1. Calculate the total volume of 25 mM NaCitrate, and 25 mM NaPhosphate youwill need for both day 1 and day 2.Total volume of 25 mM NaCitrate 940μl +945μl = 1885μlTotal volume of 25 mM NaPhosphate 20μl + 15μl = 35 μl2. Below provide the calculations necessary to produce those solutions from thestock reagents provided. Below are the instructions to make 200mL of 25mMNaCitrate with 0.1%BSA. NOTE: you do not need to make 200mL. Your volumefrom problem 1 will be different. Finally, for the Na Phosphate buffer the MW is141.96 g/mol.25mM NaCitrate pH=4.8, containing 0.1% BSA (200ml)MW= 294.10 g/mol1.4705 grams into 190mL water add 0.2 grams BSA. pH to 4.8 using dilute NaOH or HCIDilute to 200mlI●

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1. Calculate the total volume of 25 mM NaCitrate, and 25 mM NaPhosphate you will need for both day 1 and day 2. Total volume of 25 mM NaCitrate 940μl +945μl = 1885μl Total volume of 25 mM NaPhosphate 20μl + 15μl = 35 μl

1. Calculate the total volume of 25 mM NaCitrate, and 25 mM NaPhosphate you will need for both day 1 and day 2. Total volume of 25 mM NaCitrate 940μl +945μl = 1885μl Total volume of 25 mM NaPhosphate 20μl + 15μl = 35 μl

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter33: Automated Methods Of Analysis

Section: Chapter Questions

Problem 33.8QAP

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Question

1. Calculate the total volume of 25 mM NaCitrate, and 25 mM NaPhosphate you
will need for both day 1 and day 2.
Total volume of 25 mM NaCitrate 940μl +945μl = 1885μl
Total volume of 25 mM NaPhosphate 20μl + 15μl = 35 μl
2. Below provide the calculations necessary to produce those solutions from the
stock reagents provided. Below are the instructions to make 200mL of 25mM
NaCitrate with 0.1%BSA. NOTE: you do not need to make 200mL. Your volume
from problem 1 will be different. Finally, for the Na Phosphate buffer the MW is
141.96 g/mol.
25mM NaCitrate pH=4.8, containing 0.1% BSA (200ml)
MW= 294.10 g/mol
1.4705 grams into 190mL water add 0.2 grams BSA
. pH to 4.8 using dilute NaOH or HCI
Dilute to 200ml
I
●

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