Make a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS analysis of AlaE attached. Describe the purpose of these analyses, how they work, and what your analyses suggest with regards to the topology of AlaE.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter31: Completing The Protein Life Cycle: Folding, Processing, And Degradation
Section: Chapter Questions
Problem 4P
icon
Related questions
Question

Make a figure that includes the hydropathy profile analysis, the TMHMM analysis and the TOPCONS analysis of AlaE attached.

Describe the purpose of these analyses, how they work, and what your analyses suggest with regards to the topology of AlaE.

Score
3.5
3
2.5
2
1.5
1
0.5
0
-0.5
-1
-1.5
20
ProtScale output for user_sequence
40
Hydropath. / Kyte, & Doolittle
60
80
Position
100
120
140
Transcribed Image Text:Score 3.5 3 2.5 2 1.5 1 0.5 0 -0.5 -1 -1.5 20 ProtScale output for user_sequence 40 Hydropath. / Kyte, & Doolittle 60 80 Position 100 120 140
Predicted topologies and predicted AG values:
TOPCONS
OCTOPUS
Philius
PolyPhobius
SCAMPI
SPOCTOPUS
PDB-homology ***No homologous TM proteins detected***
TOPCONS
- Inside - Outside TM-helix (IN->OUT) TM-helix (OUT->IN) Signal peptide
Consensus prediction (TOPCONS):
Reliability
0.9
20
20
40
40
60
60
80
80
Position
100
100
120
120
140
10
140
8
6
2
0
AG (kcal/mol)
High-resolution image
High-resolution image
Transcribed Image Text:Predicted topologies and predicted AG values: TOPCONS OCTOPUS Philius PolyPhobius SCAMPI SPOCTOPUS PDB-homology ***No homologous TM proteins detected*** TOPCONS - Inside - Outside TM-helix (IN->OUT) TM-helix (OUT->IN) Signal peptide Consensus prediction (TOPCONS): Reliability 0.9 20 20 40 40 60 60 80 80 Position 100 100 120 120 140 10 140 8 6 2 0 AG (kcal/mol) High-resolution image High-resolution image
Expert Solution
steps

Step by step

Solved in 3 steps with 14 images

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning