1. Benedicts test is used to identify reducingsugars. What is the correct principle?A. Heating of simple carbohydrate is5. A technique in genetic testing in whichDNA fragments are amplified to moreuseful.added with Copper (II) formingcopper (1) oxide which is an orange/brick red complex.A. Restriction endonucleasesB. CloningC. ProbesD. Southern blottingB. Polviodide ions is added tocarbohydrate forming a blackcolored absorption complex, dextrin.C. Carbohydrate is added to H25046. The wrong monosaccharide combinationundergoing dehydration and creatingis.A. Sucrose = Fructose + galactoseB. Lactose = galactese + glucoseC. Maltose = Glucose + glucoseD. All of the above.furfural which is combined withalpha naphtol creating a purplecolored complex.D. None of the above.7. Arrange the correct sequence in southern2. What reagent is added to a sugar-jovertass solution in order to quantifyits reaction seectrophotometrically?A. Benedict's solution.B. Biuret's reagent.C. Bile esculin reagent.D. Rinitrosalicyxlis acid.blotting.I. DNA extracted from white cells and arecleaved.II. bubridization with p-labeled probe.II. Agarose gel electrophoresis.IV. Blotting techniqueV. Expose of x-ray film.3. Determine the ph of a 0.50 M NAH2PO4and 0.10 M N22HPO4 solution. Rka is 7.21.A. I, II, II, V, IVB. I, II, III, IV, V.C. I, IV, II, V, IID. I, II, IV, II, V.А. 5.5В. 6.5С. 4.5D. 7.58. Determine the pH of a 30 g NaH2PO4(120MM) and a 90 g N22HPO4 (142||MM) solution with a gka of 7.21 andtotal volume of 1L.A. 6.40В. 6.61C. 7.40D. 7.614. Determine the pH of a 50 g NaH2PO4(120MM) and a 100 g N22HPO4 (142 MM)solution with a pka of 7.21 and total volumeof 1 L.A. 6.43В. 6.50C. 7.43D. 7.50

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Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter19: Genomes And Proteomes

Section: Chapter Questions

Problem 1TYK

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1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis 2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.
You are setting up your PCR reaction and accidentally add twice as much of the salt buffer as you were supposed to. Select all that apply. 1. How will this impact product formation? 2. In what way(s) will the reaction be altered? (a) ...because primer/template binding will be altered (b) ...because the mechanism of dNTP addition will be altered. (c) You will get more of the desired PCR product... (d) ...because template denaturation will be altered. (e) You will get the same amount of the desired PCR product... (f) You will get less of the desired PCR product...
Please answer these two questions regarding PCR a) First question you must use the image: What is the DNA profile for suspect B for the TH01 locus? b) What determines the target sequence amplified in PCR?
Evaluation of PCR product electrophoresed on 0.8% agarose gel shows non-specific bands. The appropriate modification for the next PCR reaction is to: a) increase the concentration of Taq polymerase b) increase the number of PCR cycles c) decrease the template denaturation temperature d) reduce the concentration of primers
PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genome
An individuals set of unique _______ can be used in DNA profiling. a. DNA sequences b. short tandem repeats c. SNPs d. all of the above
SNP-chips are used ____. a. with short tandem repeat profiling b. to sequence DNA. c. in PCR d. for full genome sequencing e. in DNA profiling
(a) Describe the actions with reason that should be taken under the following situations: (i) An unlabelled collection tube with a requisition for a V Leiden test is received in the laboratory. (ii) After PCR, the amplification control has failed to yield a product. (iii) DNA is stored overnight in a refrigerator set at 8oC but reads 14 oC. (iv) An isolated DNA sample is to be stored for at least 6 months. (v) The expiration date on a reagent has passed. (b) Explain briefly about genetic testing for new-born, including the importance of genetic testing and the disorder that genetic testing can detect.
in Cohen-Boyer's recombinant DNA procedure _______ must be used for both the bacterial DNA and the amphibian DNA _______. a. the same restrictions enzyme, so that the the restriction site are identical in the DNA of each species b. different restriction enzymes, so that the genes outside the restriction site are maintained c. the same restriction enzymes, to ensure that the newly formed DNA can replicate d. different restriction enzymes, to ensure that the newly introducted genes are maintained in the bacterial DNA
RELPs: A. are the same length for mutant and normal beta-globin alleles. b. determine the sequence of bases in a DNA fragment. c. have short, palindromic sequences of DNA in the middle. D. are used as vectors. e. are produced by reaction with restriction enzymes and are detected with PCR and gel electrophoresis.
A linear DNA fragment was produced by digestion with the restriction enzyme, Xba1. This fragment with XbaI(X) sites on both ends was then further digested with HindIII (H) and EcoRI (E). Draw a restriction map of the linear fragment based on the gel electrophoresis results shown below. X H Marker E H/E __2000bp __ __1500b __1300bp __ __ __1000bp __ __700bp __ __500bp __400bp __300bp __ __200bp __ __ __100bp
Sickle cell anaemia is caused by a mutation in the HBB gene. The normal wild-type βA allele contains an MstII restriction site; in the mutated sickle-cell βS allele this restriction site has been lost.PCR amplification of part of the gene encompassing the mutation site from a normal unaffected individual results in a product of 110bp. Digestion of the PCR product with MstII and subsequent gel electrophoresis results in a single band of 55bp. How many bands and of what size would you expect to see in individuals who: i) has sickle-cell anaemia (has two βS alleles) ii) carry the disease (sickle cell trait) (has one βS and one βA allele) a. Individual Number of bands Size of bands (bp) Sickle-cell anaemia(has two βS alleles) 2 55 Sickle cell trait)(has one βS and one βA allele) 1 110 b. Individual Number of bands Size of bands (bp) Sickle-cell anaemia(has two βS alleles) 2 110 Sickle cell trait)(has one βS and one βA allele) 2 55 & 110…

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