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1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
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- Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.
- 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.In the Kirby-Bauer test, which of the following must be consistent? a. concentration of bacteria placed on the plate b. type of medium in the plate c. concentration of antimicrobial drug in the disk d. all of these1. Use the words presented in below to create a concept map illustrating the relationships among these key terms – refer to “Five I’s” by Cowan and Smith (2018) to be guided. inoculation identification biochemical tests streak plate isolation medium subculturing mixed culture incubation multiplication source of microbes pure culture inspection staining transport medium wire loop 2. What is the importance of employing aseptic techniques? Give an example of a situation in the laboratory that might happen when this method is not practiced.
- 1. Answer the following questions A. What is the antimicrobial mode of action in the antimicrobial assay? B. Based on the results, interpret the efficacy on the test organism?1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.1. List the purpose(s) of the following: • use of Dey-Engley medium in environmental swabs• addition of K2SO3 to the pre-enrichment medium for garlic flakes• addition of Triton X to lactose broth for ground meat
- 1. List the different reagent strip tests with their principle 2. Enumerate 5 clinical significance for each reagent strip tests2. Gamma-hemolysis on a blood agar plate would likely appear: Select one: a. As no change in the medium b. Blue c. Yellow d. Green e. As a clearing.1. Give at least five observable metabolic activities or phenotypes of an organisms that are determined by the MicroSEQ™ ID microbial identification system. Please, do not just copy from somewhere. Answe this comprehensively.