0.4 0.3000.8 0.2 0.4001.0 0.0 0.500 i. Tabulate mg BSA vs. absorbance readingii. Draw the curve where mg BSA is on x-axis and absorbance ony-axis (use graphing paper).iii. Calculate the slope of the curveiv. If a sample solution has an absorbance of 0.332, determinemg protein of the sample.What is the approximate rate of change of A340 measured? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 30 ml of the ADH solution was tested in the same way (i.e. half the amount of protein) ? i.e. ΔA340 / min = __________ What rate of change of A340 would you predict if 60 ml of a 0.5 μM ADH solution was tested in the same way? i.e. ΔA340 / min = __________ As well as writing your answers, explain your reasoning.3 i. Define Biological Value (BV) of Proteinsii. What are the advantages of this protein evaluation method when compared to Nbalance and chemical score?5iii. A bull consumes 7 kg DM of feed containing 60 g N/kg. The bull excretes 120 g N inthe faeces and 50 g N in the urine. The feacal and urinary N had 20 g metabolic feacalnitrogen (MFN) and 6 g endogenous urinary nitrogen (EUN) respectively.Calculate the BV of the protein in the diet.