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- What are the importance of accurate wavelength measurement in purified DNA sample?
- Explain why contaminants must be avoided in DNA samples.
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- What are the importance of accurate wavelength measurement in purified DNA sample?What does the 260 nm light detect when measuring the purity of a DNA sample? Please select the single answer that is most correct.In gel electrophoresis and column chromatography, why do large molecules migrate more easily in one method and with more difficulty in the other? Which method generated the most precise results?
- Why is polyacrylamide gel is used in electrophoresis?In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.
- What are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).Please briefly explain what gel electrophoresis is and how it works to separate a mixed sample of macromolecules like DNA.In which reagent is extracted DNA suspended to put it in solution? A. Sodium citrate saline buffer B. Sodium dodecyl sulfate (SDS) C. Cold absolute alcohol D. HCl E. H2SO4
- Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?When you isolate DNA, what is the purpose of the hot water and the detergent in the activity?Why is it important that the agarose is exposed to ethidium bromide, which is either added to the gel itself or mixed to the staining solution? Explain the principle behind its use.