1. How much NaOH(in grams) is reguired to prepare 0.2 M of 250 m! NaOH?(Naoh Ma=40g/mol)2. AWhite blood cell Suspension is dyed by Trypan Blue in 1:1 Ratio(Volume).7 square is counted and the values are;Total Viable Cells: 168Total Nonviable Cells:32a.Percentage of Viable Cells: ?b.Average Number Of cells per square: ?ç.Dilution Factor:?d.Concentration (Vable Cells/ml): ?3. Amniotic fluid(AF) was taken from the motherfor the baby with suspected disease. Cells werecounted in the amniotic fluid, the sample wasplaced on the Thoma slide. There were too manycells. Only 6 randomly counted on the secondsize of square areas; Number of cells in this AFsample found 97, 123, 113, 72,60, 105.(100 µl Sample Dilluted with 200 µl Trypan Blue.)a. Average of cells = ?b. Number of cells in 1 ml=?0,0250,051 mmFigure 1: Area Number 1: x10.000; Area Number 2: x250.000.

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1. How much NaOH(in grams) is required to prepare 0.2 M of 250 m! NaOH?(Naoh Ma= 40g/mo)

Cardiopulmonary Anatomy & Physiology

7th Edition

ISBN:9781337794909

Author:Des Jardins, Terry.

Publisher:Des Jardins, Terry.

Chapter15: Hemodynamic Measurements

Section: Chapter Questions

Problem 1RQ

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Question

1. How much NaOH(in grams) is reguired to prepare 0.2 M of 250 m! NaOH?(Naoh Ma=
40g/mol)
2. AWhite blood cell Suspension is dyed by Trypan Blue in 1:1 Ratio(Volume).
7 square is counted and the values are;
Total Viable Cells: 168
Total Nonviable Cells:32
a.Percentage of Viable Cells: ?
b.Average Number Of cells per square: ?
ç.Dilution Factor:?
d.Concentration (Vable Cells/ml): ?
3. Amniotic fluid(AF) was taken from the mother
for the baby with suspected disease. Cells were
counted in the amniotic fluid, the sample was
placed on the Thoma slide. There were too many
cells. Only 6 randomly counted on the second
size of square areas; Number of cells in this AF
sample found 97, 123, 113, 72,60, 105.
(100 µl Sample Dilluted with 200 µl Trypan Blue.)
a. Average of cells = ?
b. Number of cells in 1 ml=?
0,025
0,05
1 mm
Figure 1: Area Number 1: x10.000; Area Number 2: x250.000.

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