1. Acid phosphatases are an important of enzymes that can be detected in group human blood serum, Under slightly acidic conditions (pH 5,0), this group of enzymes can hydrolyze biological phosphate esters as follows: R-0-P-03-2 + H 20 → R-OH + HO- P-03-2. Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostrate gland. The enzyme from the prostrate gland is clinically important because an increased activity in the blood is frequently an indication of can- cer of the prostrate gland. Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acid phosphatases from other tissues. How can you use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostrate gland in human blood serum?

Biology: The Dynamic Science (MindTap Course List)
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ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
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Chapter6: Energy, Enzymes, And Biological Reactions
Section: Chapter Questions
Problem 7TYK: In an enzymatic reaction: a. the enzyme leaves the reaction chemically unchanged. b. if the enzyme...
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1. Acid phosphatases are an important
of enzymes that can be detected in
group
human blood serum, Under slightly acidic
conditions (pH 5,0), this group of enzymes
can hydrolyze biological phosphate esters as
follows: R-0-P-03-2 + H 20 → R-OH + HO-
P-03-2. Acid phosphatases are produced
and can be detected in erythrocytes, kidney,
spleen, the liver, and prostrate gland. The
enzyme from the prostrate gland is clinically
important because an increased activity in
the blood is frequently an indication of can-
cer of the prostrate gland. Tartrate ion can
strongly inhibit the phosphatase from the
prostrate gland, but not acid phosphatases
from other tissues. How can you use the
information above to develop a specific
procedure for measuring the activity of the
acid phosphatase of the prostrate gland in
human blood serum?
Transcribed Image Text:1. Acid phosphatases are an important of enzymes that can be detected in group human blood serum, Under slightly acidic conditions (pH 5,0), this group of enzymes can hydrolyze biological phosphate esters as follows: R-0-P-03-2 + H 20 → R-OH + HO- P-03-2. Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostrate gland. The enzyme from the prostrate gland is clinically important because an increased activity in the blood is frequently an indication of can- cer of the prostrate gland. Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acid phosphatases from other tissues. How can you use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostrate gland in human blood serum?
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