Bial's test uses concentrated HCI as a dehydrating acid and orcinol + traces of ferric chloride as condensation reagent. The test reagent dehydrates pentoses to form furfural. Furfural further reacts with orcinol and the iron ion present in the test reagent to produce a O bluish or green product red precipitate yellow solution O light pink or pink solution O Other:
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- Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a blue solution and the recipe for 2X Laemmli buffer is: 0.125 M Tris HCl, pH 6.8, 20% glycerol, 4% SDS and 10% β-mercaptoethanol (βME). What is the purpose of each of these ingredients?Your supervisor asks to make 350 mL of a 0.75% (w/v) starch solution using a 5% (w/v) stock solution. You have calcuated how much stock solution you need. But, how much water (in mL) will you need to add to the stock to achieve the correct final concentration?The above molecule was subjected to acid hydrolysis then was analyzed thru qualitative tests. Indicate whether the given molecule will give a positive (+) or negative (-) result in each given test. Identify whether (+/-) Test for phosphate: Dische Test: Bials Test: Murexide test: Wheeler-Johnson Test: 6. What is the transitory temperature?: Higher or lower?
- You are supplied with the following: / Jy word voorsien van die volgende: NaCl (Mr= 58,443 g /mol) 2.5M Tris-Cl, pH 8 solution /oplossing (1 Litre) EDTA (Mr= 380,2g/mol) 10% sodium dodecyl sulphate solution / natriumdodecyl sulfaat oplossing Proteïnase K solution / oplossing (50 mg dissolved / opgelos in 1 ml ddH2O) You need a digestion buffer consisting of the following: / Jy moet 'n verteringsbuffer op maak wat uit die volgende bestaan: 15m M NaCl 75 mM Tris-Cl,pH 8 16 mM EDTA,pH 8 0.8% sodium sulphate / natrium dodecyl sulfaat 0,75 mg/ml proteïnase K How will you prepare 500 ml of the digestion buffer? Show all your steps and calculations. Remember to explain exactly how you will make it up. / Hoe sal jy 500 ml van die verteringsbuffer voorberei? Wys al jou stappe en berekeninge. Onthou om te verduidelik presies hoe jy dit sal op maak.Determine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml of 0.9% (w/v) saline (NaCl) b) 30 ml of 50% glycerol (v/v) c) Electrophoresis requires TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. TAE is normally made as a 50X concentrated stock. Provide a recipe to make 40 ml of 50X TAE. The recipe for one liter of 50X TAE is as follows: 242g Tris base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTAAn aqueous antifreeze solution is 45.0% ethylene glycol () by mass. The density of the solution is 1.05. Calculate the molality, molarity, and mole fraction of the ethylene glycol
- Complete the table below regarding the different laboratory tests done for Amino Acids and Proteins Tests Procedure (Chemical Reagents Added) Positive Results/ Observations (ex: color of solution, precipitate formation, etc) Positive for (provide details like functional group responsible) Molisch Test Benedict’s Test Barfoed’s Test Moore’s Test Bial’s Test Seliwanoff’s Test Osazone Test Fehling’s Test Tollen’s Test Mucic Acid Test Lugol’s Test/ Iodine Test Hydrolysis w/ Benedict’s Test Hydrolysis w/ Iodine Test Provide the expected results with the samples given below: For negative results just leave it blank, for positive results write “(+) then the expected observation” ex: + blue solution Samples Molisch Test Benedict’s Test Barfoed’s Test Moore’s Test Bial’s Test Seliwanoff’s Test Glucose Galactose Fructose Ribose…. A prescriber ordered 240 mL of ¾ strength Sustacal PO B.I.D. Explain how you would use water as a diluent to mix this solution. Show your work with units and describe how much water and how much Sustacal you would use to create this mixture..Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- Qualitative and Quantitative Tests for Amino Acids and ProteinsComplete the table: Test Reagents Positive Result Positive Indication Ninhydrin test Xanthoproteic acid test Millon's test Hopkins Cole test Pauly's diazo Test Lead sulphide test Histidine test Isatin test Folin’s McCarthy Sullivan Test Nitroprusside TestLipids from an organic sample are extracted separately by column chromatography using the ff eluants: 1st eluant - chloroform: methanol: water (6:3:1) 2nd eluant - methanol: chloroform (9:1) 3rd eluant – petroleum ether: ethyl ether (1:1) Test results for each eluate are tabulated below. Identify the possible lipid present in each eluate. Test Reagents 1. KHSO4 2. HNO3,(NH4)3MoO4 3. CHCl3, acetic anhydride, conc. H2SO4 4. -naphthol, conc. H2SO4 5. Hydroxylamine HCl, FeCl3 6. ninhydrin reagent POSSIBLE LIPIDS: Triacylglycerol, cholesterol, sphingomyelin, lecithin20 mL solution is 2 M NaOH. If 0.5 M HCl is to be added to this solution, calculate the pH of the resulting solution: A. when 10 mL of the 0.5 M HCl is added to the original solution of the base (initial pH). B. When 50 mL H20 is added to the original solution of the base.