1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep?
1. Explain why the following steps are essential during sub-culturing:
a. Flaming the inoculating instrument prior to and after each inoculation
b. Holding the test tube caps in the hand as illustrated in Figure 1.
c. Cooling the inoculating instrument prior to obtaining the inoculum
d. Flaming the neck of the tubes immediately after uncapping and before recapping
2. Describe the purposes of the sub-culture procedure.
3. Explain why a straight inoculating needle is used to inoculate an agar deep tube.
4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep?
5. Enumerate the Good
6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
