1. Differentiate the tubed media. 2. Discuss the different streaking methods. 3. What is the streaking method used for Antimicrobial Susceptibility testing? 4. How will you determine if a culture is pure or mixed?
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1. Differentiate the tubed media.
2. Discuss the different streaking methods.
3. What is the streaking method used for Antimicrobial Susceptibility testing?
4. How will you determine if a culture is pure or mixed?
Step by step
Solved in 4 steps
- 1. a. Disk A b. Disk B c. Disk C d. Disk D 2. a. Strip A with MIC at 0.25 μg/mL b. Strip B with MIC at 0.064 μg/mL c. Strip C with MIC at 0.075 μg/mL d. All of the strips show equal antimicrobial efficacy1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.Hi, I want to ask the question related to antibiotics resistance? The question is the non-existance of solidifying agent may produce a liquid like culture media. Name the method used to determine the Antibiotic Susceptibility test which uses the said media? Can explain answer? Thank you.
- 3. What needs to be considered in choosing chemical antisepsis agents? A. Removes soil, debris, natural skin oil, hand lotion and microorganism from the dermisDecreases the number of microorganism on the skin to an irreducible minimum Keeps the population of microorganisms minimal during the operative procedure by suppression of growth Reduces the hazard of microbial contamination B. Broad-spectrum antimicrobial agent Fast-acting and effective Non-irritating Prolonged action in preventing the growth of microorganism C. Mild and paraben-free Have little or no residual effect on the skinMost rapid and greatest reduction of microorganisms No surfactant or foaming action D. Scrub sinks should be located adjacent to OR for convenience and safety reasonsScrub sinks should be knee or foot-operated to reduce contamination risks Scrub sinks are deep and wide enough to prevent splashbackScrub sinks are designated as single-use for scrubbing and handwashing and not to be used for clearing…Why is Muller Hinton Agar (MHA) ideal for antibiotic susceptibility testingProvide at least two other examples of samples that are best prepared using Smear PreparationTechnique except for blood.
- 1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.Explain the following tests using Pseudomonas aeruginosa. Describe the purpose, reagents used and the results using Pseudomonas aeruginosa.NOTE: Kindly provide references. 1. Differentiate between defined and undefined culture media. 2. Define alpha, beta, and gamma-hemolytic reactions on blood agar media (BAM). Cite two (2) examples of bacteria causing the above hemolytic reactions.